CAZyme Information: MGYG000004690_00009

Basic Information

• Species: Eubacterium_R sp014465955
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; Eubacterium_R; Eubacterium_R sp014465955
• CAZyme ID: MGYG000004690_00009
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
528		60453.8	6.2305

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004690	1726698	MAG	Spain	Europe

• Gene Location: Start: 8727; End: 10313 Strand: +

Full Sequence      

MKQVTDLKVWNFRIDSLNFEKQVTLPHTWNVDDNMQVQLYRGKAEYSTEVEIESLKNKTA
VLYFGCAYHTAEVFVNGALAGMHKGSGNTPFEIDITKYAVEGENRITVTVDNYKSKDMLP
YKLNYDWADDGGLTRNVTLTIYDKSDLFDMHVTYSIEKITDMHCGGVLNLDIDCIPQAVG
IEIVDFISNETVISTSAYVDGAVSVPFENLKLWDTQHPNLYVVKVKSENDEISKRIGLRT
IEVNGAKVLLNGKEIYMKGCEWMPGSHPDYGLAEPLSHSIKCLSQLKKAGCVFTRFHWQQ
DTSLFDWCDENGLLVQEEIPYWGQPKKATSAQHEIAKQYADEMVYYHSHHPSIICWGVGN
ELGGREKPTIKYVDEMYKYFKSKDSSRLVNYVSCTMHFKKLFAKDDAALHGDIAMWNEYL
GLWMKCKNIKQTIIESYNRCGNMPSMVTEFGLCEPHFKGGDERRTEILLERMPIYKSLPN
MVGYVWFSLNDYRTHCGEAGKGKMNQRIHGSTDLYGNEKPSYQVFKDC

Enzyme Prediction     

No EC number prediction in MGYG000004690_00009.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	9	506	7e-78	0.5811170212765957

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	6.07e-48	10	451	19	513	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	8.54e-40	2	451	10	510	beta-D-glucuronidase; Provisional
PRK10340	ebgA	1.53e-23	53	391	121	474	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam02837	Glyco_hydro_2_N	5.99e-15	21	139	47	165	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.
pfam02836	Glyco_hydro_2_C	4.49e-14	241	437	1	210	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AIQ45707.1	2.35e-127	23	527	41	557
AIQ51262.1	5.67e-125	23	527	15	531
QHQ59621.1	3.41e-122	7	528	8	552
AIQ57030.1	2.34e-120	12	527	33	560
AIQ39960.1	1.17e-119	23	527	44	560

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7VQM_A	4.83e-45	1	494	59	549	ChainA, GH2 beta-galacturonate AqGalA [Aquimarina sp.],7VQM_B Chain B, GH2 beta-galacturonate AqGalA [Aquimarina sp.],7VQM_C Chain C, GH2 beta-galacturonate AqGalA [Aquimarina sp.],7VQM_D Chain D, GH2 beta-galacturonate AqGalA [Aquimarina sp.]
6D89_A	1.02e-40	5	494	68	552	Bacteroidesuniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis],6D89_B Bacteroides uniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis],6D89_C Bacteroides uniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis],6D89_D Bacteroides uniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis]
6D1N_A	1.11e-40	5	494	76	560	Apostructure of Bacteroides uniformis Beta-glucuronidase 1 [Bacteroides uniformis],6D1N_B Apo structure of Bacteroides uniformis Beta-glucuronidase 1 [Bacteroides uniformis],6D41_A Bacteriodes uniformis beta-glucuronidase 1 bound to D-glucaro-1,5-lactone [Bacteroides uniformis],6D41_B Bacteriodes uniformis beta-glucuronidase 1 bound to D-glucaro-1,5-lactone [Bacteroides uniformis],6D6W_A Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D6W_B Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D6W_C Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D6W_D Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D7F_A Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_B Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_C Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_D Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_E Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_F Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis]
6D8K_A	3.22e-39	22	494	79	556	Bacteroidesmultiple species beta-glucuronidase [Bacteroides ovatus],6D8K_B Bacteroides multiple species beta-glucuronidase [Bacteroides ovatus],6D8K_C Bacteroides multiple species beta-glucuronidase [Bacteroides ovatus],6D8K_D Bacteroides multiple species beta-glucuronidase [Bacteroides ovatus]
6NCY_A	2.01e-35	25	493	89	551	Crystalstructure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans [Fusicatenibacter saccharivorans],6NCY_B Crystal structure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans [Fusicatenibacter saccharivorans],6NCZ_A Crystal structure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans bound to phenyl-thio-beta-D-glucuronide [Fusicatenibacter saccharivorans],6NCZ_B Crystal structure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans bound to phenyl-thio-beta-D-glucuronide [Fusicatenibacter saccharivorans],6NCZ_C Crystal structure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans bound to phenyl-thio-beta-D-glucuronide [Fusicatenibacter saccharivorans],6NCZ_D Crystal structure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans bound to phenyl-thio-beta-D-glucuronide [Fusicatenibacter saccharivorans],6NCZ_E Crystal structure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans bound to phenyl-thio-beta-D-glucuronide [Fusicatenibacter saccharivorans],6NCZ_F Crystal structure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans bound to phenyl-thio-beta-D-glucuronide [Fusicatenibacter saccharivorans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KPJ7	4.32e-36	6	362	66	431	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P77989	9.11e-30	10	358	23	384	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
P26257	1.15e-29	13	526	23	542	Beta-galactosidase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=lacZ PE=1 SV=1
P06760	4.51e-20	9	420	44	501	Beta-glucuronidase OS=Rattus norvegicus OX=10116 GN=Gusb PE=1 SV=1
O18835	3.33e-19	19	451	101	541	Beta-glucuronidase OS=Canis lupus familiaris OX=9615 GN=GUSB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000049	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004690_00009.