CAZyme Information: MGYG000000798_00008

Basic Information

• Species: Collinsella sp900542945
• Lineage: Bacteria; Actinobacteriota; Coriobacteriia; Coriobacteriales; Coriobacteriaceae; Collinsella; Collinsella sp900542945
• CAZyme ID: MGYG000000798_00008
• CAZy Family: GH31
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2137	MGYG000000798_1|CGC1	229661.04	4.4126

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000798	2225766	MAG	China	Asia

• Gene Location: Start: 5591; End: 12004 Strand: +

Full Sequence      

MRRNSIFTKRWVKGSAVLVATAATLVFANPQQAIATPLKGVDSATVSQSASNVVDIDFAD
GIKGRITFLEDGIFRYNVDPKGEFSEYATPRSKSHTAKIQAQPDTSDTYSKPSATVADKG
DTIEITGGKATVILDKATGKMSIKSGDRVVVSESASLDLDKKGTVQTLAKEKSENFFGGG
TQNGRFLHTNQTIAISNTNNWTDGGVASPNPFYWTSDGYGVLRNTFAEGSYDFGSTDSST
VTTLHSENEFDAYYFVATNEGASNVATEMLRDYYKVTGNPVLLPEYGFYLGHLNAYNRDG
WSTTSGQKKWETKGSKSSSEKGDVKYESGMSTGYKLDGTLAAETLNGEGPTVDTENMKAT
DFDRQFSARQVIDDYEDNDMPLGWFLPNDGYGAGYGQNGYYKTGGVNSDGTSSADRLNAV
RANVDNLKKFTEYANSKGVSTGLWTQSNLEPDSNSETPWHLLRDFDAEVKTGGISTLKTD
VAWVGSGYSFGLNGIKTAYDTVTTGANKRPNIITLDGWAGTQRFGGIWTGDQYGGNWEYI
RFHIPTYIGQSLSGNPNIGSDMDGIFGGDPIISARDYQWKTFTPSMLDMDGWGTYRKSPM
THGDPYTGISRFYLKLKAQLMPYIYTSAASAANIDTGNGDAGLPMIRAMLLSDNSEYAAS
TSTQYQYMFGENFLVAPVYQDTQADENGNDIRNGIYLPNYGTDENPTIWIDYFSGKQYRG
GQVLNNYEAPLWKLPLFAKANAIVPMYAENNNPEAVSDTNTKGTDRSQRIVEFFATEGDG
TFTQYEDDGSSIENNTTEDDSYGTIDNISYGEHVSTVYRSKAAGGTATFTAEASQGGYNG
YDSNRTTTFVANVSAKPMSVVAKNGGSALNVVEVDSQETFDTATPEAGQAVYFYSETPNL
NHYGSDVDGTEAERGESFNNTKITTNPKVYVKFAKTDVAAAAQTLELGGFVNADATLTAD
RLNENLSAPTNLAAPEDVTTPTSIKLTWGKVDGATSYDLKVDGTVFAVGDAAEFTHTDLA
YNSKHTYQIRSRNAEGYSAWSDVLEANSALDPWRNVPDAEEITWEGSLYGSHKAELAFDH
EFQSGDGGFHSGGNDLGKALTVDYGRAYKLDKLEYYPRDDAGNGTVTKMRVETSLDGVHW
TSQEVDWARSADCKTVAFDGTVAARYVRMTPLASVGNFFSASEIAIYKTDGTDGFAVGSN
LNKATISDGDYSNMKNYLGLENREPDTPTFGSQIRDHFADLNDNGVYDVYDYSFTMAGLD
GGTKQKGKVAGSLAVIPSKTEVEAGDEITVDVYAADAKNVNALGALVNFKSDQFEFVSES
IAQDGSTAGMENLSREKVQFADRTQTINLAFANRGDGKLYNGTGAVASFKLKAKTAGKVE
LPSTSWLIGPACDALEFASDGTVTIPDVPQPTVAEYGQDAFNITMTNDELTTDDGTNVEK
LIQQKSYNALFDNNEGDNGFEFLWNWSGNWDSEGKLPSYVKLPTTMTFAFKTPSKLDSVE
VVNRNGGNGTVQKIKAVVTFEDGSTQEFAGGEYDSFRARYAFGLSAENKGKKATKVEVTP
LEASGDQMLTLREVNFNYTQGVEAIEGVELGKNQTEFFEGDVTLVDAKATPESAGYPYVT
VESSDPSVVSVSAVQAGDSVSYYLRANKAGKAKITVASVLDPSKTASYEVEVKAGVDTSA
LVAALSKAAGYSESVYTKDSYAALTTAVEAAQKLLDGGQGSYSKKDVADATVKIEKAIDG
LKMRPVDEKILINTPENRKNVKVARFSSEADYEGSNAVNALDGDERTLWHSDWAHGTGMP
QYLSVDLGRDYDLTDVTFLPRQDGGTNGDIFEAEILVSDTADGYEMGTAASMGTFAFDND
GRVLTDRGDWKQMSFGAARGRYVTVKVIHAGGGDVDAYCSMAELKFYGTAVPDPVAKDDL
ATAIEKVDAEVAAGDLKAGDYTEASWTALENALASARAILSDENATQDEVDHALRALESA
RGALEKTPVAPVENPTKKQLKALVKQAKETDTRGKTAESVKVLTDAITYAEDVLGDENAS
DTQIQAAYGQLKLAIESLKDADSGNQGGSGNQGSGNGSNGGNQAGKPAGDKAQGSKKNGS
AIPNTGDQTAATVATVGGLGAIIAAIGAFFHRRSKAK

Enzyme Prediction     

No EC number prediction in MGYG000000798_00008.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH31	491	744	1.7e-48	0.5386416861826698

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06596	GH31_CPE1046	7.90e-147	276	715	1	334	Clostridium CPE1046-like. CPE1046 is an uncharacterized Clostridium perfringens protein with a glycosyl hydrolase family 31 (GH31) domain. The domain architecture of CPE1046 and its orthologs includes a C-terminal fibronectin type 3 (FN3) domain and a coagulation factor 5/8 type C domain in addition to the GH31 domain. Enzymes of the GH31 family possess a wide range of different hydrolytic activities including alpha-glucosidase (glucoamylase and sucrase-isomaltase), alpha-xylosidase, 6-alpha-glucosyltransferase, 3-alpha-isomaltosyltransferase and alpha-1,4-glucan lyase. All GH31 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein.
COG1501	YicI	1.64e-55	73	792	62	710	Alpha-glucosidase, glycosyl hydrolase family GH31 [Carbohydrate transport and metabolism].
pfam01055	Glyco_hydro_31	5.94e-46	428	744	159	442	Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases.
cd08759	Type_III_cohesin_like	1.64e-37	1271	1438	1	167	Cohesin domain, interaction partner of dockerin. Bacterial cohesin domains bind to a complementary protein domain named dockerin, and this interaction is required for the formation of the cellulosome, a cellulose-degrading complex. Two specific calcium-dependent interactions between cohesin and dockerin appear to be essential for cellulosome assembly, type I and type II. This subfamily represents type III cohesins and closely related domains.
cd06589	GH31	1.01e-35	363	614	21	265	glycosyl hydrolase family 31 (GH31). GH31 enzymes occur in prokaryotes, eukaryotes, and archaea with a wide range of hydrolytic activities, including alpha-glucosidase (glucoamylase and sucrase-isomaltase), alpha-xylosidase, 6-alpha-glucosyltransferase, 3-alpha-isomaltosyltransferase and alpha-1,4-glucan lyase. All GH31 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. In most cases, the pyranose moiety recognized in subsite -1 of the substrate binding site is an alpha-D-glucose, though some GH31 family members show a preference for alpha-D-xylose. Several GH31 enzymes can accommodate both glucose and xylose and different levels of discrimination between the two have been observed. Most characterized GH31 enzymes are alpha-glucosidases. In mammals, GH31 members with alpha-glucosidase activity are implicated in at least three distinct biological processes. The lysosomal acid alpha-glucosidase (GAA) is essential for glycogen degradation and a deficiency or malfunction of this enzyme causes glycogen storage disease II, also known as Pompe disease. In the endoplasmic reticulum, alpha-glucosidase II catalyzes the second step in the N-linked oligosaccharide processing pathway that constitutes part of the quality control system for glycoprotein folding and maturation. The intestinal enzymes sucrase-isomaltase (SI) and maltase-glucoamylase (MGAM) play key roles in the final stage of carbohydrate digestion, making alpha-glucosidase inhibitors useful in the treatment of type 2 diabetes. GH31 alpha-glycosidases are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QWT17625.1	0.0	17	2072	21	2114
QNM10857.1	0.0	46	2065	50	2018
BCT46261.1	0.0	46	2060	49	2041
BBK61154.1	0.0	18	2066	18	2075
QUO30799.1	0.0	49	1393	35	1367

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6M76_A	4.05e-238	42	1054	32	963	GH31alpha-N-acetylgalactosaminidase from Enterococcus faecalis [Enterococcus faecalis ATCC 10100],6M77_A GH31 alpha-N-acetylgalactosaminidase from Enterococcus faecalis in complex with N-acetylgalactosamine [Enterococcus faecalis ATCC 10100]
7F7R_A	2.16e-237	42	1054	32	963	ChainA, GH31 alpha-N-acetylgalactosaminidase [Enterococcus faecalis ATCC 10100]
7F7Q_A	5.88e-237	42	1054	32	963	ChainA, GH31 alpha-N-acetylgalactosaminidase [Enterococcus faecalis ATCC 10100]
4LPL_A	1.01e-21	1033	1188	23	182	Structureof CBM32-1 from a family 31 glycoside hydrolase from Clostridium perfringens [Clostridium perfringens ATCC 13124]
7KMP_A	1.94e-18	507	789	636	901	ChainA, Alpha-xylosidase [Xanthomonas citri pv. citri str. 306],7KNC_A Chain A, Alpha-xylosidase [Xanthomonas citri pv. citri str. 306]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9F234	1.23e-20	65	792	48	714	Alpha-glucosidase 2 OS=Bacillus thermoamyloliquefaciens OX=1425 PE=3 SV=1
Q9FN05	8.74e-19	433	745	497	780	Probable glucan 1,3-alpha-glucosidase OS=Arabidopsis thaliana OX=3702 GN=PSL5 PE=1 SV=1
B9F676	1.50e-18	212	745	291	778	Probable glucan 1,3-alpha-glucosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0216600 PE=3 SV=1
P79403	2.35e-15	504	747	591	813	Neutral alpha-glucosidase AB OS=Sus scrofa OX=9823 GN=GANAB PE=1 SV=1
Q4R4N7	4.03e-15	504	747	591	813	Neutral alpha-glucosidase AB OS=Macaca fascicularis OX=9541 GN=GANAB PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000460	0.998593	0.000237	0.000257	0.000226	0.000187

TMHMM  Annotations     

start	end
2109	2131