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CAZyme Information: MGYG000004199_00211

You are here: Home > Sequence: MGYG000004199_00211

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species
Lineage Bacteria; Firmicutes; Bacilli; RFN20; CAG-449; CAG-449;
CAZyme ID MGYG000004199_00211
CAZy Family GH36
CAZyme Description Alpha-galactosidase Mel36A
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
727 85531.64 5.7714
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000004199 1652569 MAG United Kingdom Europe
Gene Location Start: 22208;  End: 24391  Strand: +

Full Sequence      Download help

MIKYDEKNKI  FHLTNNSISY  YLYLNDAGYL  ETLYFGQYLS  EIFTSDGIRK  AYVDNNATEY60
FSFKDQKECT  YTDNFKSTYA  LLEMSSHGLN  DKRGAPVVLK  YNNGSTVTNF  KYVNHEIYKG120
IKPLSNLPHA  LNHDDCETLE  ILLKEKYHDI  FCKFYLTIYL  DKDIVVKNFE  IINKSDETVF180
IQKAFSMQLD  LNNVDYRFVH  FRGCWAKERD  YIINDVHDGI  QEVASNFGRS  SHDENPFVYL240
QKKDASNECG  EVIGFNLIYS  GNFKFRMFNS  YFHNLHITYG  INDEDFLWNL  GPNESFVTPQ300
AVISYSYQGV  DKMSQNFHRF  IKEDLITYEK  DKGYKPVLFN  SWEGCYFDFT  TESIISYIDD360
SKKINAELFV  LDDGWFGRRN  DDFDGLGDWW  INTKKIDLSK  VIKHCHDNNM  KFGIWFEPEM420
VNPKSDLFLN  NPSYILGESN  QDLTIHRHQF  HLDFSNKEVV  DNIYRQMVDI  LDKYQIDYIK480
WDYNRTVSES  FSQCYDASHQ  GEIYHRLVLG  YYDLISRLRE  RYPHIMFEGC  ASGGGRFDMG540
TLFYCPQIWC  SDESDPIQRI  FIQYNTSLGY  PLSTIGSHAN  ANPITSYKTK  ASIALFGSYG600
YEMNPNKLTK  TEIDELVEIA  SIYKKYHQEV  ICNGTLYHIL  SPVTTNFICM  MSVSQKKDKA660
LILLMNKLKE  TDQYRFIKLK  GLDEKKKYIN  NFDNRIYTGE  YLMKVGLNFT  FDWFDEFKCR720
LIEINEI727

Enzyme Prediction      help

EC 3.2.1.22

CAZyme Signature Domains help

Created with Snap367210914518121825429032736339943647250854558161765469010708GH36
Family Start End Evalue family coverage
GH36 10 708 5.8e-206 0.997093023255814

CDD Domains      download full data without filtering help

Created with Snap3672109145181218254290327363399436472508545581617654690295634Melibiase8707GalA334625GH3628291Glyco_hydro_36N649724Glyco_hydro_36C
Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
pfam02065 Melibiase 3.40e-136 295 634 1 347
Melibiase. Glycoside hydrolase families GH27, GH31 and GH36 form the glycoside hydrolase clan GH-D. Glycoside hydrolase family 36 can be split into 11 families, GH36A to GH36K. This family includes enzymes from GH36A-B and GH36D-K and from GH27.
COG3345 GalA 2.30e-133 8 707 1 665
Alpha-galactosidase [Carbohydrate transport and metabolism].
cd14791 GH36 1.06e-114 334 625 1 299
glycosyl hydrolase family 36 (GH36). GH36 enzymes occur in prokaryotes, eukaryotes, and archaea with a wide range of hydrolytic activities, including alpha-galactosidase, alpha-N-acetylgalactosaminidase, stachyose synthase, and raffinose synthase. All GH36 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH36 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.
pfam16875 Glyco_hydro_36N 3.98e-63 28 291 1 256
Glycosyl hydrolase family 36 N-terminal domain. This domain is found at the N-terminus of many family 36 glycoside hydrolases. It has a beta-supersandwich fold.
pfam16874 Glyco_hydro_36C 1.62e-14 649 724 3 78
Glycosyl hydrolase family 36 C-terminal domain. This domain is found at the C-terminus of many family 36 glycoside hydrolases. It has a beta-sandwich structure with a Greek key motif.

CAZyme Hits      help

Created with Snap36721091451812182542903273633994364725085455816176546902707AVQ19332.1|GH362707AKO93684.1|GH362707ATP38112.1|GH362707QLL71721.1|GH362707ARU19209.1|GH36
Hit ID E-Value Query Start Query End Hit Start Hit End
AVQ19332.1 5.74e-170 2 707 3 717
AKO93684.1 1.31e-169 2 707 3 710
ATP38112.1 8.54e-169 2 707 3 714
QLL71721.1 8.54e-169 2 707 3 714
ARU19209.1 8.54e-169 2 707 3 714

PDB Hits      download full data without filtering help

Created with Snap367210914518121825429032736339943647250854558161765469017062XN0_A17062XN2_A27074FNQ_A27074FNR_A27074FNP_A
Hit ID E-Value Query Start Query End Hit Start Hit End Description
2XN0_A 7.39e-168 1 706 5 711
Structureof alpha-galactosidase from Lactobacillus acidophilus NCFM, PtCl4 derivative [Lactobacillus acidophilus NCFM],2XN0_B Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM, PtCl4 derivative [Lactobacillus acidophilus NCFM],2XN1_A Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM with TRIS [Lactobacillus acidophilus NCFM],2XN1_B Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM with TRIS [Lactobacillus acidophilus NCFM],2XN1_C Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM with TRIS [Lactobacillus acidophilus NCFM],2XN1_D Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM with TRIS [Lactobacillus acidophilus NCFM]
2XN2_A 2.08e-167 1 706 5 711
Structureof alpha-galactosidase from Lactobacillus acidophilus NCFM with galactose [Lactobacillus acidophilus NCFM]
4FNQ_A 1.43e-147 2 707 3 708
Crystalstructure of GH36 alpha-galactosidase AgaB from Geobacillus stearothermophilus [Geobacillus stearothermophilus]
4FNR_A 3.46e-145 2 707 3 708
Crystalstructure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_B Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_C Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_D Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus]
4FNP_A 7.57e-144 2 707 3 708
Crystalstructure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNP_B Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNP_C Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNP_D Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNS_A Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus in complex with 1-deoxygalactonojirimycin [Geobacillus stearothermophilus],4FNS_B Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus in complex with 1-deoxygalactonojirimycin [Geobacillus stearothermophilus],4FNS_C Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus in complex with 1-deoxygalactonojirimycin [Geobacillus stearothermophilus],4FNS_D Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus in complex with 1-deoxygalactonojirimycin [Geobacillus stearothermophilus]

Swiss-Prot Hits      download full data without filtering help

Created with Snap36721091451812182542903273633994364725085455816176546901706sp|G1UB44|MELA_LACAC2707sp|Q9ALJ4|AGAA_GEOSE1706sp|P43467|AGAL1_PEDPE2707sp|G4T4R7|AGASK_RUMGN87707sp|P27756|AGAL_STRMU
Hit ID E-Value Query Start Query End Hit Start Hit End Description
G1UB44 4.04e-167 1 706 5 711
Alpha-galactosidase Mel36A OS=Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) OX=272621 GN=melA PE=1 SV=1
Q9ALJ4 1.89e-144 2 707 3 708
Alpha-galactosidase AgaA OS=Geobacillus stearothermophilus OX=1422 GN=agaA PE=1 SV=1
P43467 2.99e-144 1 706 3 710
Alpha-galactosidase 1 OS=Pediococcus pentosaceus OX=1255 GN=agaR PE=3 SV=1
G4T4R7 7.64e-131 2 707 3 701
Bifunctional alpha-galactosidase/sucrose kinase AgaSK OS=Ruminococcus gnavus OX=33038 GN=agaSK PE=1 SV=1
P27756 7.24e-118 87 707 76 701
Alpha-galactosidase OS=Streptococcus mutans serotype c (strain ATCC 700610 / UA159) OX=210007 GN=aga PE=3 SV=3

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
1.000046 0.000000 0.000000 0.000000 0.000000 0.000000

TMHMM  Annotations      help

There is no transmembrane helices in MGYG000004199_00211.