CAZyme Information: MGYG000000440_00450

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; CAG-272; UMGS1865
• CAZyme ID: MGYG000000440_00450
• CAZy Family: GH38
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1009		115299	5.6948

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000440	2422037	MAG	Sweden	Europe

• Gene Location: Start: 108207; End: 111236 Strand: +

Full Sequence      

MQEFIRELKKYSEILDANRAMSLGRITDVRWSPCGYKQAGELPNDSALTPFDTEKDTWGN
GYDAHAWFRFAVDVPQDFENIFLSVATDRVGTWDPDNPQLLVWVDGNMEQGMDINHTRLY
FRSAGRHEITVYAYTGIKALSSCFRPELFRLNRDVEKLWYDIKVPFDSLSYLEPYSGEYR
TILSNLNRAVLLLDLCEVPSEAFHTSVKVASEWMDREFYGKLCHAPDENAPVTVGIGHTH
IDCAWLWTLKQTREKVQRSFTNSLMLMERYPEYRFMSSQALLYQNLKEEAPEIYEKVKKA
IRDGKWEVEGAMWVEADCNLTGGESLVRQVLYGKRFFRDEFGVESHVLWLPDVFGYSAAL
PQILRKSGVDWFVTSKISWNDTNMMPYDTFLWRGIDGTGINSYFLTAQDAKRGKDPERYT
TYVAHITPAQVAGTWKRYQQKELNNETLITFGYGDGGGGSTEADLEIGRRLSKGIPGTPA
FKVDFAGNLLSRLEKKIENNLRLPVWQGELYLEFHRGTYTTQSRNKKNNRRSESLYLATE
WLCSLNRVLFDTAFPKADLHKGWEMILTNQFHDIIPGSSIRQVYEQCEKDYAAIRSLAEP
HKTQAEAKLAANLSKANGYVVINPNPTEGNGIVRLNGKSVAVNNIPPKGYACVKAFDETN
TAKISRTECENRRYRLTFDGTMRITSFYDKENRREVLKKGGFGNELRIFNDYVGYNDAWD
LESYNLQKYISLTDVQSVEEVQDGVRAGLQIVRKHGKSTFEQTIWLSDATDRVDFDLKVD
WHERHQCVKVAFDMAINAARATYEIQYGTIERPTHKNTSWDSAKFEVCGHRFADLSEGNY
GVTLFNDCKYGYDIHDSLMTLTLLRAPTYPDPEADRGEMTCTYSVVPHRGAMDAPKTYAL
AYDLNNPMTVLPATGEKDSIPTEFCTVASDAPNILCETVKQAEDGEDTVCRFFECSNTAT
TATLRFGHPVKKVMLCDMSENDLQELTVRDNAVTLDFRAFEIQTLKVKK

Enzyme Prediction     

EC	3.2.1.24

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH38	236	498	9.6e-80	0.929368029739777

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0383	AMS1	3.83e-161	235	1009	202	943	Alpha-mannosidase [Carbohydrate transport and metabolism].
cd10789	GH38N_AMII_ER_cytosolic	2.37e-117	236	496	5	252	N-terminal catalytic domain of endoplasmic reticulum(ER)/cytosolic class II alpha-mannosidases; glycoside hydrolase family 38 (GH38). The subfamily is represented by Saccharomyces cerevisiae vacuolar alpha-mannosidase Ams1, rat ER/cytosolic alpha-mannosidase Man2C1, and similar proteins. Members in this family share high sequence similarity. None of them have any classical signal sequence or membrane spanning domains, which are typical of sorting or targeting signals. Ams1 functions as a second resident vacuolar hydrolase in S. cerevisiae. It aids in recycling macromolecular components of the cell through hydrolysis of terminal, non-reducing alpha-d-mannose residues. Ams1 utilizes both the cytoplasm to vacuole targeting (Cvt, nutrient-rich conditions) and autophagic (starvation conditions) pathways for biosynthetic delivery to the vacuole. Man2C1is involved in oligosaccharide catabolism in both the ER and cytosol. It can catalyze the cobalt-dependent cleavage of alpha 1,2-, alpha 1,3-, and alpha 1,6-linked mannose residues. Members in this family are retaining glycosyl hydrolases of family GH38 that employs a two-step mechanism involving the formation of a covalent glycosyl-enzyme complex. Two carboxylic acids positioned within the active site act in concert: one as a catalytic nucleophile and the other as a general acid/base catalyst.
pfam01074	Glyco_hydro_38	3.12e-87	235	451	4	214	Glycosyl hydrolases family 38 N-terminal domain. Glycosyl hydrolases are key enzymes of carbohydrate metabolism.
pfam07748	Glyco_hydro_38C	2.99e-72	670	873	2	204	Glycosyl hydrolases family 38 C-terminal domain. Glycosyl hydrolases are key enzymes of carbohydrate metabolism.
cd10813	GH38N_AMII_Man2C1	1.48e-69	236	496	5	252	N-terminal catalytic domain of mammalian cytosolic alpha-mannosidase Man2C1 and similar proteins; glycoside hydrolase family 38 (GH38). The subfamily corresponds to cytosolic alpha-mannosidase Man2C1 (also known as ER-mannosidase II or neutral/cytosolic mannosidase), mainly found in various vertebrates, and similar proteins. Man2C1 plays an essential role in the catabolism of cytosolic free oligomannosides derived from dolichol intermediates and the degradation of newly synthesized glycoproteins in ER or cytosol. It can catalyze the cleavage of alpha 1,2-, alpha 1,3-, and alpha 1,6-linked mannose residues. Man2C1 is a cobalt-dependent enzyme belonging to alpha-mannosidase class II. It has a neutral pH optimum and is strongly inhitibed by furanose analogs swainsonine (SW) and 1,4-dideoxy-1,4-imino-D-mannitol (DIM), moderately by deoxymannojirimycin (DMM), but not by kifunensine (KIF). DMM and KIF, both pyranose analogs, are normally known to inhibit class I alpha-mannosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QGT50950.1	2.94e-299	1	1005	1	1004
ADH61407.1	2.97e-297	1	1007	8	1042
ADD02959.1	6.25e-297	1	1007	8	1042
ABY94276.1	3.36e-296	30	1007	30	1042
ADV79229.1	3.36e-296	30	1007	30	1042

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6LZ1_A	5.26e-137	236	1007	286	1075	Structureof S.pombe alpha-mannosidase Ams1 [Schizosaccharomyces pombe 972h-],6LZ1_B Structure of S.pombe alpha-mannosidase Ams1 [Schizosaccharomyces pombe 972h-],6LZ1_C Structure of S.pombe alpha-mannosidase Ams1 [Schizosaccharomyces pombe 972h-],6LZ1_D Structure of S.pombe alpha-mannosidase Ams1 [Schizosaccharomyces pombe 972h-]
7DD9_A	9.92e-134	236	1007	286	1075	ChainA, Alpha-mannosidase,ZZ-type zinc finger-containing protein P35G2.11c,Maltose/maltodextrin-binding periplasmic protein [synthetic construct],7DD9_C Chain C, Alpha-mannosidase,ZZ-type zinc finger-containing protein P35G2.11c,Maltose/maltodextrin-binding periplasmic protein [synthetic construct],7DD9_E Chain E, Alpha-mannosidase,ZZ-type zinc finger-containing protein P35G2.11c,Maltose/maltodextrin-binding periplasmic protein [synthetic construct],7DD9_G Chain G, Alpha-mannosidase,ZZ-type zinc finger-containing protein P35G2.11c,Maltose/maltodextrin-binding periplasmic protein [synthetic construct]
5JM0_A	2.12e-119	205	1007	269	1094	Structureof the S. cerevisiae alpha-mannosidase 1 [Saccharomyces cerevisiae S288C]
1HWW_A	2.38e-06	236	404	56	230	GOLGIALPHA-MANNOSIDASE II IN COMPLEX WITH SWAINSONINE [Drosophila melanogaster],1HXK_A Golgi Alpha-Mannosidase Ii In Complex With Deoxymannojirimicin [Drosophila melanogaster]
1PS3_A	2.40e-06	236	404	86	260	Golgialpha-mannosidase II in complex with kifunensine [Drosophila melanogaster]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9NTJ4	7.66e-151	67	1005	81	1031	Alpha-mannosidase 2C1 OS=Homo sapiens OX=9606 GN=MAN2C1 PE=1 SV=1
Q91W89	1.34e-145	67	1005	80	1030	Alpha-mannosidase 2C1 OS=Mus musculus OX=10090 GN=Man2c1 PE=1 SV=1
Q54K67	8.42e-144	67	1006	83	1083	Alpha-mannosidase G OS=Dictyostelium discoideum OX=44689 GN=manG PE=1 SV=1
Q9UT61	8.59e-137	236	1007	286	1075	Alpha-mannosidase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ams1 PE=1 SV=1
P21139	7.77e-135	67	1005	80	1031	Alpha-mannosidase 2C1 OS=Rattus norvegicus OX=10116 GN=Man2c1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000040	0.000003	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000440_00450.