CAZyme Information: MGYG000002238_00114

Basic Information

• Species: CAG-110 sp900546415
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Oscillospiraceae; CAG-110; CAG-110 sp900546415
• CAZyme ID: MGYG000002238_00114
• CAZy Family: GT35
• CAZyme Description: Glycogen phosphorylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
808	MGYG000002238_2|CGC1	92797.25	6.34

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002238	1799301	MAG	Peru	South America

• Gene Location: Start: 25969; End: 28395 Strand: +

Full Sequence      

MKTPKQTKLNTTEPKRELNDVVRRLFGCELEVATEKQVYRALCTLVREKLTEQNRLFNRD
NAAKERKEVYYMSMEFLVGTSLHNNLFNLGIEEQYREALHEVGFDLDALYKMEPDAGLGN
GGLGRLASCYMDAATGMALPVTGYSIRYEFGIFRQKIVDGWQMEFPDTWLDMGDVWLRTR
EDDAVEVKFGGEVREWVDEMGRFKVAQVGYNSVIAVPHDMFISGYNSKASNRLILWSAKL
PQSFDMAAFSRGDYVRALERNAMAETISKVLYPADDHIQGKRLRLKQQYLLVSASLQSIL
KRHYKKYRTYENLADKVAIHINDTHPALCVPELMRLLIDEHDFSWDAAWDICCRTLSYTN
HTVMSEALERWNVDLFREQLPRVYSICVEINRRLIDQLSRDYPNDWGKINYMAVIANNEI
RMANLCLACCHKINGVSKLHTEILRNGIFRDYCNLTPDRFINVTNGIAYRRWLAQSNPLL
TEYLKTLIGDGFLRDANELEKLMAFHDNEEVLENLMAIKRKNKERLAALIADRNGVRVDP
DSIFDIQIKRLHEYKRQLLNVLHILYQYCQLKDHPDMPFVPHTYIFAAKASAGYIRAKQT
ISLIVAASKLINNDPDVRDKLKVVFIEDYKVSLAEIIIPAADISEQISVAGKEASGTGNM
KLMINGAVTLGTLDGANVEIHEQVGDENIFLFGLKANEVDELWQKGYNPNDFMTPELQRV
FDMLTSGVLGQHYDDLVASLLTNRFGVADPYMTVADFNDYCREQEVVAKTYTDRRKFAEM
SLVNIAKAGIFSSDRSVREYADKIWNLK

Enzyme Prediction     

EC	2.4.1.1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT35	98	807	4.7e-257	0.9970326409495549

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK14986	PRK14986	0.0	32	808	35	811	glycogen phosphorylase; Provisional
COG0058	GlgP	0.0	8	808	3	749	Glucan phosphorylase [Carbohydrate transport and metabolism].
PRK14985	PRK14985	0.0	34	808	30	797	maltodextrin phosphorylase; Provisional
TIGR02093	P_ylase	0.0	31	807	14	794	glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources. [Energy metabolism, Biosynthesis and degradation of polysaccharides]
cd04300	GT35_Glycogen_Phosphorylase	0.0	15	807	1	795	glycogen phosphorylase and similar proteins. This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AVQ97458.1	5.62e-314	10	808	6	804
AYF42955.1	5.62e-314	10	808	6	804
QCN93713.1	5.62e-314	10	808	6	804
AYF40115.1	5.62e-314	10	808	6	804
ADU27514.1	7.27e-314	4	808	37	841

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1Z8D_A	1.01e-236	10	808	24	829	ChainA, Glycogen phosphorylase, muscle form [Homo sapiens]
2FFR_A	4.23e-234	10	808	12	817	Crystallographicstudies on N-azido-beta-D-glucopyranosylamine, an inhibitor of glycogen phosphorylase: comparison with N-acetyl-beta-D-glucopyranosylamine [Oryctolagus cuniculus]
2GJ4_A	8.20e-234	10	808	12	817	Structureof rabbit muscle glycogen phosphorylase in complex with ligand [Oryctolagus cuniculus]
2GM9_A	8.48e-234	10	808	12	817	Structureof rabbit muscle glycogen phosphorylase in complex with thienopyrrole [Oryctolagus cuniculus],5MCB_A Glycogen phosphorylase in complex with chlorogenic acid. [Oryctolagus cuniculus],7ONF_A Chain A, Glycogen phosphorylase, muscle form [Oryctolagus cuniculus]
7O8E_A	9.98e-234	10	808	17	822	ChainA, Glycogen phosphorylase, muscle form [Oryctolagus cuniculus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P73511	2.96e-240	6	808	21	827	Glycogen phosphorylase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=glgP PE=3 SV=1
P0AC86	5.48e-240	27	807	30	810	Glycogen phosphorylase OS=Escherichia coli (strain K12) OX=83333 GN=glgP PE=3 SV=1
P0AC87	5.48e-240	27	807	30	810	Glycogen phosphorylase OS=Shigella flexneri OX=623 GN=glgP PE=3 SV=1
P11217	6.90e-237	10	808	24	829	Glycogen phosphorylase, muscle form OS=Homo sapiens OX=9606 GN=PYGM PE=1 SV=6
Q8HXW4	1.57e-235	10	808	24	829	Glycogen phosphorylase, muscle form OS=Macaca fascicularis OX=9541 GN=PYGM PE=2 SV=3

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000067	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002238_00114.