You are browsing environment: HUMAN GUT
CAZyme Information: MGYG000000779_00053
You are here: Home > Sequence: MGYG000000779_00053
Basic Information |
Genomic context |
Full Sequence |
Enzyme annotations |
CAZy signature domains |
CDD domains |
CAZyme hits |
PDB hits |
Swiss-Prot hits |
SignalP and Lipop annotations |
TMHMM annotations
Basic Information help
| Species | Prevotella sp000431975 | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Lineage | Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella sp000431975 | |||||||||||
| CAZyme ID | MGYG000000779_00053 | |||||||||||
| CAZy Family | GH20 | |||||||||||
| CAZyme Description | Chitobiase | |||||||||||
| CAZyme Property |
|
|||||||||||
| Genome Property |
|
|||||||||||
| Gene Location | Start: 47306; End: 49798 Strand: - | |||||||||||
Full Sequence Download help
| MKCCVTLSLC LGFSLPMAAQ VAMHYQKMVQ GKDGYQSRFV LKNTSGQPLK NDWTIYFSQL | 60 |
| PQAVQNVGTT AVKIWPVNAN FFAMAPTEAW TDLQPGDSLV FDASFGFKMY KTSQQPEGWF | 120 |
| FVGSDGKPAA VPCTYAPFAD SEYQRGVEYK WQRFSGVTDK ALSPTDVLPA VKQARMLSGT | 180 |
| CMLDKGFRME ADEAVAQEGR LLKEKLEKEA RLSYNDKGVR IVLSVDNQLK GMETAGTITY | 240 |
| NGLPSPADEY YELTVGKKAI TIKGRTPHAV WDGAMTLLAL LRNPVNRASL ACMEIKDQPD | 300 |
| FAFRSMMLDV GRNFTPQKEL KKLVDVLTEY KLDYLHLHLV DDEGWRLEIP GIPELTEVGS | 360 |
| RRGYTTDERD CLYPGYDGNF DPKATTVGNG FISRAEYIEL IKYAQARHVQ IIPEIECPGH | 420 |
| SRAAVKSMEA RYHKYKDTDP QKAMEYLLTD FADSSDYEAG SAQGYRNNVM NIALPSTQHF | 480 |
| LRHVLKEVVA MHREAGVPLP VIHLGGDEVA PGSWKKSPAC HAKMRELGFT TTEELSSWFY | 540 |
| SNLIKMMDDS LGVKFAGWQE MFSNHPHLNT AYNRSRAFGV NCWNAVAEWN AADLLFNLAN | 600 |
| QGVPMIVSNV SNTYCDLAYS ANPDEPGLIW GGYVDEATPF NLLPYDPYRS QHDNVAGTAA | 660 |
| YSWKTTPEGA VSLTAEGRRN IVGVQGQLWA ETIRGPQWME YQLFPKALGV LERGWNAHPS | 720 |
| WEAVQGEEAG KAYADALNLW YAKIDKMELP ALASRVNIHV TAPGLRYAGG AVEAAMPMTQ | 780 |
| GKIHYTVDGS EPTAASPVYN GPVKMSEGTF KARFITNGGQ QSAVSAWVVK | 830 |
CAZyme Signature Domains help
| Family | Start | End | Evalue | family coverage |
|---|---|---|---|---|
| GH20 | 297 | 717 | 1.2e-87 | 0.9703264094955489 |
CDD Domains download full data without filtering help
| Cdd ID | Domain | E-Value | qStart | qEnd | sStart | sEnd | Domain Description |
|---|---|---|---|---|---|---|---|
| cd06569 | GH20_Sm-chitobiase-like | 5.93e-180 | 297 | 736 | 1 | 436 | The chitobiase of Serratia marcescens is a beta-N-1,4-acetylhexosaminidase with a glycosyl hydrolase family 20 (GH20) domain that hydrolyzes the beta-1,4-glycosidic linkages in oligomers derived from chitin. Chitin is degraded by a two step process: i) a chitinase hydrolyzes the chitin to oligosaccharides and disaccharides such as di-N-acetyl-D-glucosamine and chitobiose, ii) chitobiase then further degrades these oligomers into monomers. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself. |
| cd06563 | GH20_chitobiase-like | 1.26e-99 | 301 | 722 | 1 | 351 | The chitobiase of Serratia marcescens is a beta-N-1,4-acetylhexosaminidase with a glycosyl hydrolase family 20 (GH20) domain that hydrolyzes the beta-1,4-glycosidic linkages in oligomers derived from chitin. Chitin is degraded by a two step process: i) a chitinase hydrolyzes the chitin to oligosaccharides and disaccharides such as di-N-acetyl-D-glucosamine and chitobiose, ii) chitobiase then further degrades these oligomers into monomers. This GH20 domain family includes an N-acetylglucosamidase (GlcNAcase A) from Pseudoalteromonas piscicida and an N-acetylhexosaminidase (SpHex) from Streptomyces plicatus. SpHex lacks the C-terminal PKD (polycystic kidney disease I)-like domain found in the chitobiases. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself. |
| pfam00728 | Glyco_hydro_20 | 3.75e-94 | 301 | 717 | 1 | 345 | Glycosyl hydrolase family 20, catalytic domain. This domain has a TIM barrel fold. |
| COG3525 | Chb | 1.38e-89 | 26 | 829 | 8 | 716 | N-acetyl-beta-hexosaminidase [Carbohydrate transport and metabolism]. |
| cd06568 | GH20_SpHex_like | 1.01e-57 | 301 | 723 | 1 | 324 | A subgroup of the Glycosyl hydrolase family 20 (GH20) catalytic domain found in proteins similar to the N-acetylhexosaminidase from Streptomyces plicatus (SpHex). SpHex catalyzes the hydrolysis of N-acetyl-beta-hexosaminides. An Asp residue within the active site plays a critical role in substrate-assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself. Proteins belonging to this subgroup lack the C-terminal PKD (polycystic kidney disease I)-like domain found in the chitobiases. |
CAZyme Hits help
| Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End |
|---|---|---|---|---|---|
| QNL40218.1 | 7.17e-226 | 35 | 828 | 49 | 834 |
| QDH53746.1 | 6.49e-224 | 35 | 827 | 49 | 834 |
| SCV07433.1 | 5.19e-223 | 35 | 827 | 49 | 834 |
| ALJ48025.1 | 5.19e-223 | 35 | 827 | 49 | 834 |
| QRQ54899.1 | 5.19e-223 | 35 | 827 | 49 | 834 |
PDB Hits download full data without filtering help
| Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
|---|---|---|---|---|---|---|
| 1QBA_A | 9.63e-92 | 39 | 805 | 44 | 842 | BACTERIALCHITOBIASE, GLYCOSYL HYDROLASE FAMILY 20 [Serratia marcescens],1QBB_A BACTERIAL CHITOBIASE COMPLEXED WITH CHITOBIOSE (DINAG) [Serratia marcescens] |
| 1C7T_A | 1.85e-91 | 39 | 805 | 44 | 842 | ChainA, BETA-N-ACETYLHEXOSAMINIDASE [Serratia marcescens] |
| 1C7S_A | 9.52e-91 | 39 | 805 | 44 | 842 | ChainA, BETA-N-ACETYLHEXOSAMINIDASE [Serratia marcescens] |
| 6EZR_A | 8.43e-69 | 235 | 721 | 195 | 616 | Crystalstructure of GH20 Exo beta-N-Acetylglucosaminidase from Vibrio harveyi [Vibrio harveyi],6EZR_B Crystal structure of GH20 Exo beta-N-Acetylglucosaminidase from Vibrio harveyi [Vibrio harveyi],6EZS_A Crystal structure of GH20 Exo beta-N-Acetylglucosaminidase from Vibrio harveyi in complex with N-acetylglucosamine [Vibrio harveyi],6EZS_B Crystal structure of GH20 Exo beta-N-Acetylglucosaminidase from Vibrio harveyi in complex with N-acetylglucosamine [Vibrio harveyi],6K35_A Crystal structure of GH20 exo beta-N-acetylglucosaminidase from Vibrio harveyi in complex with NAG-thiazoline [Vibrio harveyi],6K35_B Crystal structure of GH20 exo beta-N-acetylglucosaminidase from Vibrio harveyi in complex with NAG-thiazoline [Vibrio harveyi] |
| 6EZT_A | 1.04e-67 | 235 | 721 | 192 | 613 | Crystalstructure of GH20 Exo beta-N-Acetylglucosaminidase D437A inactive mutant from Vibrio harveyi [Vibrio harveyi],6EZT_B Crystal structure of GH20 Exo beta-N-Acetylglucosaminidase D437A inactive mutant from Vibrio harveyi [Vibrio harveyi] |
Swiss-Prot Hits download full data without filtering help
| Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
|---|---|---|---|---|---|---|
| Q54468 | 6.21e-90 | 39 | 805 | 71 | 869 | Chitobiase OS=Serratia marcescens OX=615 GN=chb PE=1 SV=1 |
| P49007 | 3.00e-79 | 223 | 744 | 254 | 752 | Beta-hexosaminidase B OS=Pseudoalteromonas piscicida OX=43662 GN=nag096 PE=3 SV=1 |
| Q04786 | 3.81e-77 | 35 | 722 | 41 | 723 | Beta-hexosaminidase OS=Vibrio vulnificus OX=672 GN=hex PE=3 SV=1 |
| P13670 | 7.40e-74 | 52 | 789 | 82 | 846 | N,N'-diacetylchitobiase OS=Vibrio harveyi OX=669 GN=chb PE=1 SV=1 |
| P96155 | 1.67e-60 | 244 | 714 | 203 | 604 | Beta-hexosaminidase OS=Vibrio furnissii OX=29494 GN=exoI PE=1 SV=1 |
SignalP and Lipop Annotations help
This protein is predicted as SP
| Other | SP_Sec_SPI | LIPO_Sec_SPII | TAT_Tat_SPI | TATLIP_Sec_SPII | PILIN_Sec_SPIII |
|---|---|---|---|---|---|
| 0.000281 | 0.999114 | 0.000160 | 0.000160 | 0.000147 | 0.000137 |