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CAZyme Information: MGYG000002529_00390

You are here: Home > Sequence: MGYG000002529_00390

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species Aeromonas veronii
Lineage Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Aeromonadaceae; Aeromonas; Aeromonas veronii
CAZyme ID MGYG000002529_00390
CAZy Family GH20
CAZyme Description N,N'-diacetylchitobiase
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
887 MGYG000002529_2|CGC1 98288.13 5.8898
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000002529 4703176 Isolate China Asia
Gene Location Start: 49658;  End: 52321  Strand: +

Full Sequence      Download help

MNLKHSLLAI  AMSTVFVSQV  QAADAAKQAV  VDSLANNLVV  KYEVVTNDGA  GAGLDCQALG60
SEWASCGVAK  LHLTNTGADV  TSKDWSIYVS  SIRRIQRVDN  DQFTITHLTG  DLYRLTPTEK120
FQGFAKDATV  EVPLVVEYWV  LFESDIMPNW  YVASEGAEPK  VLASMSNIND  ASTYEKPMPA180
DGWKRTKDDK  NILMNSETRF  AANQTSTLLP  AGKIDNQILP  TPMKQVVKAG  PQVDFSTIKL240
NALDLPSDRA  EALKAQLTKL  GVTLSDTGYP  VTIKLGSKLK  QAEGYDMTIG  QKGTVIQGRD300
IDGAFWGAQS  LISLLGVDDK  LVSPMTVEDA  PRFEYRGMQT  DVARHFRSLD  TMKKLVDQMS360
AMKLNVLHLG  LTNDEGWRLE  IPGLPELTQV  GSQRCHDESE  TKCLMPQLGS  GPTSDNQGSG420
FYSKSDYVDL  VRYAKARGVT  VIPEINMPAH  ARAAVISMEA  RYKRLMAEGK  EAEANQFRLT480
DPADTSNVTS  VQFYDKMSFI  NPCQPGAATF  VAKVMDEVAQ  MHQAAGQPLT  AWHYGGDEAK540
NIMQGGGYQD  PAVTKKEELV  AWKGNVDSSK  QDKPFGKSPM  CQKMIDEGKI  KEVGELPVYF600
AKEVGQMVKD  RGFSTLQAWE  DGLKYASGAK  DFATDNTRVN  FWETLYWGGF  NEAMKWAHKG660
YQVVLSNPDY  LYFDFPNEVH  PAERGYYWAT  RFNDTRKVFA  FAPENLPQNA  ETSVDRDGNA720
FVAKGDQDPV  KFKGISGQQW  SETVRTDAQY  EYMVYPRIFS  VAERAWHKGG  FELDYVKNRE780
FSGETKFVNK  ATLNKEWNQF  ANVLGQRVLP  KLDQAGVEYR  LSVPGAKVVN  GVLEANVDLP840
GLPIQYSLDG  KTWTAYDAAA  KPSVNGKVYL  RTTSFDGKRT  SRVTELN887

Enzyme Prediction      help

EC 3.2.1.52

CAZyme Signature Domains help

Created with Snap4488133177221266310354399443487532576620665709753798842326768GH20
Family Start End Evalue family coverage
GH20 326 768 3.6e-98 0.9792284866468842

CDD Domains      download full data without filtering help

Created with Snap4488133177221266310354399443487532576620665709753798842329804GH20_Sm-chitobiase-like57884Chb333768Glyco_hydro_20333768GH20_chitobiase-like38196CHB_HEX
Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
cd06569 GH20_Sm-chitobiase-like 0.0 329 804 1 445
The chitobiase of Serratia marcescens is a beta-N-1,4-acetylhexosaminidase with a glycosyl hydrolase family 20 (GH20) domain that hydrolyzes the beta-1,4-glycosidic linkages in oligomers derived from chitin. Chitin is degraded by a two step process: i) a chitinase hydrolyzes the chitin to oligosaccharides and disaccharides such as di-N-acetyl-D-glucosamine and chitobiose, ii) chitobiase then further degrades these oligomers into monomers. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.
COG3525 Chb 2.97e-144 57 884 8 732
N-acetyl-beta-hexosaminidase [Carbohydrate transport and metabolism].
pfam00728 Glyco_hydro_20 4.47e-133 333 768 1 345
Glycosyl hydrolase family 20, catalytic domain. This domain has a TIM barrel fold.
cd06563 GH20_chitobiase-like 1.18e-98 333 768 1 344
The chitobiase of Serratia marcescens is a beta-N-1,4-acetylhexosaminidase with a glycosyl hydrolase family 20 (GH20) domain that hydrolyzes the beta-1,4-glycosidic linkages in oligomers derived from chitin. Chitin is degraded by a two step process: i) a chitinase hydrolyzes the chitin to oligosaccharides and disaccharides such as di-N-acetyl-D-glucosamine and chitobiose, ii) chitobiase then further degrades these oligomers into monomers. This GH20 domain family includes an N-acetylglucosamidase (GlcNAcase A) from Pseudoalteromonas piscicida and an N-acetylhexosaminidase (SpHex) from Streptomyces plicatus. SpHex lacks the C-terminal PKD (polycystic kidney disease I)-like domain found in the chitobiases. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.
pfam03173 CHB_HEX 1.68e-78 38 196 1 152
Putative carbohydrate binding domain. This domain represents the N terminal domain in chitobiases and beta-hexosaminidases EC:3.2.1.52. It is composed of a beta sandwich structure that is similar in structure to the cellulose binding domain of cellulase from Cellulomonas fimi. This suggests that this may be a carbohydrate binding domain.

CAZyme Hits      help

Created with Snap44881331772212663103543994434875325766206657097537988421887QHC07616.1|GH201887QMS77897.1|GH201887QSR48271.1|GH201887QHB82021.1|GH201887ATY81822.1|GH20
Hit ID E-Value Query Start Query End Hit Start Hit End
QHC07616.1 0.0 1 887 1 887
QMS77897.1 0.0 1 887 1 887
QSR48271.1 0.0 1 887 1 887
QHB82021.1 0.0 1 887 1 887
ATY81822.1 0.0 1 887 1 887

PDB Hits      download full data without filtering help

Created with Snap4488133177221266310354399443487532576620665709753798842288861C7T_A288861QBA_A288861C7S_A2858206EZR_A2858206EZT_A
Hit ID E-Value Query Start Query End Hit Start Hit End Description
1C7T_A 0.0 28 886 2 858
ChainA, BETA-N-ACETYLHEXOSAMINIDASE [Serratia marcescens]
1QBA_A 0.0 28 886 2 858
BACTERIALCHITOBIASE, GLYCOSYL HYDROLASE FAMILY 20 [Serratia marcescens],1QBB_A BACTERIAL CHITOBIASE COMPLEXED WITH CHITOBIOSE (DINAG) [Serratia marcescens]
1C7S_A 2.16e-319 28 886 2 858
ChainA, BETA-N-ACETYLHEXOSAMINIDASE [Serratia marcescens]
6EZR_A 4.08e-48 285 820 211 638
Crystalstructure of GH20 Exo beta-N-Acetylglucosaminidase from Vibrio harveyi [Vibrio harveyi],6EZR_B Crystal structure of GH20 Exo beta-N-Acetylglucosaminidase from Vibrio harveyi [Vibrio harveyi],6EZS_A Crystal structure of GH20 Exo beta-N-Acetylglucosaminidase from Vibrio harveyi in complex with N-acetylglucosamine [Vibrio harveyi],6EZS_B Crystal structure of GH20 Exo beta-N-Acetylglucosaminidase from Vibrio harveyi in complex with N-acetylglucosamine [Vibrio harveyi],6K35_A Crystal structure of GH20 exo beta-N-acetylglucosaminidase from Vibrio harveyi in complex with NAG-thiazoline [Vibrio harveyi],6K35_B Crystal structure of GH20 exo beta-N-acetylglucosaminidase from Vibrio harveyi in complex with NAG-thiazoline [Vibrio harveyi]
6EZT_A 4.32e-47 285 820 208 635
Crystalstructure of GH20 Exo beta-N-Acetylglucosaminidase D437A inactive mutant from Vibrio harveyi [Vibrio harveyi],6EZT_B Crystal structure of GH20 Exo beta-N-Acetylglucosaminidase D437A inactive mutant from Vibrio harveyi [Vibrio harveyi]

Swiss-Prot Hits      download full data without filtering help

Created with Snap44881331772212663103543994434875325766206657097537988423886sp|P13670|CHB_VIBHA26886sp|Q54468|CHB_SERMA31886sp|Q04786|HEX_VIBVL28805sp|P49007|HEXB_PSEO7100872sp|P86956|HEX_PINMA
Hit ID E-Value Query Start Query End Hit Start Hit End Description
P13670 0.0 3 886 2 882
N,N'-diacetylchitobiase OS=Vibrio harveyi OX=669 GN=chb PE=1 SV=1
Q54468 0.0 26 886 27 885
Chitobiase OS=Serratia marcescens OX=615 GN=chb PE=1 SV=1
Q04786 4.09e-204 31 886 10 844
Beta-hexosaminidase OS=Vibrio vulnificus OX=672 GN=hex PE=3 SV=1
P49007 4.27e-134 28 805 22 768
Beta-hexosaminidase B OS=Pseudoalteromonas piscicida OX=43662 GN=nag096 PE=3 SV=1
P86956 1.55e-46 100 872 104 887
Putative beta-hexosaminidase OS=Pinctada maxima OX=104660 PE=1 SV=1

SignalP and Lipop Annotations help

This protein is predicted as SP

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
0.000218 0.999151 0.000156 0.000163 0.000150 0.000137

TMHMM  Annotations      help

There is no transmembrane helices in MGYG000002529_00390.