CAZyme Information: MGYG000001560_00072

Basic Information

• Species: Massilioclostridium coli
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Massilioclostridium; Massilioclostridium coli
• CAZyme ID: MGYG000001560_00072
• CAZy Family: CBM67
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1876	MGYG000001560_1|CGC2	208343.4	4.1394

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001560	2985330	Isolate	not provided	not provided

• Gene Location: Start: 96897; End: 102527 Strand: +

Full Sequence      

MKKFYRSIAAILAVCMLFTMAFTVSATDQTLYTMEILDLTVNNRTNPQGLDDDTPSFGWR
MDSNLVGQKQTAYHVIVTEKFSGETMWDSGKVEGNVSQYIEYAGKELSPESDYNWNVTVY
DSNGKAYLSDTATFTTGLMADRLIPEEQPSIQDLTSYTIDTDFVIHSGCMGLLFGVQDSN
NFYMWQINAGDLSINPHQWSNGGINTNFPHPSIKDALGVDSILETPLHLTMKMEEGTVTS
WINDVQIGEPLSIGQVKLGRIGFRQAVNESVDIDQITVKDQDGQVVFEEDFSDQTSNPFD
GGTVENGQLKMNSTNLVFQKDTELENTTFTIDFDGQVVNTALGPLIGGQDNSNFYMWQIN
AAERVFKPHQWSGGNITTLKQVSLDPVFGEGQPIEGVPFHMTIQANNGEVKTYLNKIGEQ
PQLIDTIQLSPFSVGKFGFRQMKDHVSNEVGLIDNLKIVGENGSILLEDDFSSPYNTTFP
SGSITEDGWFCAENGLFFQNETEEVLGWSGAQWIGSPEISVDSGKIPVYNLYYDLQLVEG
STKAAALVCGNDYRLMDKTKNNYRVEGESYLSFELDASNVSAGGSPVLNLYRKGFCSDDY
KNPDATTLMRSFSLDNIGVTAENLYTTPIRFSLTCYGGGFRIYVNDQNYGEVVINPTGGT
QDQPCYTYLNSIGFLTEPGQQAEFQNYEIRTWDTAHAVMFDKTTGATYSIFEGQDGVSVQ
DHFIMVGDQAKQVKILAEPDYKSNQLLRKEFNADQPIQSARLYITSRGIYEPYVNGERIG
EDWFNPGFTQYNKTITYSTYDITDQLQQGTNAIGVRLASGWWHDEMTFDLSKYNYWGNKP
SLLAKVVVTYADGTKDTIVTDESWSYNNTDSPIIYAGFFNGEVYDARKEATIEGWNQPGY
TGENWYNASVIVPLPENANPHIIARVDEPVRHVETLSAKFDKEPDPNIYIYDMGTNMVGV
PEITFPEMPAGTEITIRYSEIYYPELDPSNPYYYGEMSGKLLTENLRAALCTDTYIAKGT
KGGETYRPSFTFHGYRYIEISGLEEPLPEENIKGIVLSSIEGTTSTYESSNELTNQLHKN
IQRSLLGNHVSIPTDCPQRDERMGWTGDAQVFSRTATYYGDMNLLYEGWEGTIRDAQTAS
GTIPQTAPNLGWGASVEVAWPAAIVIPTWEIYKQYGNTRVIESNLDAMKAFLDSIGNDKF
APDSYLTKGTALTEHLALVGTDSPLCSNVLYAYMLGLFSKMAAAIGEDELAQHYGDLSVK
VKEEWNQVFINAATGQTQNSSGGIQDTQASYALAIEYNVISDENRDRAAELLDQACERGY
NNVPYTITTGFIGTAPLLPALTDVGKVDTAYKMFEQTEFASWLYPVTQGATSIWERWNGY
TIENGFSGLNYMNSFNHYAAGAVGAWMMNDHVGITSDEEHPGFQQFILQPTLGGDFTYVN
GGYDSIYGHITSNWKVNQGKLTAYEAEVPANTTATLYLPMDQNTVIGDLPEGVSFTGWDT
RYGNSVAVFQLVAGGYQFSVENNGLTVALNDGYVTEGTPEPPVGDANKTILDKVITEANR
LKETEEYTNAIPAVKESFDKVLADAQAVYDNPSATQKEVDTAWMNLMEEIHKLGFQKGDK
TALQELYYQVKDIDLSQYRDGAAKENFKTALVNAETVLADENALQNEIDKAYNDLKSAYE
ALEKLADKSQLKALLDECAGYQKEEYTPVTWEVFEGIQKKAQEVYDNANATQEEINAAVD
ELLSGMLQLRFKADKSLLQNLIAELEEMDFSQYTEESVAAFQIVLAEAKTILSDDNISIE
DQQIVDQQVSNLKSAYKGLIGKQTDSTEFVDITPSNENSQETKPIEAANTGDVSPIAGLI
LLGLAGITVAAFQKRK

Enzyme Prediction     

EC	3.2.1.40	3.1.1.73

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH78	944	1478	2.3e-142	0.9920634920634921
CBM67	740	914	5.5e-34	0.9034090909090909

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam17389	Bac_rhamnosid6H	8.44e-97	1064	1407	3	336	Bacterial alpha-L-rhamnosidase 6 hairpin glycosidase domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam08531	Bac_rhamnosid_N	6.72e-60	756	934	2	172	Alpha-L-rhamnosidase N-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. This domain is probably involved in substrate recognition.
pfam05592	Bac_rhamnosid	5.22e-28	942	1058	1	102	Bacterial alpha-L-rhamnosidase concanavalin-like domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam17390	Bac_rhamnosid_C	6.80e-17	1421	1479	7	64	Bacterial alpha-L-rhamnosidase C-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam06008	Laminin_I	6.58e-05	1580	1749	65	252	Laminin Domain I. coiled-coil structure. It has been suggested that the domains I and II from laminin A, B1 and B2 may come together to form a triple helical coiled-coil structure.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QGH33563.1	1.48e-306	486	1569	142	1230
AIF65800.1	6.47e-233	485	1518	116	1159
QGH37059.1	7.28e-230	485	1518	125	1180
QOG04730.1	4.06e-229	499	1517	152	1192
AOZ98905.1	2.67e-228	503	1523	140	1182

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3W5M_A	6.34e-134	743	1521	302	1031	CrystalStructure of Streptomyces avermitilis alpha-L-rhamnosidase [Streptomyces avermitilis MA-4680 = NBRC 14893],3W5N_A Crystal Structure of Streptomyces avermitilis alpha-L-rhamnosidase complexed with L-rhamnose [Streptomyces avermitilis MA-4680 = NBRC 14893]
6I60_A	6.19e-109	746	1521	178	942	Structureof alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12],6I60_B Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12]
6GSZ_A	1.53e-105	748	1479	135	847	Crystalstructure of native alfa-L-rhamnosidase from Aspergillus terreus [Aspergillus terreus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q82PP4	3.51e-133	743	1520	302	1030	Alpha-L-rhamnosidase OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=SAVERM_828 PE=1 SV=1
T2KNB2	2.57e-119	727	1483	162	883	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22090 PE=1 SV=2
P9WF03	6.24e-104	736	1520	167	910	Alpha-L-rhamnosidase OS=Alteromonas sp. (strain LOR) OX=1537994 GN=LOR_34 PE=1 SV=1
T2KPL4	5.49e-59	748	1478	188	906	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22170 PE=2 SV=1
E8MGH9	4.77e-08	1687	1819	1667	1803	Beta-L-arabinobiosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA2 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000354	0.998771	0.000254	0.000207	0.000199	0.000182

TMHMM  Annotations     

start	end
7	26
1853	1872