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CAZyme Information: MGYG000003457_00062

You are here: Home > Sequence: MGYG000003457_00062

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species UBA9732 sp001940825
Lineage Bacteria; Spirochaetota; Spirochaetia; Sphaerochaetales; Sphaerochaetaceae; UBA9732; UBA9732 sp001940825
CAZyme ID MGYG000003457_00062
CAZy Family GH36
CAZyme Description Alpha-galactosidase AgaA
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
792 MGYG000003457_2|CGC1 89630.97 6.7442
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000003457 1983561 MAG Fiji Oceania
Gene Location Start: 2095;  End: 4473  Strand: +

Full Sequence      Download help

MAERQHMIRL  DGKGTTYIMR  INEAGHLENL  YYGKKLRQDA  MVDALLPKRE  APIGIGAGYS60
EASPLLYLET  ACLETSTPGK  GDYRSPAVVV  EYSKGMQTLD  FVYKAHRILK  GKPSRFENFA120
QSYADEDEAT  TIEIQLEDKM  LPIMLQLNYT  VFPNCDTMVR  SATITNKTAT  TVLIKNIASL180
QLDIPSAQWN  VVSFDGAWAR  ERHITRQVLR  SGKLEFSSTV  GVSGAAHNPC  IFLESPDCTA240
NRGSCYAFNL  IYSGDHREVV  ELSPYGITRF  QTGINPQSFT  WKLLPNASFT  TPEAVMTYSC300
LGNNGASANL  HEFVNNHIVR  GHWKFRERPI  LCNNWEATYF  NFNEKKLLDL  AKAAKDLGIE360
LFVLDDGWFG  TRDDDTTSLG  DWIVNAKKLP  SGITRLSEKI  HEMGLLFGIW  VEPEMISRKS420
LLFDKHPDWM  VAIPGRKPSV  GRNQFILDLT  RKEVRDYIFE  ALSEVFNLGM  VDYVKWDMNR480
TFTDMYSTLF  TSMTEFNYRY  VSGLYELLGR  LTAKFPKILF  EGCSSGGNRY  DLGMLCYMPQ540
IWTSDNTDAY  CRTLIQGGTS  YGYPISAMGA  HVSESPNHQT  LRRSDIESRF  NVAAFGAFGY600
ELDLTALNAQ  QKATVKAQIE  FYKKHRALLQ  FGRFIRLEEN  SNTDNSVIAH  SSGKTRWAVV660
NDDLSEMLVL  DFQTLNEPNP  GQDTLRIPFA  RPDFDYEVSP  RLQRISLDVF  GSLINMLAPV720
NLSGESRLKQ  MLLDNYTLKS  EDEHYIVSGD  VLAYGGLHLD  PQFGGTGYNN  KVRILGDFGS780
RLYYIVRIKK  SR792

Enzyme Prediction      help

EC 3.2.1.22

CAZyme Signature Domains help

Created with Snap39791181581982372773163563964354755145545946336737127528704GH36
Family Start End Evalue family coverage
GH36 8 704 2.1e-243 0.9781976744186046

CDD Domains      download full data without filtering help

Created with Snap39791181581982372773163563964354755145545946336737127525698GalA288632Melibiase327624GH3625283Glyco_hydro_36N333409GH27
Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
COG3345 GalA 8.90e-179 5 698 1 648
Alpha-galactosidase [Carbohydrate transport and metabolism].
pfam02065 Melibiase 7.75e-175 288 632 1 347
Melibiase. Glycoside hydrolase families GH27, GH31 and GH36 form the glycoside hydrolase clan GH-D. Glycoside hydrolase family 36 can be split into 11 families, GH36A to GH36K. This family includes enzymes from GH36A-B and GH36D-K and from GH27.
cd14791 GH36 3.34e-139 327 624 1 299
glycosyl hydrolase family 36 (GH36). GH36 enzymes occur in prokaryotes, eukaryotes, and archaea with a wide range of hydrolytic activities, including alpha-galactosidase, alpha-N-acetylgalactosaminidase, stachyose synthase, and raffinose synthase. All GH36 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH36 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.
pfam16875 Glyco_hydro_36N 1.19e-90 25 283 1 255
Glycosyl hydrolase family 36 N-terminal domain. This domain is found at the N-terminus of many family 36 glycoside hydrolases. It has a beta-supersandwich fold.
cd14792 GH27 9.53e-16 333 409 6 84
glycosyl hydrolase family 27 (GH27). GH27 enzymes occur in eukaryotes, prokaryotes, and archaea with a wide range of hydrolytic activities, including alpha-glucosidase (glucoamylase and sucrase-isomaltase), alpha-N-acetylgalactosaminidase, and 3-alpha-isomalto-dextranase. All GH27 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH27 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.

CAZyme Hits      help

Created with Snap39791181581982372773163563964354755145545946336737127521788AEV29196.1|GH361788ADY12418.1|GH361789BCN30096.1|GH3610785QEN07610.1|GH3610789QUH31721.1|GH36
Hit ID E-Value Query Start Query End Hit Start Hit End
AEV29196.1 0.0 1 788 2 784
ADY12418.1 0.0 1 788 2 779
BCN30096.1 1.68e-247 1 789 2 796
QEN07610.1 5.10e-239 10 785 25 793
QUH31721.1 1.01e-236 10 789 12 786

PDB Hits      download full data without filtering help

Created with Snap3979118158198237277316356396435475514554594633673712752166984FNQ_A156993MI6_A166984FNR_A166984FNT_A166984FNP_A
Hit ID E-Value Query Start Query End Hit Start Hit End Description
4FNQ_A 1.53e-190 16 698 20 692
Crystalstructure of GH36 alpha-galactosidase AgaB from Geobacillus stearothermophilus [Geobacillus stearothermophilus]
3MI6_A 2.55e-190 15 699 19 696
ChainA, Alpha-galactosidase [Levilactobacillus brevis ATCC 367],3MI6_B Chain B, Alpha-galactosidase [Levilactobacillus brevis ATCC 367],3MI6_C Chain C, Alpha-galactosidase [Levilactobacillus brevis ATCC 367],3MI6_D Chain D, Alpha-galactosidase [Levilactobacillus brevis ATCC 367]
4FNR_A 3.06e-190 16 698 20 692
Crystalstructure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_B Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_C Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_D Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus]
4FNT_A 9.72e-189 16 698 20 692
Crystalstructure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus],4FNT_B Crystal structure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus],4FNT_C Crystal structure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus],4FNT_D Crystal structure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus]
4FNP_A 1.37e-188 16 698 20 692
Crystalstructure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNP_B Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNP_C Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNP_D Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNS_A Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus in complex with 1-deoxygalactonojirimycin [Geobacillus stearothermophilus],4FNS_B Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus in complex with 1-deoxygalactonojirimycin [Geobacillus stearothermophilus],4FNS_C Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus in complex with 1-deoxygalactonojirimycin [Geobacillus stearothermophilus],4FNS_D Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus in complex with 1-deoxygalactonojirimycin [Geobacillus stearothermophilus]

Swiss-Prot Hits      download full data without filtering help

Created with Snap397911815819823727731635639643547551455459463367371275216698sp|Q9ALJ4|AGAA_GEOSE3697sp|P43467|AGAL1_PEDPE3697sp|G1UB44|MELA_LACAC15696sp|G4T4R7|AGASK_RUMGN79637sp|P27756|AGAL_STRMU
Hit ID E-Value Query Start Query End Hit Start Hit End Description
Q9ALJ4 1.68e-189 16 698 20 692
Alpha-galactosidase AgaA OS=Geobacillus stearothermophilus OX=1422 GN=agaA PE=1 SV=1
P43467 3.77e-174 3 697 8 693
Alpha-galactosidase 1 OS=Pediococcus pentosaceus OX=1255 GN=agaR PE=3 SV=1
G1UB44 7.85e-167 3 697 10 694
Alpha-galactosidase Mel36A OS=Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) OX=272621 GN=melA PE=1 SV=1
G4T4R7 3.31e-162 15 696 19 682
Bifunctional alpha-galactosidase/sucrose kinase AgaSK OS=Ruminococcus gnavus OX=33038 GN=agaSK PE=1 SV=1
P27756 1.55e-142 79 637 77 633
Alpha-galactosidase OS=Streptococcus mutans serotype c (strain ATCC 700610 / UA159) OX=210007 GN=aga PE=3 SV=3

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
1.000048 0.000000 0.000000 0.000000 0.000000 0.000000

TMHMM  Annotations      help

There is no transmembrane helices in MGYG000003457_00062.