CAZyme Information: MGYG000004379_00903

Basic Information

• Species: CAG-628 sp000438415
• Lineage: Bacteria; Firmicutes; Bacilli; RF39; UBA660; CAG-628; CAG-628 sp000438415
• CAZyme ID: MGYG000004379_00903
• CAZy Family: GT26
• CAZyme Description: UDP-N-acetyl-D-mannosaminuronic acid transferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
719	MGYG000004379_22|CGC1	84589.29	9.9939

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004379	1274831	MAG	Israel	Asia

• Gene Location: Start: 14705; End: 16864 Strand: +

Full Sequence      

MKNLNKQKLINIIMILYVLSILLDLHIFYNPVSTLIRIIFISLIFLIIFIKYSTKKEKKL
LITYFILLLIYIILHLVSTSNFKVETNYNLYEELLYFIKMSMNILIIYSIYKLSISKNKF
YKLIEISAFIISFSIIITNIFKIGYTSYNFQHPKYNIFEWFYKNTNFLEASTKGFFHLTN
QISGILLLYIGVLLLSIKNKQKIFNTITLTLSVISMFILGTRVSSYSVFIILGISLIGYI
LTTIKDSKIKLSIILTHIILLLMSILLYKYSPLQSRNAYYKELFKEREVNRSSITIEEAL
NLSDKEFKKLLLNYNIVPEFYNKYYPLEKDRDFYEEYISRNTTKINDTRYLEASIIKRVK
SLNNNNKDNIYGIGYNRIMNIFNIENDFIMQYYSVGYIGVIMLLGVYIVILIYLYLKTLF
NLEKYFTYENGMLLFITTYYLICSFYTGNILNAISTILPLSFVMGYHLSIMNKKEKEDFE
YFIGFKTSTKNIEEITDEIMSSEKQNIIYNINPLILMNFYKDIKSVDEFNKENYNIPDGN
GIVISSKLTSGNINKSIPGVELFEKICQFSVKKKYKIYLYGAKQSSVYNTKVGLETKYKG
IKIVGFKNGYISEKEAVNDIIKTKPDILFVALGSPKQEKFIMKNKNKFKNIKIIMPVGGT
FDVVSKNTLRAPLIYRNLKLEWLYRMIKEPKRFKQLGTLFKYMLTILFLNTYYNRKAKF

Enzyme Prediction     

No EC number prediction in MGYG000004379_00903.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT26	530	694	3.5e-49	0.9707602339181286

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam03808	Glyco_tran_WecB	9.05e-47	534	694	3	166	Glycosyl transferase WecB/TagA/CpsF family.
cd06533	Glyco_transf_WecG_TagA	9.78e-47	529	692	1	166	The glycosyltransferase WecG/TagA superfamily contains Escherichia coli WecG, Bacillus subtilis TagA and related proteins. E. coli WecG is believed to be a UDP-N-acetyl-D-mannosaminuronic acid transferase, and is involved in enterobacterial common antigen (eca) synthesis. B. subtilis TagA plays a key role in the Wall Teichoic Acid (WTA) biosynthetic pathway, catalyzing the transfer of N-acetylmannosamine to the C4 hydroxyl of a membrane-anchored N-acetylglucosaminyl diphospholipid to make ManNAc-beta-(1,4)-GlcNAc-pp-undecaprenyl. This is the first committed step in this pathway. Also included in this group is Xanthomonas campestris pv. campestris GumM, a glycosyltransferase participating in the biosynthesis of the exopolysaccharide xanthan.
COG1922	WecG	3.37e-45	492	718	26	252	UDP-N-acetyl-D-mannosaminuronic acid transferase, WecB/TagA/CpsF family [Cell wall/membrane/envelope biogenesis].
TIGR00696	wecG_tagA_cpsF	3.12e-38	530	702	4	177	bacterial polymer biosynthesis proteins, WecB/TagA/CpsF family. The WecG member of this superfamily, believed to be UDP-N-acetyl-D-mannosaminuronic acid transferase, plays a role in enterobacterial common antigen (eca) synthesis in Escherichia coli. Another family member, the Bacillus subtilis TagA protein, is involved in the biosynthesis of the cell wall polymer poly(glycerol phosphate). The third family member, CpsF, CMP-N-acetylneuraminic acid synthetase has a role in the capsular polysaccharide biosynthesis pathway. [Cell envelope, Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides]
PRK03692	PRK03692	6.82e-24	534	716	64	242	putative UDP-N-acetyl-D-mannosaminuronic acid transferase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNU36053.1	3.48e-49	480	707	4	234
AGB18231.1	1.27e-48	486	707	14	237
QXJ38755.1	4.65e-48	480	707	4	234
QNU39510.1	4.65e-48	480	707	4	234
ADL68106.1	4.93e-48	486	708	14	238

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7MPK_A	2.49e-39	494	707	21	236	ChainA, N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase [Thermoanaerobacter italicus Ab9],7MPK_B Chain B, N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase [Thermoanaerobacter italicus Ab9],7MPK_C Chain C, N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase [Thermoanaerobacter italicus Ab9],7N41_A Chain A, N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase [Thermoanaerobacter italicus Ab9],7N41_B Chain B, N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase [Thermoanaerobacter italicus Ab9],7N41_C Chain C, N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase [Thermoanaerobacter italicus Ab9]
5WB4_A	1.18e-26	494	665	21	194	Crystalstructure of the TarA wall teichoic acid glycosyltransferase [Thermoanaerobacter italicus Ab9],5WB4_B Crystal structure of the TarA wall teichoic acid glycosyltransferase [Thermoanaerobacter italicus Ab9],5WB4_C Crystal structure of the TarA wall teichoic acid glycosyltransferase [Thermoanaerobacter italicus Ab9],5WB4_D Crystal structure of the TarA wall teichoic acid glycosyltransferase [Thermoanaerobacter italicus Ab9],5WB4_E Crystal structure of the TarA wall teichoic acid glycosyltransferase [Thermoanaerobacter italicus Ab9],5WB4_F Crystal structure of the TarA wall teichoic acid glycosyltransferase [Thermoanaerobacter italicus Ab9],5WB4_G Crystal structure of the TarA wall teichoic acid glycosyltransferase [Thermoanaerobacter italicus Ab9],5WB4_H Crystal structure of the TarA wall teichoic acid glycosyltransferase [Thermoanaerobacter italicus Ab9]
5WFG_A	2.18e-26	494	665	21	194	Crystalstructure of the TarA wall teichoic acid glycosyltransferase bound to UDP [Thermoanaerobacter italicus Ab9],5WFG_B Crystal structure of the TarA wall teichoic acid glycosyltransferase bound to UDP [Thermoanaerobacter italicus Ab9],5WFG_C Crystal structure of the TarA wall teichoic acid glycosyltransferase bound to UDP [Thermoanaerobacter italicus Ab9],5WFG_D Crystal structure of the TarA wall teichoic acid glycosyltransferase bound to UDP [Thermoanaerobacter italicus Ab9],5WFG_E Crystal structure of the TarA wall teichoic acid glycosyltransferase bound to UDP [Thermoanaerobacter italicus Ab9],5WFG_F Crystal structure of the TarA wall teichoic acid glycosyltransferase bound to UDP [Thermoanaerobacter italicus Ab9]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P27620	8.18e-37	507	707	35	235	N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase OS=Bacillus subtilis (strain 168) OX=224308 GN=tagA PE=1 SV=1
Q8RKI7	4.03e-29	489	707	17	235	N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase OS=Bacillus spizizenii (strain ATCC 23059 / NRRL B-14472 / W23) OX=655816 GN=tarA PE=3 SV=1
Q8NXS7	2.67e-23	487	699	23	236	N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase OS=Staphylococcus aureus (strain MW2) OX=196620 GN=tarA PE=3 SV=1
Q6GBJ4	4.91e-23	487	699	23	236	N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase OS=Staphylococcus aureus (strain MSSA476) OX=282459 GN=tarA PE=3 SV=1
Q9LC45	4.91e-23	487	699	23	236	N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase OS=Staphylococcus aureus OX=1280 GN=tarA PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000023	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
9	29
34	53
60	79
94	116
123	145
175	197
218	240
253	270
394	416
431	453
696	713