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CAZyme Information: MGYG000004124_00251

You are here: Home > Sequence: MGYG000004124_00251

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species
Lineage Bacteria; Firmicutes_A; Clostridia; Oscillospirales; QAKW01; QAKW01;
CAZyme ID MGYG000004124_00251
CAZy Family GH36
CAZyme Description Bifunctional alpha-galactosidase/sucrose kinase AgaSK
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
734 MGYG000004124_2|CGC1 83755.38 5.9444
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000004124 1823169 MAG United Kingdom Europe
Gene Location Start: 104710;  End: 106914  Strand: +

Full Sequence      Download help

MSIQFFPETK  TFRLETKETS  YIMSVSKPGH  LLHLYYGDKL  GNDDISVLTA  DERRTGFSPA60
PYDFSDSRYS  LDCMPQEFST  DGVGDFRYTS  ISIVNADGSY  AFDGKYKAHH  IYSGKYSLKG120
LPSLFANPDE  RSDTLEVELY  DYSTGVSVTL  LYGVFEDKDV  ITRSVIVKNG  GNGTVKLRRI180
MSMNLDFRRD  GFDFIHFYGH  AYLERLTERE  HVTHSACEIG  SVKGTPSHEH  NPSVVLCDRN240
ADEDHGECYG  AALLYTGNFV  CNAKVDQLCQ  TRFVMGINPE  KFEFILNTDD  TFTAPEVVMS300
YSAHGFAKMT  HHLHDVFREN  ACRSKFMKQR  RPVLVNNWEA  TRFSFTGEKI  VNIAHEGKKL360
GADLFVLDDG  WFGARNDEHA  GLGDWTVNRK  KMGGDLSDII  KQVNDDGLMF  GLWFEPEMIN420
EDSDLYRAHP  DWALKIPGRA  PALCRNQLVL  DITRKDVRNY  IIKAVNDILD  NNHIEYVKWD480
FNRYLTDAYS  ALCDKEHQGE  IFHRFVLGFY  ELLDGIILTH  PDILFEGCSG  GGSRFDGGML540
CYQPQIWCSD  DTDAVWRTKI  QYGTSFAYPV  SSMGAHVSAC  PNKKTKRTVS  FKTRAAVAMS600
GTFGYELDTT  KMTDEEKTEC  AEYTALFRKY  YDVITYGDYY  RLTNPFENTR  YVAWEHAAKD660
KSECLITAVT  MDAYTQHHVM  YIKPKGLDEN  AMYETELENG  THIKMSGKTL  MKVGLPFDLF720
VSQYESHQFY  LKKV734

Enzyme Prediction      help

EC 3.2.1.22

CAZyme Signature Domains help

Created with Snap367311014618322025629333036740344047751355058762366069712715GH36
Family Start End Evalue family coverage
GH36 12 715 2.2e-242 0.9941860465116279

CDD Domains      download full data without filtering help

Created with Snap3673110146183220256293330367403440477513550587623660697291637Melibiase12715GalA330629GH3629287Glyco_hydro_36N651731Glyco_hydro_36C
Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
pfam02065 Melibiase 9.22e-174 291 637 1 347
Melibiase. Glycoside hydrolase families GH27, GH31 and GH36 form the glycoside hydrolase clan GH-D. Glycoside hydrolase family 36 can be split into 11 families, GH36A to GH36K. This family includes enzymes from GH36A-B and GH36D-K and from GH27.
COG3345 GalA 2.69e-165 12 715 4 665
Alpha-galactosidase [Carbohydrate transport and metabolism].
cd14791 GH36 1.22e-129 330 629 1 299
glycosyl hydrolase family 36 (GH36). GH36 enzymes occur in prokaryotes, eukaryotes, and archaea with a wide range of hydrolytic activities, including alpha-galactosidase, alpha-N-acetylgalactosaminidase, stachyose synthase, and raffinose synthase. All GH36 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH36 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.
pfam16875 Glyco_hydro_36N 3.79e-91 29 287 1 256
Glycosyl hydrolase family 36 N-terminal domain. This domain is found at the N-terminus of many family 36 glycoside hydrolases. It has a beta-supersandwich fold.
pfam16874 Glyco_hydro_36C 2.70e-17 651 731 1 78
Glycosyl hydrolase family 36 C-terminal domain. This domain is found at the C-terminus of many family 36 glycoside hydrolases. It has a beta-sandwich structure with a Greek key motif.

CAZyme Hits      help

Created with Snap36731101461832202562933303674034404775135505876236606971714BCN32673.1|GH366731AWY99332.1|GH3610733QJU16478.1|GH3610733ASU30552.1|GH3610733ANU77746.1|GH36
Hit ID E-Value Query Start Query End Hit Start Hit End
BCN32673.1 7.97e-258 1 714 1 711
AWY99332.1 3.21e-256 6 731 4 723
QJU16478.1 3.25e-249 10 733 10 728
ASU30552.1 4.61e-249 10 733 10 728
ANU77746.1 4.61e-249 10 733 10 728

PDB Hits      download full data without filtering help

Created with Snap367311014618322025629333036740344047751355058762366069717292YFN_A17344FNR_A17344FNP_A17344FNQ_A17344FNU_A
Hit ID E-Value Query Start Query End Hit Start Hit End Description
2YFN_A 2.63e-239 1 729 1 715
galactosidasedomain of alpha-galactosidase-sucrose kinase, AgaSK [[Ruminococcus] gnavus E1],2YFO_A GALACTOSIDASE DOMAIN OF ALPHA-GALACTOSIDASE-SUCROSE KINASE, AGASK, in complex with galactose [[Ruminococcus] gnavus E1]
4FNR_A 9.80e-210 1 734 1 727
Crystalstructure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_B Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_C Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_D Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus]
4FNP_A 1.57e-208 1 734 1 727
Crystalstructure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNP_B Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNP_C Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNP_D Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNS_A Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus in complex with 1-deoxygalactonojirimycin [Geobacillus stearothermophilus],4FNS_B Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus in complex with 1-deoxygalactonojirimycin [Geobacillus stearothermophilus],4FNS_C Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus in complex with 1-deoxygalactonojirimycin [Geobacillus stearothermophilus],4FNS_D Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus in complex with 1-deoxygalactonojirimycin [Geobacillus stearothermophilus]
4FNQ_A 2.23e-208 1 734 1 727
Crystalstructure of GH36 alpha-galactosidase AgaB from Geobacillus stearothermophilus [Geobacillus stearothermophilus]
4FNU_A 3.15e-208 1 734 1 727
Crystalstructure of GH36 alpha-galactosidase AgaA A355E D478A from Geobacillus stearothermophilus in complex with stachyose [Geobacillus stearothermophilus],4FNU_B Crystal structure of GH36 alpha-galactosidase AgaA A355E D478A from Geobacillus stearothermophilus in complex with stachyose [Geobacillus stearothermophilus],4FNU_C Crystal structure of GH36 alpha-galactosidase AgaA A355E D478A from Geobacillus stearothermophilus in complex with stachyose [Geobacillus stearothermophilus],4FNU_D Crystal structure of GH36 alpha-galactosidase AgaA A355E D478A from Geobacillus stearothermophilus in complex with stachyose [Geobacillus stearothermophilus]

Swiss-Prot Hits      download full data without filtering help

Created with Snap36731101461832202562933303674034404775135505876236606971729sp|G4T4R7|AGASK_RUMGN1734sp|Q9ALJ4|AGAA_GEOSE3734sp|G1UB44|MELA_LACAC10734sp|P43467|AGAL1_PEDPE12733sp|P27756|AGAL_STRMU
Hit ID E-Value Query Start Query End Hit Start Hit End Description
G4T4R7 1.67e-235 1 729 1 715
Bifunctional alpha-galactosidase/sucrose kinase AgaSK OS=Ruminococcus gnavus OX=33038 GN=agaSK PE=1 SV=1
Q9ALJ4 5.36e-209 1 734 1 727
Alpha-galactosidase AgaA OS=Geobacillus stearothermophilus OX=1422 GN=agaA PE=1 SV=1
G1UB44 8.66e-192 3 734 6 731
Alpha-galactosidase Mel36A OS=Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) OX=272621 GN=melA PE=1 SV=1
P43467 4.61e-186 10 734 11 730
Alpha-galactosidase 1 OS=Pediococcus pentosaceus OX=1255 GN=agaR PE=3 SV=1
P27756 5.84e-156 12 733 10 719
Alpha-galactosidase OS=Streptococcus mutans serotype c (strain ATCC 700610 / UA159) OX=210007 GN=aga PE=3 SV=3

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
1.000068 0.000001 0.000000 0.000000 0.000000 0.000000

TMHMM  Annotations      help

There is no transmembrane helices in MGYG000004124_00251.