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CAZyme Information: MGYG000004667_00112

You are here: Home > Sequence: MGYG000004667_00112

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species Clostridium beijerinckii
Lineage Bacteria; Firmicutes_A; Clostridia; Clostridiales; Clostridiaceae; Clostridium; Clostridium beijerinckii
CAZyme ID MGYG000004667_00112
CAZy Family GH4
CAZyme Description Phospho-alpha-glucosidase PagL
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
440 MGYG000004667_11|CGC2 50581.87 5.2997
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000004667 6180974 Isolate United States North America
Gene Location Start: 79364;  End: 80686  Strand: -

Full Sequence      Download help

MKKFNVSLVG  GGSTWTPGLL  TSLCKLKDRF  PINRLVLFDI  DKERQETIAN  YAKVLFGEKY60
PELDFKYTTN  VEEAYIDVDF  VIMQMRTGGY  KMREQDEKIP  LSLGLIGQET  CGAGGFAYGL120
RSIRDMIKTI  KDIRKYSKDA  WVLNYTNPAA  IVAEALRREF  PDDKRVLNIC  DQPVNLLRSY180
GRVLGKDSKN  FEPVYFGLNH  FGWFTNLYDE  NGNDLVPELR  KIIKEKGFIP  VDAEQRDKSW240
LDTYAMVQDM  VNDFPEYLPN  TYLQYYYYPD  YKVSHLNPDY  TRANEVMDGR  EKRVFEECRR300
VFEAGTVEGS  VMVHNDAHSE  FMIEVAEAIA  FNLKKIYIVI  VKNNGIISNL  DDDVMVEVAS360
VMTANGPRPL  AVGKIPTFYK  GLIESQSAYE  KLTVDAYFEG  SYEKALQALT  LNRTIVSAKK420
AREVLDKLIE  ANKEYWPEFK  440

Enzyme Prediction      help

EC 3.2.1.122

CAZyme Signature Domains help

Created with Snap224466881101321541761982202422642863083303523743964186182GH4
Family Start End Evalue family coverage
GH4 6 182 5.1e-64 0.9776536312849162

CDD Domains      download full data without filtering help

Created with Snap224466881101321541761982202422642863083303523743964184440GH4_GlvA_pagL_like1440CelF4431GH4_glycoside_hydrolases1435GH4_P_beta_glucosidase195415Glyco_hydro_4C
Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
cd05298 GH4_GlvA_pagL_like 0.0 4 440 1 437
Glycoside Hydrolases Family 4; GlvA- and pagL-like glycosidases. Bacillus subtilis GlvA and Clostridium acetobutylicum pagL are 6-phospho-alpha-glucosidase, catalyzing the hydrolysis of alpha-glucopyranoside bonds to release glucose from oligosaccharides. The substrate specificities of other members of this subgroup are unknown. Some bacteria simultaneously translocate and phosphorylate disaccharides via the phosphoenolpyruvate-dependent phosphotransferase system (PEP_PTS). After translocation, these phospho-disaccharides may be hydrolyzed by the GH4 glycoside hydrolases, which include 6-phospho-beta-glucosidases, 6-phospho-alpha-glucosidases, alpha-glucosidases/alpha-glucuronidases (only from Thermotoga), and alpha-galactosidases. Members of this subfamily are part of the NAD(P)-binding Rossmann fold superfamily, which includes a wide variety of protein families including the NAD(P)-binding domains of alcohol dehydrogenases, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate dehydrogenases, formate/glycerate dehydrogenases, siroheme synthases, 6-phosphogluconate dehydrogenases, aminoacid dehydrogenases, repressor rex, and NAD-binding potassium channel domains, among others.
COG1486 CelF 2.08e-151 1 440 1 440
Alpha-galactosidase/6-phospho-beta-glucosidase, family 4 of glycosyl hydrolase [Carbohydrate transport and metabolism].
cd05197 GH4_glycoside_hydrolases 2.94e-121 4 431 1 425
Glycoside Hydrases Family 4. Glycoside hydrolases cleave glycosidic bonds to release smaller sugars from oligo- or polysaccharides. Some bacteria simultaneously translocate and phosphorylate disaccharides via the phosphoenolpyruvate-dependent phosphotransferase system (PEP-PTS). After translocation, these phospho-disaccharides may be hydrolyzed by GH4 glycoside hydrolases. Other organisms (such as archaea and Thermotoga maritima) lack the PEP-PTS system, but have several enzymes normally associated with the PEP-PTS operon. GH4 family members include 6-phospho-beta-glucosidases, 6-phospho-alpha-glucosidases, alpha-glucosidases/alpha-glucuronidases (only from Thermotoga), and alpha-galactosidases. They require two cofactors, NAD+ and a divalent metal (Mn2+, Ni2+, Mg2+), for activity. Some also require reducing conditions. GH4 glycoside hydrolases are part of the NAD(P)-binding Rossmann fold superfamily, which includes a wide variety of protein families including the NAD(P)-binding domains of alcohol dehydrogenases, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate dehydrogenases, formate/glycerate dehydrogenases, siroheme synthases, 6-phosphogluconate dehydrogenases, aminoacid dehydrogenases, repressor rex, and NAD-binding potassium channel domains, among others.
cd05296 GH4_P_beta_glucosidase 2.23e-111 1 435 1 418
Glycoside Hydrolases Family 4; Phospho-beta-glucosidase. Some bacteria simultaneously translocate and phosphorylate disaccharides via the phosphoenolpyruvate-dependent phosphotransferase system (PEP-PTS). After translocation, these phospho-disaccharides may be hydrolyzed by the GH4 glycoside hydrolases such as the phospho-beta-glucosidases. Other organisms (such as archaea and Thermotoga maritima ) lack the PEP-PTS system, but have several enzymes normally associated with the PEP-PTS operon. The 6-phospho-beta-glucosidase from Thermotoga maritima hydrolylzes cellobiose 6-phosphate (6P) into glucose-6P and glucose, in an NAD+ and Mn2+ dependent fashion. The Escherichia coli 6-phospho-beta-glucosidase (also called celF) hydrolyzes a variety of phospho-beta-glucosides including cellobiose-6P, salicin-6P, arbutin-6P, and gentobiose-6P. Phospho-beta-glucosidases are part of the NAD(P)-binding Rossmann fold superfamily, which includes a wide variety of protein families including the NAD(P)-binding domains of alcohol dehydrogenases, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate dehydrogenases, formate/glycerate dehydrogenases, siroheme synthases, 6-phosphogluconate dehydrogenases, aminoacid dehydrogenases, repressor rex, and NAD-binding potassium channel domains, among others.
pfam11975 Glyco_hydro_4C 2.39e-52 195 415 1 168
Family 4 glycosyl hydrolase C-terminal domain.

CAZyme Hits      help

Created with Snap224466881101321541761982202422642863083303523743964181440AIU02396.1|GH41440QUN35151.1|GH41440ABR37090.1|GH41440QUF74075.1|GH41440QAT40937.1|GH4
Hit ID E-Value Query Start Query End Hit Start Hit End
AIU02396.1 2.07e-316 1 440 1 440
QUN35151.1 2.07e-316 1 440 1 440
ABR37090.1 2.07e-316 1 440 1 440
QUF74075.1 2.07e-316 1 440 1 440
QAT40937.1 3.83e-312 1 440 1 440

PDB Hits      download full data without filtering help

Created with Snap2244668811013215417619822024226428630833035237439641824406VC6_A24386DUX_A24381U8X_X24395C3M_A24391S6Y_A
Hit ID E-Value Query Start Query End Hit Start Hit End Description
6VC6_A 1.16e-196 2 440 2 441
2.1Angstrom Resolution Crystal Structure of 6-phospho-alpha-glucosidase from Gut Microorganisms in Complex with NAD and Mn2+ [Merdibacter massiliensis],6VC6_B 2.1 Angstrom Resolution Crystal Structure of 6-phospho-alpha-glucosidase from Gut Microorganisms in Complex with NAD and Mn2+ [Merdibacter massiliensis],6VC6_C 2.1 Angstrom Resolution Crystal Structure of 6-phospho-alpha-glucosidase from Gut Microorganisms in Complex with NAD and Mn2+ [Merdibacter massiliensis],6VC6_D 2.1 Angstrom Resolution Crystal Structure of 6-phospho-alpha-glucosidase from Gut Microorganisms in Complex with NAD and Mn2+ [Merdibacter massiliensis]
6DUX_A 1.40e-146 2 438 5 441
ChainA, 6-phospho-alpha-glucosidase [Klebsiella pneumoniae],6DUX_B Chain B, 6-phospho-alpha-glucosidase [Klebsiella pneumoniae],6DVV_A Chain A, 6-phospho-alpha-glucosidase [Klebsiella pneumoniae],6DVV_B Chain B, 6-phospho-alpha-glucosidase [Klebsiella pneumoniae]
1U8X_X 3.60e-141 2 438 27 463
CrystalStructure Of Glva From Bacillus Subtilis, A Metal-requiring, Nad-dependent 6-phospho-alpha-glucosidase [Bacillus subtilis]
5C3M_A 2.03e-60 2 439 3 437
Crystalstructure of Gan4C, a GH4 6-phospho-glucosidase from Geobacillus stearothermophilus [Geobacillus stearothermophilus],5C3M_B Crystal structure of Gan4C, a GH4 6-phospho-glucosidase from Geobacillus stearothermophilus [Geobacillus stearothermophilus],5C3M_C Crystal structure of Gan4C, a GH4 6-phospho-glucosidase from Geobacillus stearothermophilus [Geobacillus stearothermophilus],5C3M_D Crystal structure of Gan4C, a GH4 6-phospho-glucosidase from Geobacillus stearothermophilus [Geobacillus stearothermophilus]
1S6Y_A 1.24e-58 2 439 6 440
2.3Acrystal structure of phospho-beta-glucosidase [Geobacillus stearothermophilus]

Swiss-Prot Hits      download full data without filtering help

Created with Snap224466881101321541761982202422642863083303523743964181438sp|Q97DP6|PAGL_CLOAB1440sp|O06901|MALH_FUSMR2440sp|Q034G9|PAGL2_LACP31438sp|Q97LM4|MALH_CLOAB1440sp|C7NB67|PAGL_LEPBD
Hit ID E-Value Query Start Query End Hit Start Hit End Description
Q97DP6 2.05e-166 1 438 1 443
Phospho-alpha-glucosidase PagL OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=pagL PE=1 SV=1
O06901 1.57e-157 1 440 1 441
Maltose-6'-phosphate glucosidase OS=Fusobacterium mortiferum OX=850 GN=malH PE=1 SV=1
Q034G9 1.77e-156 2 440 5 444
6-phospho-alpha-glucosidase 2 OS=Lacticaseibacillus paracasei (strain ATCC 334 / BCRC 17002 / CCUG 31169 / CIP 107868 / KCTC 3260 / NRRL B-441) OX=321967 GN=LSEI_2684 PE=3 SV=1
Q97LM4 1.03e-155 1 438 1 439
Maltose-6'-phosphate glucosidase MalH OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=malH PE=1 SV=1
C7NB67 2.30e-154 1 440 1 440
6-phospho-alpha-glucosidase OS=Leptotrichia buccalis (strain ATCC 14201 / DSM 1135 / JCM 12969 / NCTC 10249 / C-1013-b) OX=523794 GN=pagL PE=1 SV=1

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
1.000039 0.000000 0.000000 0.000000 0.000000 0.000000

TMHMM  Annotations      help

There is no transmembrane helices in MGYG000004667_00112.