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CAZyme Information: MGYG000003377_00164

You are here: Home > Sequence: MGYG000003377_00164

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species Phytobacter sp002377245
Lineage Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Phytobacter; Phytobacter sp002377245
CAZyme ID MGYG000003377_00164
CAZy Family GH36
CAZyme Description Alpha-galactosidase
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
707 MGYG000003377_6|CGC1 80253.65 6.242
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000003377 5122475 MAG United States North America
Gene Location Start: 40195;  End: 42318  Strand: +

Full Sequence      Download help

METRYLRLES  ATTDVIIKTH  PFAEIIYWGP  HLQHFSAQDA  QSLIRPVANG  RLDVDSPVTL60
MAESGHGLFG  SPGLEGHRNG  LDGSPIFTTV  DVQHNGQSLT  LISEDEVAGL  RLTSELHLDV120
SGVLQVRHGL  TNLADSAWQV  NRLAVTLPVA  EYARDVMAFH  GRWIREFQPH  RVTLEHDSFV180
IENRRGRTSH  EHFPALMTGS  RNFSESQGSV  WGVHLGWSGN  HRLRAEVKTD  GRRYVQAEAL240
YLPGEMALAE  GETLWTPWLY  ASHSSQGLNG  MSQQFHRFLR  ANVIRFVENK  PRPVHLNTWE300
GIYFDHNPAY  IMKMADDAAA  LGVERFIIDD  GWYKGRNDDF  AALGDWYLDE  GKYPDGLRPV360
IDHVKALGME  FGIWVEPEMI  SPNSDLYRAH  PDWVLALEGY  SQILGRHQLV  LNITLPEAFD420
YLLARMSWLL  GEHEIDYVKW  DMNRELVQPG  QGRRAAADAQ  THAFYRLLDT  LRSRFPHIEF480
ESCSAGGGRI  DYEVLTRCHR  FWASDNNDAL  ERNIIQRGMG  YFFPPEVMGA  HIGNHRCHAT540
YRQHSIEFRG  LTALFGHLGL  ELDPVNADAQ  EREGYRHYAA  LYKQWREVIH  SGTQWRIDMP600
DSTTLVQGIV  SQDKRQALYI  VSQLAMPDYT  LLAPLRVAGL  EAQARYRITL  LDHPNIRITD660
EGGHTMRQLP  AWMTAPQIAS  GEWLMQAGLA  LPVLDPESAI  LIGFERV707

Enzyme Prediction      help

EC 3.2.1.22

CAZyme Signature Domains help

Created with Snap35701061411762122472823183533884244594945305656006366716651GH36
Family Start End Evalue family coverage
GH36 6 651 3.1e-237 0.9738372093023255

CDD Domains      download full data without filtering help

Created with Snap35701061411762122472823183533884244594945305656006366715693GalA253592Melibiase291584GH3624237Glyco_hydro_36N297373GH27
Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
COG3345 GalA 0.0 5 693 3 687
Alpha-galactosidase [Carbohydrate transport and metabolism].
pfam02065 Melibiase 6.87e-137 253 592 1 347
Melibiase. Glycoside hydrolase families GH27, GH31 and GH36 form the glycoside hydrolase clan GH-D. Glycoside hydrolase family 36 can be split into 11 families, GH36A to GH36K. This family includes enzymes from GH36A-B and GH36D-K and from GH27.
cd14791 GH36 2.76e-126 291 584 1 299
glycosyl hydrolase family 36 (GH36). GH36 enzymes occur in prokaryotes, eukaryotes, and archaea with a wide range of hydrolytic activities, including alpha-galactosidase, alpha-N-acetylgalactosaminidase, stachyose synthase, and raffinose synthase. All GH36 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH36 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.
pfam16875 Glyco_hydro_36N 5.38e-46 24 237 4 244
Glycosyl hydrolase family 36 N-terminal domain. This domain is found at the N-terminus of many family 36 glycoside hydrolases. It has a beta-supersandwich fold.
cd14792 GH27 2.01e-11 297 373 6 84
glycosyl hydrolase family 27 (GH27). GH27 enzymes occur in eukaryotes, prokaryotes, and archaea with a wide range of hydrolytic activities, including alpha-glucosidase (glucoamylase and sucrase-isomaltase), alpha-N-acetylgalactosaminidase, and 3-alpha-isomalto-dextranase. All GH27 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH27 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.

CAZyme Hits      help

Created with Snap35701061411762122472823183533884244594945305656006366711707QLU01836.1|GH361707AOQ01982.1|GH361707QBA38267.1|GH361707QLU74229.1|GH361707QCC95061.1|GH36
Hit ID E-Value Query Start Query End Hit Start Hit End
QLU01836.1 0.0 1 707 1 707
AOQ01982.1 0.0 1 707 1 707
QBA38267.1 0.0 1 707 1 707
QLU74229.1 0.0 1 707 1 707
QCC95061.1 0.0 1 707 1 707

PDB Hits      download full data without filtering help

Created with Snap3570106141176212247282318353388424459494530565600636671326924FNR_A336924FNQ_A326924FNU_A326484FNT_A326484FNP_A
Hit ID E-Value Query Start Query End Hit Start Hit End Description
4FNR_A 1.55e-106 32 692 73 711
Crystalstructure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_B Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_C Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_D Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus]
4FNQ_A 3.22e-105 33 692 74 711
Crystalstructure of GH36 alpha-galactosidase AgaB from Geobacillus stearothermophilus [Geobacillus stearothermophilus]
4FNU_A 1.24e-104 32 692 73 711
Crystalstructure of GH36 alpha-galactosidase AgaA A355E D478A from Geobacillus stearothermophilus in complex with stachyose [Geobacillus stearothermophilus],4FNU_B Crystal structure of GH36 alpha-galactosidase AgaA A355E D478A from Geobacillus stearothermophilus in complex with stachyose [Geobacillus stearothermophilus],4FNU_C Crystal structure of GH36 alpha-galactosidase AgaA A355E D478A from Geobacillus stearothermophilus in complex with stachyose [Geobacillus stearothermophilus],4FNU_D Crystal structure of GH36 alpha-galactosidase AgaA A355E D478A from Geobacillus stearothermophilus in complex with stachyose [Geobacillus stearothermophilus]
4FNT_A 1.74e-104 32 648 73 692
Crystalstructure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus],4FNT_B Crystal structure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus],4FNT_C Crystal structure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus],4FNT_D Crystal structure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus]
4FNP_A 2.43e-104 32 648 73 692
Crystalstructure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNP_B Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNP_C Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNP_D Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNS_A Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus in complex with 1-deoxygalactonojirimycin [Geobacillus stearothermophilus],4FNS_B Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus in complex with 1-deoxygalactonojirimycin [Geobacillus stearothermophilus],4FNS_C Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus in complex with 1-deoxygalactonojirimycin [Geobacillus stearothermophilus],4FNS_D Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus in complex with 1-deoxygalactonojirimycin [Geobacillus stearothermophilus]

Swiss-Prot Hits      download full data without filtering help

Created with Snap35701061411762122472823183533884244594945305656006366711706sp|P16551|RAFA_ECOLX32692sp|Q9ALJ4|AGAA_GEOSE155648sp|B8NWY6|AGALC_ASPFN155648sp|Q2TW69|AGALC_ASPOR63697sp|G4T4R7|AGASK_RUMGN
Hit ID E-Value Query Start Query End Hit Start Hit End Description
P16551 0.0 1 706 1 706
Alpha-galactosidase OS=Escherichia coli OX=562 GN=rafA PE=1 SV=1
Q9ALJ4 8.48e-106 32 692 73 711
Alpha-galactosidase AgaA OS=Geobacillus stearothermophilus OX=1422 GN=agaA PE=1 SV=1
B8NWY6 3.85e-99 155 648 213 717
Probable alpha-galactosidase C OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=aglC PE=3 SV=2
Q2TW69 3.85e-99 155 648 213 717
Probable alpha-galactosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=aglC PE=3 SV=1
G4T4R7 6.34e-99 63 697 99 709
Bifunctional alpha-galactosidase/sucrose kinase AgaSK OS=Ruminococcus gnavus OX=33038 GN=agaSK PE=1 SV=1

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
1.000050 0.000000 0.000000 0.000000 0.000000 0.000000

TMHMM  Annotations      help

There is no transmembrane helices in MGYG000003377_00164.