CAZyme Information: MGYG000004693_00050

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; UMGS1375
• CAZyme ID: MGYG000004693_00050
• CAZy Family: GH3
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1432	MGYG000004693_1|CGC1	156608.54	4.3434

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004693	1958211	MAG	Sweden	Europe

• Gene Location: Start: 49291; End: 53589 Strand: +

Full Sequence      

MKMRFRKIMAAILSTAMVAAAIPVSGILHVQASSPKEHIDLSRQVAGEGMVLMENDGALP
LAQGEKVAMFGRAMIDYVRGGGGSGATNVDYTHNILQGMQIKAEEGKISLYQPLVDFYTE
QVSANGITNDANITVTNDMLDAAAENADTAIVTIGRYSSEGSDRTASKGDYYLSDAETEL
LTRVAAKFKKTVVVLNVGAVLDTEWIKNIPGIDSVLMAWQAGMEGGLATADVLVGDVNPS
GKFVDTFAKSYTDYPSSDTFYESSAYVNYEEDIFVGYRYFETFDPEYTKVNYDFGYGLSY
TTFKTDNVNVKVDGENIVTTATVTNTGKVAGKQVVQVYFSAPQGKLGKPGKELAAFAKTK
LLQPGESQELTMTYAISDMSSYDDTGKVQKSAYVMEAGDYHIYVGNSIKDATEQGVRYTY
KVEDTEVTEQLSQQCAPVQLEKRLLADKTYEDLRTSSEETNSHYTITSEGTTKVEAENFL
NSSGNLNVETFYDENMEMKRCLAYLNEAGRYMEYELTAEEAGKYEVIMCMANGYAEIQNC
FTVTVNGVQQPDITFNAQQTGDGNGASEWYNFEECEPFYINLDKGVNLVRFTANRNNPNY
DYMLLRRVGDQDEIYTKVIKAEGENKIEAEKYDAAGSTGNNAVRTEDFTKGDYTGTCLAY
MNYVGNYVSYYLNVEEAGEYNVILNVANGRAAFDFDPGMEVDGTKFPATINAKQTGDGSG
KSEWYNFENLEPVTVTLPKGHSILTLRASEKDKYPNIDYILVEKKQTATQSLKSAAAKKA
RTAEDTPASEPADSGKIMLVDVYNNSELMDDFLAQLSDDQLCNLLGGQSNTGVANTGGMG
NLMEYGIPNAMTADGPQGIRIGTTCTAWPISTLLASTWDVDLVEQVGKAAAIEAHNNGID
IWLAPGMNIHRDPLCGRNFEYYSEDPLITGKMAAAITKGCQSEGVSITLKHFTTNNKETN
RNSSDSRVSERALREIYLKGFEIAVKEAQPWSIMTSYNFLNGIETSENKELLTNITRGEW
GYEGIFMTDWGNNSNHAREVLAGNDVKMPSGSPATLKSALDKGILKRSDLEACAERLMNL
IMKVNIFKDKILDSPTVAIGDDTCFKAAENIIWSQTARGENTSDEDGGKDLGYCDEGAWT
QYQISVAKSGTYSLSARSASNAGGGAFDVLVDGAKIASFKAVNTGGWQKWTTLEAQEIRL
EAGIHTLRIEFTESGSNLNWLHFTRQITQQDLDLQSLQRVASVAEELLTDHAEKYTKDSA
QALQAAIAHAREIEAKENPADKELKQAYEDLVDAIVGLKMRGKKSALEAMIAKADEILAS
DNRYVTATIEGLTEELQQAKAVNEQEDALQNEINEAVRSLTIKVAEARLLGDVDGDGQIT
TKDSVSLLRAAAEIEGLSGQDAASADVNGDGMVDTTDAVVILRYTSEQISGF

Enzyme Prediction     

EC	3.2.1.21	3.2.1.58	3.2.1.37	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	845	1049	5.4e-68	0.9675925925925926
CBM6	1106	1225	1e-27	0.8768115942028986

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG1472	BglX	9.22e-52	846	1090	59	321	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
pfam01915	Glyco_hydro_3_C	2.82e-48	50	277	1	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.
PRK15098	PRK15098	3.70e-44	38	405	383	751	beta-glucosidase BglX.
pfam00933	Glyco_hydro_3	1.90e-35	846	1080	65	316	Glycosyl hydrolase family 3 N terminal domain.
cd04080	CBM6_cellulase-like	4.93e-33	1120	1224	39	144	Carbohydrate Binding Module 6 (CBM6); appended to glycoside hydrolase (GH) domains, including GH5 (cellulase). This family includes carbohydrate binding module 6 (CBM6) domains that are appended to several glycoside hydrolase (GH) domains, including GH5 (cellulase) and GH16, as well as to coagulation factor 5/8 carbohydrate-binding domains. CBM6s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. The CBM6s are appended to GHs that display a diversity of substrate specificities. For some members of this family information is available about the specific substrates of the appended GH domains. It includes the CBM domains of various enzymes involved in cell wall degradation including, an extracellular beta-1,3-glucanase from Lysobacter enzymogenes encoded by the gluC gene (its catalytic domain belongs to the GH16 family), the tandem CBM domains of Pseudomonas sp. PE2 beta-1,3(4)-glucanase A (its catalytic domain also belongs to GH16), and a family 6 CBM from Cellvibrio mixtus Endoglucanase 5A (CmCBM6) which binds to the beta1,4-beta1,3-mixed linked glucans lichenan, and barley beta-glucan, cello-oligosaccharides, insoluble forms of cellulose, the beta1,3-glucan laminarin, and xylooligosaccharides, and the CBM6 of Fibrobacter succinogenes S85 XynD xylanase, appended to a GH10 domain, and Cellvibrio japonicas Cel5G appended to a GH5 (cellulase) domain. GH5 (cellulase) family includes enzymes with several known activities such as endoglucanase, beta-mannanase, and xylanase, which are involved in the degradation of cellulose and xylans. GH16 family includes enzymes with lichenase, xyloglucan endotransglycosylase (XET), and beta-agarase activities. CBM6 is an unusual CBM as it represents a chimera of two distinct binding sites with different modes of binding: binding site I within the loop regions and binding site II on the concave face of the beta-sandwich fold. For CmCBM6 it has been shown that these two binding sites have different ligand specificities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QBE95841.1	4.40e-205	36	1085	48	807
AOZ95010.1	5.43e-205	21	1085	12	803
QIB55852.1	4.63e-204	36	1085	26	785
QMW76281.1	4.63e-204	36	1085	26	785
QAA32414.1	6.98e-199	36	1085	26	807

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5WUG_A	1.79e-124	40	1223	38	954	Expression,characterization and crystal structure of a novel beta-glucosidase from Paenibacillus barengoltzii [Paenibacillus barengoltzii],5WVP_A Expression, characterization and crystal structure of a novel beta-glucosidase from Paenibacillus barengoltzii [Paenibacillus barengoltzii]
2X42_A	2.84e-70	36	415	322	709	Structureof beta-glucosidase 3B from Thermotoga neapolitana in complex with alpha-D-glucose [Thermotoga neapolitana DSM 4359]
2X40_A	2.84e-70	36	415	322	709	Structureof beta-glucosidase 3B from Thermotoga neapolitana in complex with glycerol [Thermotoga neapolitana DSM 4359],2X41_A Structure of beta-glucosidase 3B from Thermotoga neapolitana in complex with glucose [Thermotoga neapolitana DSM 4359]
7MS2_A	1.88e-52	844	1081	34	286	ChainA, Thermostable beta-glucosidase B [Acetivibrio thermocellus AD2],7MS2_B Chain B, Thermostable beta-glucosidase B [Acetivibrio thermocellus AD2]
5WAB_A	2.39e-52	846	1083	36	287	CrystalStructure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_B Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_C Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_D Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P16084	7.65e-179	31	1071	21	811	Beta-glucosidase A OS=Butyrivibrio fibrisolvens OX=831 GN=bglA PE=3 SV=1
P15885	1.86e-106	37	1225	8	904	Beta-glucosidase OS=Ruminococcus albus OX=1264 PE=3 SV=1
Q5BFG8	1.13e-55	844	1097	40	297	Beta-glucosidase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=bglB PE=1 SV=1
E7CY69	4.40e-52	846	1083	36	287	Exo-alpha-(1->6)-L-arabinopyranosidase OS=Bifidobacterium longum OX=216816 GN=apy PE=1 SV=1
P14002	1.03e-51	844	1081	34	286	Thermostable beta-glucosidase B OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=bglB PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000615	0.998407	0.000237	0.000323	0.000218	0.000172

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004693_00050.