CAZyme Information: MGYG000003504_00436

Basic Information

• Species: CAG-1435 sp003537755
• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; CAG-314; CAG-1435; CAG-1435 sp003537755
• CAZyme ID: MGYG000003504_00436
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
585		66990.2	6.6615

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003504	2246614	MAG	Fiji	Oceania

• Gene Location: Start: 11735; End: 13492 Strand: -

Full Sequence      

MPTTGLKTVWGEQLDDKPLQEYPRPQFVRNSYINLNGWWDYAITKNDTMPAVFDGRILVP
FSPESILSGVGRQLKPRERLFYHTEFTLSRSFVRDVILLNFGAVDCICDVFVNEIKVCHH
EGGYLPFTADITSAAKIDQPNSLVVKVIDYTDTRYYQCGKQSTHRGGMWYTPQSGIWQTV
WLESVPTDYIKSLRITPVYDEGRVIVETDCTFDEPVTFILKEGDEEIAKVDGKGTVSLSF
PDGRFRSWTPETPFLYDLVLISRRDKVTSYFAMRKFGCESVGRHKRLMLNNKPYFHNGLL
DQGYWSDGLYTAPSDEALVADIALAKRLGFNMLRKHIKVEPARWYYHCDRLGMLVWQDMP
SGGTKQNLLVTLALPYSGKRNVKDSRYGLFSRRQKISRDAFEKEYTEMIGTLYNCPCIAM
WVPFNEGWGQFDANRIAALTKQIDGTRTVDHASGWHDQGGGDINSMHVYFKATKLHKDKH
NRVTCLTEFGGYSLPISGHIFNTEREYGYKKFDDVEQYNIAIADLYKHDVIDLVPEGLSA
AVLAQLSDVEDEVNGLVTYDRKVLKVDETLMQTLNGQIRIGKEWI

Enzyme Prediction     

No EC number prediction in MGYG000003504_00436.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	26	466	6.5e-91	0.49601063829787234

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10150	PRK10150	5.30e-25	81	449	68	445	beta-D-glucuronidase; Provisional
COG3250	LacZ	6.91e-25	82	449	67	429	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	8.27e-16	82	449	113	472	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	2.05e-13	123	461	161	491	beta-galactosidase.
pfam02837	Glyco_hydro_2_N	5.43e-09	10	186	8	169	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AEC02401.1	4.54e-209	3	581	13	593
AOH43755.1	2.10e-199	6	579	1	570
SCG46038.1	4.33e-199	6	580	5	583
AYF29402.1	1.51e-197	6	579	3	581
QCI85692.1	3.48e-197	3	578	4	579

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7SF2_A	9.80e-144	6	578	12	582	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_C Chain C, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_D Chain D, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_E Chain E, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_F Chain F, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838]
6ED2_A	2.35e-18	82	426	95	441	ChainA, Glycosyl hydrolase family 2, TIM barrel domain protein [Faecalibacterium duncaniae]
5EUV_A	4.83e-18	35	449	7	389	ChainA, Beta-D-galactosidase [Paracoccus sp. 32d],5EUV_B Chain B, Beta-D-galactosidase [Paracoccus sp. 32d],5LDR_B Chain B, Beta-D-galactosidase [Paracoccus sp. 32d]
5LDR_A	4.83e-18	35	449	8	390	ChainA, Beta-D-galactosidase [Paracoccus sp. 32d]
6D1N_A	2.79e-17	36	360	52	380	Apostructure of Bacteroides uniformis Beta-glucuronidase 1 [Bacteroides uniformis],6D1N_B Apo structure of Bacteroides uniformis Beta-glucuronidase 1 [Bacteroides uniformis],6D41_A Bacteriodes uniformis beta-glucuronidase 1 bound to D-glucaro-1,5-lactone [Bacteroides uniformis],6D41_B Bacteriodes uniformis beta-glucuronidase 1 bound to D-glucaro-1,5-lactone [Bacteroides uniformis],6D6W_A Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D6W_B Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D6W_C Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D6W_D Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D7F_A Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_B Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_C Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_D Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_E Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_F Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q6LL68	4.96e-15	82	463	120	493	Beta-galactosidase OS=Photobacterium profundum (strain SS9) OX=298386 GN=lacZ PE=3 SV=1
Q7MG04	1.14e-14	82	463	121	494	Beta-galactosidase OS=Vibrio vulnificus (strain YJ016) OX=196600 GN=lacZ PE=3 SV=1
Q8D4H3	1.51e-14	82	463	121	495	Beta-galactosidase OS=Vibrio vulnificus (strain CMCP6) OX=216895 GN=lacZ PE=3 SV=2
T2KPJ7	9.34e-14	22	493	43	520	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P23989	3.79e-12	82	451	124	483	Beta-galactosidase OS=Streptococcus thermophilus OX=1308 GN=lacZ PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.998383	0.001623	0.000032	0.000006	0.000002	0.000010

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003504_00436.