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CAZyme Information: MGYG000000137_00265

You are here: Home > Sequence: MGYG000000137_00265

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species UBA7160 sp902363665
Lineage Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; UBA7160; UBA7160 sp902363665
CAZyme ID MGYG000000137_00265
CAZy Family GH36
CAZyme Description Bifunctional alpha-galactosidase/sucrose kinase AgaSK
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
731 82945.77 4.9686
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000000137 4141348 Isolate United Kingdom Europe
Gene Location Start: 297274;  End: 299469  Strand: -

Full Sequence      Download help

MAIQISADQR  TFTLQTKSSS  YQMAVDAYGV  LLHLYYGKKI  EAENLEYLIY  KSDVGFSGNP60
VEAGKDRTYS  LDCLPQELPS  SGVGDFREEM  IRLYHEDGSC  AADFRYEGYE  VVKGSYEIPG120
LPAVYDTEQE  TGETLIITMR  EVSSDIKVKL  YYGVMEEENV  ITRSACVCNC  GTKKVVLDQL180
LSCNLDFMFG  TYDMIYFYGR  HAMERNLERV  PVKGAKVEIG  SRRGTSSHQY  NPAVILCPPE240
TTEKNGDCYG  LCFAYSGNFT  ARAQRDQRNQ  TRVQMGIQPD  QFRFVLEGGE  TFFAPQVIMS300
YSSEGFTALS  HQYHDLIREH  ICRGPWKHAK  RPALINNWEA  TYFAFNKEKI  LKIARQAKEL360
GIEMLVLDDG  WFGKRDDDYS  GLGDWFVNEK  KLEGTLAELS  SEIHAMGMKF  GLWFEPEMVS420
EDSDLYRAHP  DWALQIPGRQ  PNRARYQLVL  DMSRKEVVDY  LYNQISDVLE  KASVDYVKWD480
MNRSVCDFYS  SKLPAERMGE  IPHRFVLGVY  DLAERLLKRF  PQLLLEGCSG  GGGRFDAGML540
YYAPQIWCSD  NTDAVNRLDI  QYGTSFFYPT  STMGAHVSAV  PNHQTGRITP  FATRGNVAMA600
GTFGYELDLN  LVTDEEKAMV  KEQLAQFDRF  YDLTHEGDYY  RLTAPKEGEL  MVWEFVAKDK660
SKAVSGVVKT  DAQGNPLPLH  VPVAGLAPDR  KYHCSYNGSV  HTGSVWNNCG  LTLVEIPKEY720
ESLLIEWTEA  E731

Enzyme Prediction      help

EC 3.2.1.22

CAZyme Signature Domains help

Created with Snap367310914618221925529232836540243847551154858462165769412712GH36
Family Start End Evalue family coverage
GH36 12 712 4e-261 0.9956395348837209

CDD Domains      download full data without filtering help

Created with Snap367310914618221925529232836540243847551154858462165769412714GalA291637Melibiase330629GH3629287Glyco_hydro_36N651726Glyco_hydro_36C
Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
COG3345 GalA 0.0 12 714 4 668
Alpha-galactosidase [Carbohydrate transport and metabolism].
pfam02065 Melibiase 0.0 291 637 1 347
Melibiase. Glycoside hydrolase families GH27, GH31 and GH36 form the glycoside hydrolase clan GH-D. Glycoside hydrolase family 36 can be split into 11 families, GH36A to GH36K. This family includes enzymes from GH36A-B and GH36D-K and from GH27.
cd14791 GH36 8.05e-146 330 629 1 299
glycosyl hydrolase family 36 (GH36). GH36 enzymes occur in prokaryotes, eukaryotes, and archaea with a wide range of hydrolytic activities, including alpha-galactosidase, alpha-N-acetylgalactosaminidase, stachyose synthase, and raffinose synthase. All GH36 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH36 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.
pfam16875 Glyco_hydro_36N 1.47e-101 29 287 1 256
Glycosyl hydrolase family 36 N-terminal domain. This domain is found at the N-terminus of many family 36 glycoside hydrolases. It has a beta-supersandwich fold.
pfam16874 Glyco_hydro_36C 1.26e-15 651 726 2 77
Glycosyl hydrolase family 36 C-terminal domain. This domain is found at the C-terminus of many family 36 glycoside hydrolases. It has a beta-sandwich structure with a Greek key motif.

CAZyme Hits      help

Created with Snap36731091461822192552923283654024384755115485846216576941722BCK01162.1|GH361722QBE96096.1|GH361722QMW81127.1|GH361722QIB56139.1|GH361722QIX93423.1|GH36
Hit ID E-Value Query Start Query End Hit Start Hit End
BCK01162.1 2.25e-303 1 722 1 719
QBE96096.1 8.43e-294 1 722 1 721
QMW81127.1 1.59e-291 1 722 1 721
QIB56139.1 1.59e-291 1 722 1 721
QIX93423.1 9.47e-289 1 722 1 725

PDB Hits      download full data without filtering help

Created with Snap367310914618221925529232836540243847551154858462165769417292YFN_A17244FNQ_A17244FNR_A17244FNT_A17244FNP_A
Hit ID E-Value Query Start Query End Hit Start Hit End Description
2YFN_A 6.71e-287 1 729 1 719
galactosidasedomain of alpha-galactosidase-sucrose kinase, AgaSK [[Ruminococcus] gnavus E1],2YFO_A GALACTOSIDASE DOMAIN OF ALPHA-GALACTOSIDASE-SUCROSE KINASE, AGASK, in complex with galactose [[Ruminococcus] gnavus E1]
4FNQ_A 6.64e-223 1 724 1 721
Crystalstructure of GH36 alpha-galactosidase AgaB from Geobacillus stearothermophilus [Geobacillus stearothermophilus]
4FNR_A 4.90e-220 1 724 1 721
Crystalstructure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_B Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_C Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_D Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus]
4FNT_A 2.24e-218 1 724 1 721
Crystalstructure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus],4FNT_B Crystal structure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus],4FNT_C Crystal structure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus],4FNT_D Crystal structure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus]
4FNP_A 3.17e-218 1 724 1 721
Crystalstructure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNP_B Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNP_C Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNP_D Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNS_A Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus in complex with 1-deoxygalactonojirimycin [Geobacillus stearothermophilus],4FNS_B Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus in complex with 1-deoxygalactonojirimycin [Geobacillus stearothermophilus],4FNS_C Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus in complex with 1-deoxygalactonojirimycin [Geobacillus stearothermophilus],4FNS_D Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus in complex with 1-deoxygalactonojirimycin [Geobacillus stearothermophilus]

Swiss-Prot Hits      download full data without filtering help

Created with Snap36731091461822192552923283654024384755115485846216576941729sp|G4T4R7|AGASK_RUMGN1724sp|Q9ALJ4|AGAA_GEOSE3692sp|G1UB44|MELA_LACAC5692sp|P43467|AGAL1_PEDPE12718sp|P27756|AGAL_STRMU
Hit ID E-Value Query Start Query End Hit Start Hit End Description
G4T4R7 6.00e-283 1 729 1 719
Bifunctional alpha-galactosidase/sucrose kinase AgaSK OS=Ruminococcus gnavus OX=33038 GN=agaSK PE=1 SV=1
Q9ALJ4 2.68e-219 1 724 1 721
Alpha-galactosidase AgaA OS=Geobacillus stearothermophilus OX=1422 GN=agaA PE=1 SV=1
G1UB44 2.67e-209 3 692 6 693
Alpha-galactosidase Mel36A OS=Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) OX=272621 GN=melA PE=1 SV=1
P43467 3.23e-205 5 692 4 692
Alpha-galactosidase 1 OS=Pediococcus pentosaceus OX=1255 GN=agaR PE=3 SV=1
P27756 1.43e-174 12 718 10 707
Alpha-galactosidase OS=Streptococcus mutans serotype c (strain ATCC 700610 / UA159) OX=210007 GN=aga PE=3 SV=3

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
1.000051 0.000001 0.000000 0.000000 0.000000 0.000000

TMHMM  Annotations      help

There is no transmembrane helices in MGYG000000137_00265.