CAZyme Information: MGYG000002958_00343

Basic Information

• Species: Collinsella sp900541795
• Lineage: Bacteria; Actinobacteriota; Coriobacteriia; Coriobacteriales; Coriobacteriaceae; Collinsella; Collinsella sp900541795
• CAZyme ID: MGYG000002958_00343
• CAZy Family: GH32
• CAZyme Description: Sucrose-6-phosphate hydrolase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
480	MGYG000002958_3|CGC3	53916.53	4.6324

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002958	2138240	MAG	United States	North America

• Gene Location: Start: 92401; End: 93843 Strand: +

Full Sequence      

MLNQKNKVTRADYEQWRVGQDETAARIASDPDRLLFHVEPELGWLNDPNGLVQIGDTYHI
YHQYDPFDAAHGGPVLWNHLTTKDFVTYENHGPVLFPDSDLDSSGAYSGSAFVRDGKIHY
FYTGNVKHFDRDDYDYVLTGREQNQIHMVAENPDELGEKRIAVGPVDYPDNIGTHVRDPK
ILEHDGIYYMVLGARTKDDRGCVLVYTSRDLESWGYATRIELGEKFGFMWECPDLFELDG
ELILVCCPQGVPADGWRYRNPHQCVWFPIEADWGAPSFKIVGQGMPPMVDAGFDFYAPQS
FEDAAGRRLMIGWSGCPDATAESPTVARGWQCALTVPRELSMRDGKLCQQPVHEIERMRG
DCVRATGGETVEEPGRLFDLVVSCEGAKMVELEIRKGVFVRYADGMLTLDMGGEGYGRDQ
RSIELSELRDLRVLSDTTMVEIFANGGEAALTSRTYSPAVPAMELHAKGAASMNFYRLAH

Enzyme Prediction     

EC	3.2.1.26

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH32	37	351	1.2e-83	0.9965870307167235

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd18623	GH32_ScrB-like	3.77e-141	43	344	1	289	glycoside hydrolase family 32 sucrose 6 phosphate hydrolase (sucrase). Glycosyl hydrolase family GH32 subgroup contains sucrose-6-phosphate hydrolase (sucrase, EC:3.2.1.26) among others. The enzyme cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose. These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
COG1621	SacC	1.48e-122	28	459	24	461	Sucrose-6-phosphate hydrolase SacC, GH32 family [Carbohydrate transport and metabolism].
TIGR01322	scrB_fam	7.09e-122	25	456	6	443	sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]
pfam00251	Glyco_hydro_32N	1.50e-100	37	351	1	308	Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure.
smart00640	Glyco_32	8.25e-96	37	447	1	437	Glycosyl hydrolases family 32.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QIA33880.1	0.0	25	480	2	457
AZH70195.1	0.0	1	479	1	479
QCP34478.1	7.53e-169	1	457	1	458
QMW71993.1	2.44e-162	1	457	1	458
BCD35034.1	2.44e-162	1	457	1	458

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6NU7_A	3.55e-62	25	457	25	463	Structureof sucrose-6-phosphate hydrolase from Lactobacillus gasseri [Lactobacillus gasseri 224-1],6NU8_A Structure of sucrose-6-phosphate hydrolase from Lactobacillus gasseri in complex with fructose [Lactobacillus gasseri 224-1]
7VCO_A	2.30e-56	36	456	29	458	ChainA, Sucrose-6-phosphate hydrolase [Frischella perrara],7VCP_A Chain A, Sucrose-6-phosphate hydrolase [Frischella perrara]
7BWB_A	3.35e-49	1	478	21	482	Bombyxmori GH32 beta-fructofuranosidase BmSUC1 [Bombyx mori]
7BWC_A	4.63e-48	1	478	21	482	Bombyxmori GH32 beta-fructofuranosidase BmSUC1 mutant D63A in complex with sucrose [Bombyx mori]
6NUM_A	1.44e-45	36	461	43	490	Thestructure of GH32 from Bifidobacteium adolescentis [Bifidobacterium adolescentis],6NUN_A Structure of GH32 hydrolase from Bifidobacterium adolescentis in complex with frutose [Bifidobacterium adolescentis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P07819	6.71e-87	1	474	1	472	Sucrose-6-phosphate hydrolase OS=Bacillus subtilis (strain 168) OX=224308 GN=sacA PE=3 SV=2
P37075	4.48e-80	26	459	20	440	Sucrose-6-phosphate hydrolase OS=Salmonella typhimurium OX=90371 GN=scrB PE=3 SV=1
P27217	7.35e-74	36	477	30	457	Sucrose-6-phosphate hydrolase OS=Klebsiella pneumoniae OX=573 GN=scrB PE=1 SV=3
P13394	1.17e-73	36	456	40	453	Sucrose-6-phosphate hydrolase OS=Vibrio alginolyticus OX=663 GN=scrB PE=2 SV=1
Q05936	4.17e-73	33	457	34	464	Sucrose-6-phosphate hydrolase OS=Staphylococcus xylosus OX=1288 GN=scrB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000105	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002958_00343.