logo
sublogo
You are browsing environment: HUMAN GUT
help

CAZyme Information: MGYG000000969_00382

You are here: Home > Sequence: MGYG000000969_00382

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species Eubacterium_I sp900546495
Lineage Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Eubacterium_I; Eubacterium_I sp900546495
CAZyme ID MGYG000000969_00382
CAZy Family GH36
CAZyme Description Bifunctional alpha-galactosidase/sucrose kinase AgaSK
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
932 MGYG000000969_3|CGC1 104868.38 4.7731
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000000969 2650866 MAG Spain Europe
Gene Location Start: 86132;  End: 88930  Strand: -

Full Sequence      Download help

MAIIFDANRK  IFTIHTKHTT  YQMQADAKGY  LLHLYYGVRV  KGTMDYLLCY  ADHGFSGNPY60
AAGMDRTYSL  DALPQEYPSL  GTGDCRNIAL  NITSAVGTEC  CDPIFNSYKI  TKGKYSLQGL120
PAVWAADDEA  ETLEIVLKDD  LTQVEIHLLY  GVLEDADIIT  RSVVIKNTGT  ETITVKKALS180
ACLDFVQGDY  DAISFYGRHA  MERNLERVPV  GHGTYQIGSR  RGSSSHQYNP  GVILADRTAT240
EEAGNCYGML  FMYSGNFVCE  AERDQFNQTR  FQMGLGDELF  AYPVAAGAEF  TTPEVIMTYS300
DQGFAKLSRQ  YHNCILNHVC  KGRQVHTNRP  ILINSWEAAY  FDFDGDTIVD  LAKQAAELGI360
DMVVMDDGWF  GKRNDDNSSL  GDWFVNEKKL  GGTLGQLIER  VNAQGVKFGI  WIEPEMVNED420
SDLYREHPDW  ALTIPGRMPI  RSRNQLLLDF  SRKEVREEIL  KRICAILDQG  NIEYIKWDMN480
RSMSDVYAGN  VPYDYVLGLY  DFLEKLTSRY  PDILIEGCSG  GGGRFDAGMM  YYTPQIWCSD540
NTDAINRTRI  QYGTSFFYPT  AVVGSHVSAV  PNHQTGRITS  LNTRGVVAMA  GTFGYEMNPA600
LLSSEEKEEI  RTQLATYRRH  QELIREGDYY  RLSDPFKEDV  AAWMSVAKDQ  SQALVSVVRL660
SAEGNPFGTY  VKLKGLDAEC  FYLEETTGKV  YSGMALMQAG  ILLPMAVIEY  EAYQFSFKKM720
QEATELYDLL  RKKIGAERKV  ISIFGGSGSG  KTTMAEILQQ  QFLADGIGCF  IVHGDDYPHR780
IPKCNDQERE  LIYQKSGETG  LNAYLGTPQE  IEYDRINQVL  AKFHVGDTEI  ELKKMGREDD840
EIWYEQTDLT  GVQVLLLEWT  HGGSEYLNGV  DISVYLDSTP  EETKARRIRR  GRDENAASAF900
IQLVLSLEAQ  KLEQQAKQAD  LIVGKDGRVY  ES932

Enzyme Prediction      help

EC 3.2.1.22

CAZyme Signature Domains help

Created with Snap469313918623327932637241946651255960565269974579283888511701GH36
Family Start End Evalue family coverage
GH36 11 701 1.7e-261 0.9956395348837209

CDD Domains      download full data without filtering help

Created with Snap469313918623327932637241946651255960565269974579283888512720GalA290627Melibiase329619GH3629285Glyco_hydro_36N640717Glyco_hydro_36C
Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
COG3345 GalA 0.0 12 720 4 687
Alpha-galactosidase [Carbohydrate transport and metabolism].
pfam02065 Melibiase 9.55e-179 290 627 2 347
Melibiase. Glycoside hydrolase families GH27, GH31 and GH36 form the glycoside hydrolase clan GH-D. Glycoside hydrolase family 36 can be split into 11 families, GH36A to GH36K. This family includes enzymes from GH36A-B and GH36D-K and from GH27.
cd14791 GH36 3.67e-141 329 619 2 299
glycosyl hydrolase family 36 (GH36). GH36 enzymes occur in prokaryotes, eukaryotes, and archaea with a wide range of hydrolytic activities, including alpha-galactosidase, alpha-N-acetylgalactosaminidase, stachyose synthase, and raffinose synthase. All GH36 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH36 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.
pfam16875 Glyco_hydro_36N 5.81e-107 29 285 1 256
Glycosyl hydrolase family 36 N-terminal domain. This domain is found at the N-terminus of many family 36 glycoside hydrolases. It has a beta-supersandwich fold.
pfam16874 Glyco_hydro_36C 6.13e-21 640 717 1 78
Glycosyl hydrolase family 36 C-terminal domain. This domain is found at the C-terminus of many family 36 glycoside hydrolases. It has a beta-sandwich structure with a Greek key motif.

CAZyme Hits      help

Created with Snap46931391862332793263724194665125596056526997457928388851931ACR74280.1|GH361931CBK89379.1|GH361931CBK94329.1|GH361931AWY97922.1|GH361931CCA61959.1|GH36|3.2.1.22
Hit ID E-Value Query Start Query End Hit Start Hit End
ACR74280.1 0.0 1 931 7 951
CBK89379.1 0.0 1 931 1 945
CBK94329.1 0.0 1 931 1 945
AWY97922.1 0.0 1 931 1 934
CCA61959.1 0.0 1 931 1 934

PDB Hits      download full data without filtering help

Created with Snap469313918623327932637241946651255960565269974579283888517202YFN_A17224FNQ_A37182XN0_A37182XN2_A17224FNR_A
Hit ID E-Value Query Start Query End Hit Start Hit End Description
2YFN_A 0.0 1 720 1 720
galactosidasedomain of alpha-galactosidase-sucrose kinase, AgaSK [[Ruminococcus] gnavus E1],2YFO_A GALACTOSIDASE DOMAIN OF ALPHA-GALACTOSIDASE-SUCROSE KINASE, AGASK, in complex with galactose [[Ruminococcus] gnavus E1]
4FNQ_A 3.84e-217 1 722 1 729
Crystalstructure of GH36 alpha-galactosidase AgaB from Geobacillus stearothermophilus [Geobacillus stearothermophilus]
2XN0_A 6.00e-217 3 718 6 729
Structureof alpha-galactosidase from Lactobacillus acidophilus NCFM, PtCl4 derivative [Lactobacillus acidophilus NCFM],2XN0_B Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM, PtCl4 derivative [Lactobacillus acidophilus NCFM],2XN1_A Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM with TRIS [Lactobacillus acidophilus NCFM],2XN1_B Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM with TRIS [Lactobacillus acidophilus NCFM],2XN1_C Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM with TRIS [Lactobacillus acidophilus NCFM],2XN1_D Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM with TRIS [Lactobacillus acidophilus NCFM]
2XN2_A 1.69e-216 3 718 6 729
Structureof alpha-galactosidase from Lactobacillus acidophilus NCFM with galactose [Lactobacillus acidophilus NCFM]
4FNR_A 8.65e-216 1 722 1 729
Crystalstructure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_B Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_C Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_D Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus]

Swiss-Prot Hits      download full data without filtering help

Created with Snap46931391862332793263724194665125596056526997457928388851931sp|G4T4R7|AGASK_RUMGN3718sp|G1UB44|MELA_LACAC1722sp|Q9ALJ4|AGAA_GEOSE3722sp|P43467|AGAL1_PEDPE1719sp|P27756|AGAL_STRMU
Hit ID E-Value Query Start Query End Hit Start Hit End Description
G4T4R7 0.0 1 931 1 934
Bifunctional alpha-galactosidase/sucrose kinase AgaSK OS=Ruminococcus gnavus OX=33038 GN=agaSK PE=1 SV=1
G1UB44 3.28e-216 3 718 6 729
Alpha-galactosidase Mel36A OS=Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) OX=272621 GN=melA PE=1 SV=1
Q9ALJ4 4.73e-215 1 722 1 729
Alpha-galactosidase AgaA OS=Geobacillus stearothermophilus OX=1422 GN=agaA PE=1 SV=1
P43467 2.77e-206 3 722 4 732
Alpha-galactosidase 1 OS=Pediococcus pentosaceus OX=1255 GN=agaR PE=3 SV=1
P27756 8.58e-173 1 719 1 719
Alpha-galactosidase OS=Streptococcus mutans serotype c (strain ATCC 700610 / UA159) OX=210007 GN=aga PE=3 SV=3

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
1.000067 0.000000 0.000000 0.000000 0.000000 0.000000

TMHMM  Annotations      help

There is no transmembrane helices in MGYG000000969_00382.