CAZyme Information: MGYG000001483_00231

Basic Information

• Species: Arachnia massiliensis
• Lineage: Bacteria; Actinobacteriota; Actinomycetia; Propionibacteriales; Propionibacteriaceae; Arachnia; Arachnia massiliensis
• CAZyme ID: MGYG000001483_00231
• CAZy Family: GH35
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1859	MGYG000001483_12|CGC2	198486.86	4.2577

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001483	3211248	Isolate	not provided	not provided

• Gene Location: Start: 54435; End: 60014 Strand: -

Full Sequence      

MFNTLSRRLLAIFAVIALSITGFISPGSAQAVDPPAYQGNKITFPGNDGQPHTVTWDKNS
YMVDGERLVIWSGELHHWRVPHEDGWRDVFQKLRAAGFNAVSLYFFWGLHQSEEGGEFDF
TGPRDLDRLLTLAAEEGLYVIARPGPYVNAEISMGGLPAYFSNKSADSLRGTDPSVLEPS
KEWLAAFNDIAREHQVTDGGGSIVMYQVENELIADNEPRRAFLKELAEFVRADGITVPLF
HNDYGLGGRFSDVENLGLDFYAYDWYPLGFSCDATRGTISDQEANLRRYAPDSPNFITEA
QGGAFSPWGAPWTDNDHCKTYVDPAFTRQWGMNNIGQGVTAFNYYMIIGGTNWGYTGAPG
SGFTSYDYGAALDEDRTITPKLAVQKELGYYQRGVPQLSSMDPAPRIQPAELQGGATNVY
QRVATDDLEDSVTGNGVRFIGARLGNSNDTSTTSFAIPLNLGEEGGVITDQFTDDDRSAR
ISYSANWNQVADGTAYKGTLTRSATAGSTATVTFNGTGIEVIAPTSPQYGTATISIDGGA
PVTMDASYNTEQNAPAQQTLFSKADLAAGNHTAVITLTGQAGANANGTAFAIDAINVLGA
PSAAPTVYNNSDLDFFTFDPAPVAFRPGGTESARWTHASGHTWTAGNIDGDETYTRTAGD
SVEFTFTGTGFEVLAAFSENHGPADVFIDGVKVGQTAEEVVTTGAIPQQVIFSKRDLADG
EHTVRLVHTGEFFDGATDPRGAYFSVDAVRVYSADTAPEPEAPAGELAWPRIPQEPGTKL
TIHGRDALMLTADLKIGGVHDMLYTTSQVFDSPIVTADGTLQTLVGYSGDAGETVLRLPA
GYTVEAPASVQQTYTAATQQLRLNYTHGAAPIDITVTRADGETLILRVMDRDYAADTWMV
EGIRGDVNDKVQVSGAYLVRTVTFEGTVAHITGSMDEAGTLAVTLPAGITSYTWNGGALT
NGAAPGPVDVTEPQLDWVKKAEEMPVAVDFDDSDWVLANATTPLQHRQGPGRNGVVLDSN
RYGFHNGSVWYRAEYTAADEAPETLSFRGNAGTFGAQRKNPGFFQVWVNGEYAGARTLNG
STQSVPVPEGAITGGEQVVVTVLANNLGLNLDWSDDGLSRQNRGLYEATLGSKAGTETWK
ILGSADPFGQIDQMRGMYNTGGKIGEREGWHLPGVDTTDWAPATTMKATEPGVAWYRTEV
DLNVPEGQDTAWRLELDVDNFGNTHQTRTNGSQVDLYVNGWNIGVFIGDVGPQSSFTIPA
GFVDPMGTNEIAVAVDAKDVDQGPNMIRLVPVHSTTGAPADFEPYPAIDQAEYLEGVRTT
PVIAITSAPSELDWGTTGTITYSLTNVPAGSTLTVDAPEGWTVTPATQTTSGDTATVLVT
APASGTSGTITAKLRTPEGRTASASQAIRLTDPSVLQIDGIVAWNTAEPQEGGSNGYITA
LADGNTATHWHSRWSGGTTPFPHYVVLDLGSVQDVSSLTYIPRTGCGIPNNPPACNGTVD
GYQVFVATEGDFVSPTAEELRQVAYAEPAGVTYTKVAEGNFGGQSTGPEVVEFDEPVKAR
YVKFVSTSANSGQAWSSAAEIQLSGEATTPAAPTLGTIGAQTGEVGTAVSIQPSVTGTGP
FTWSATGLPAGVSINPTTGAITGTPTEAGTSNVTVTVEDAAGNQATASFTFTVAPAPTVD
PTEDPTVTPTVDPTDDPTTPPTQKPTTPPVKKYVRTAPYTLPGLHKGLNGRDWNTTCEPY
SQTERCRTDIWATIVVIENGQFVRKDGWTFNNLTYLPYMTREAWGANPLANTGEWTATTD
QRNWLTECDTARTGRDGCRSYTFVTVYRATAKPEGGYVFSQTNEWVFNNIVMFGGPELR

Enzyme Prediction     

No EC number prediction in MGYG000001483_00231.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH35	62	388	1.3e-86	0.9837133550488599
CBM32	1431	1582	1.4e-16	0.8951612903225806

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam01301	Glyco_hydro_35	1.52e-84	60	389	1	315	Glycosyl hydrolases family 35.
PLN03059	PLN03059	6.27e-25	53	403	29	354	beta-galactosidase; Provisional
COG1874	GanA	5.61e-22	60	256	7	171	Beta-galactosidase GanA [Carbohydrate transport and metabolism].
pfam13364	BetaGal_dom4_5	1.71e-18	1165	1281	2	111	Beta-galactosidase jelly roll domain. This domain is found in beta galactosidase enzymes. It has a jelly roll fold.
smart01029	BetaGal_dom2	5.97e-15	771	895	62	180	Beta-galactosidase, domain 2. This is the second domain of the five-domain beta-galactosidase enzyme that altogether catalyses the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and trans-glycosylation. This domain is made up of 16 antiparallel beta-strands and an alpha-helix at its C terminus. The fold of this domain appears to be unique. In addition, the last seven strands of the domain form a subdomain with an immunoglobulin-like (I-type Ig) fold in which the first strand is divided between the two beta-sheets. In penicillin spp this strand is interrupted by a 12-residue insertion which forms an additional edge-strand to the second beta-sheet of the sub-domain. The remainder of the second domain forms a series of beta-hairpins at its N terminus, four strands of which are contiguous with part of the Ig-like sub-domain, forming in total a seven-stranded antiparallel beta-sheet. This domain is associated with family Glyco_hydro_35, which is N-terminal to it, but itself has no metazoan members.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QTO38485.1	0.0	384	1637	1	1250
AOZ72117.1	0.0	29	1586	44	1568
QQM67568.1	0.0	24	1312	39	1344
VEG55570.1	0.0	21	1307	37	1340
QWW19060.1	0.0	1	1306	4	1315

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3OG2_A	5.23e-63	54	459	26	427	ChainA, Beta-galactosidase [Trichoderma reesei],3OGR_A Chain A, Beta-galactosidase [Trichoderma reesei],3OGS_A Chain A, Beta-galactosidase [Trichoderma reesei],3OGV_A Chain A, Beta-galactosidase [Trichoderma reesei]
5IFP_A	3.04e-60	54	493	46	500	StructureOf Beta-galactosidase From Aspergillus Niger [Aspergillus niger CBS 513.88]
5IFT_A	7.13e-60	54	493	46	500	STRUCTUREOF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 3-b-Galactopyranosyl glucose [Aspergillus niger CBS 513.88],5IHR_A Structure Of E298q-beta-galactosidase From Aspergillus Niger In Complex With Allolactose [Aspergillus niger CBS 513.88],5JUV_A STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 6-b-Galactopyranosyl galactose [Aspergillus niger CBS 513.88],5MGC_A STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 4-Galactosyl-lactose [Aspergillus niger CBS 513.88],5MGD_A STRUCTURE OF E298Q-BETA-GALACTOSIDASE FROM ASPERGILLUS NIGER IN COMPLEX WITH 6-Galactosyl-lactose [Aspergillus niger CBS 513.88]
1TG7_A	1.54e-58	54	396	6	354	Nativestructure of beta-galactosidase from Penicillium sp. [Penicillium sp.],1XC6_A Native Structure Of Beta-Galactosidase from Penicillium sp. in complex with Galactose [Penicillium sp.]
4IUG_A	2.02e-58	54	379	46	375	Crystalstructure of beta-galactosidase from Aspergillus oryzae in complex with galactose [Aspergillus oryzae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q4WNE4	3.07e-69	47	393	25	378	Probable beta-galactosidase C OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=lacC PE=3 SV=1
B0Y752	3.07e-69	47	393	25	378	Probable beta-galactosidase C OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=lacC PE=3 SV=1
A1DM65	1.31e-68	47	393	25	378	Probable beta-galactosidase C OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=lacC PE=3 SV=1
A1CE56	4.29e-67	39	393	17	378	Probable beta-galactosidase C OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=lacC PE=3 SV=1
A7EZS5	1.80e-66	47	393	21	374	Probable beta-galactosidase C OS=Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) OX=665079 GN=lacC PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000446	0.998451	0.000240	0.000386	0.000260	0.000189

TMHMM  Annotations     

start	end
9	31