CAZyme Information: MGYG000001115_00501

Basic Information

• Species: Agathobaculum sp900544475
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Butyricicoccaceae; Agathobaculum; Agathobaculum sp900544475
• CAZyme ID: MGYG000001115_00501
• CAZy Family: GT35
• CAZyme Description: Glycogen phosphorylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
815		92083.12	5.7566

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001115	2249105	MAG	China	Asia

• Gene Location: Start: 344; End: 2791 Strand: -

Full Sequence      

MAKTQYTKAALKEAITGKLQRHFACEVKDAKKDQIYEACALVIRDALTEHMIETQNEVER
DGDRQVHYLCMEFLVGRSLRNNAYNLGMLDELTAALKDMGFEMADIFEQEADPGLGNGGL
GRLAACYMDGMATTGVRGTGYSIRYEHGIFKQKIEDGKQVELPDSWLASGDVWQIPAMDE
TREVKIGGTIDTHFDENGKLIVDYHDYTPVLAVPYDMPISGYDTNNIARLRLWEAKSPIA
LDMKLFSSGEYLKAVEKHAMAETISMILYPEDNHREGQSLRLKQQYFLVSATLQDICAKH
KAKYGTLDNFAHKHVLHINDTHPTLVIPELMRILMDDNDMSWEQAWNITSQSVAYTNHTV
MPEALECWPESLVQELMPRVMQIIHEINERFCKELWSYFPGDFQKISKLAIVADGRVRMA
HLAIAGSFSINGVSALHSEILKERVFNDFYSIYSSKFCNVTNGIAHRRWLCEANPELAEL
IESKIGKGYRTHPSELQVLANYKDDKALLAQLGEIKRHNKQRLADYIKEHNGIEVSMDSI
FDVQVKRLHEYKRQMLNILHIISLYHKLKDNPDMAFVPRTYIFGSKAAPGYAVAKKTIQL
INSISNMIENDPAVRDKLKVVFIEDYKVSLAEMIMPAAEVSEQISIAGKEASGTGNMKFM
MNGALTIGTMDGANVEICEAVGRDNIFIFGMETPEAEALAASGNYEPLLIYQNDPVIHRV
LDQMIRGFGDGVDYTDIANLLLHGGNGGPADRYMSLRDFASYAEAQQRVSEAYRDAEKWN
RMALMNIANSGRFAADRSIAEYAQNIWRVKTNFAF

Enzyme Prediction     

EC	2.4.1.1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT35	95	810	5.8e-270	0.9985163204747775

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK14986	PRK14986	0.0	10	810	16	811	glycogen phosphorylase; Provisional
COG0058	GlgP	0.0	3	810	1	749	Glucan phosphorylase [Carbohydrate transport and metabolism].
PRK14985	PRK14985	0.0	5	810	4	797	maltodextrin phosphorylase; Provisional
TIGR02093	P_ylase	0.0	27	809	13	794	glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources. [Energy metabolism, Biosynthesis and degradation of polysaccharides]
cd04300	GT35_Glycogen_Phosphorylase	0.0	12	809	1	795	glycogen phosphorylase and similar proteins. This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCK80927.1	0.0	6	809	3	801
ANU40324.1	0.0	5	809	6	809
QQR06906.1	0.0	5	809	6	809
QIA32151.1	0.0	5	809	3	806
QBB67026.1	0.0	1	809	1	807

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5OX0_A	4.62e-248	22	810	37	829	GlycogenPhosphorylase in complex with CK898 [Oryctolagus cuniculus]
2GJ4_A	6.97e-248	22	810	25	817	Structureof rabbit muscle glycogen phosphorylase in complex with ligand [Oryctolagus cuniculus]
2GM9_A	7.20e-248	22	810	25	817	Structureof rabbit muscle glycogen phosphorylase in complex with thienopyrrole [Oryctolagus cuniculus],5MCB_A Glycogen phosphorylase in complex with chlorogenic acid. [Oryctolagus cuniculus],7ONF_A Chain A, Glycogen phosphorylase, muscle form [Oryctolagus cuniculus]
2FFR_A	7.20e-248	22	810	25	817	Crystallographicstudies on N-azido-beta-D-glucopyranosylamine, an inhibitor of glycogen phosphorylase: comparison with N-acetyl-beta-D-glucopyranosylamine [Oryctolagus cuniculus]
7O8E_A	8.51e-248	22	810	30	822	ChainA, Glycogen phosphorylase, muscle form [Oryctolagus cuniculus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P39123	2.25e-255	28	810	25	795	Glycogen phosphorylase OS=Bacillus subtilis (strain 168) OX=224308 GN=glgP PE=2 SV=1
Q9XTL9	1.70e-253	36	810	53	829	Glycogen phosphorylase OS=Drosophila melanogaster OX=7227 GN=GlyP PE=2 SV=2
P79334	2.92e-251	22	810	37	829	Glycogen phosphorylase, muscle form OS=Bos taurus OX=9913 GN=PYGM PE=1 SV=3
O18751	4.13e-251	22	810	37	829	Glycogen phosphorylase, muscle form OS=Ovis aries OX=9940 GN=PYGM PE=2 SV=3
Q9WUB3	4.31e-248	22	810	37	829	Glycogen phosphorylase, muscle form OS=Mus musculus OX=10090 GN=Pygm PE=1 SV=3

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000065	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001115_00501.