CAZyme Information: MGYG000003640_00116

Basic Information

• Species: Butyrivibrio_A sp900771195
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Butyrivibrio_A; Butyrivibrio_A sp900771195
• CAZyme ID: MGYG000003640_00116
• CAZy Family: GT35
• CAZyme Description: Glycogen phosphorylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
816		94506.85	5.3929

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003640	1798350	MAG	Fiji	Oceania

• Gene Location: Start: 37403; End: 39853 Strand: -

Full Sequence      

MEKMDFDKEEFKKKVVTNVKNMYRKTIDEATKQQVFQAVAYAVKDDIIDRWIATHKQIEK
QDAKTLYYMSMEFLMGRALGNNMINLTCYDEVKEALDELGFDLNAIEDEEPDAALGNGGL
GRLAACFLDSLSTLGYAAYGCGIRYRYGMFAQKIDKGYQIEIPDDWLRDGNPFEIKRPEY
AVEVRFGGYVRTYVDEKERTHFVQEGYQCVRAVPYDLPVVGYGNNIVNTLRIWDAEPIQQ
FNLDSFDKGDYQKAVEQENLAKTICEVLYPCDDHYAGKELRLKQQYFFVSASVQSAIAKY
KKNHDDVRKLYEKVCFQLNDTHPTVTVAELMRILMDQEMLTWDEAWEVTQKSCAYTNHTI
MAEALEKWPIELFSRLLPRVYQIVEEINRRFVEQIKRQYPGDQNKIAKMAIVYDGQVRMA
NLAIVAGYSVNGVAELHTEILKKQELKDFYQMMPEKFNNKTNGITQRRFLLHGNPLLAKW
VTKKIGDEWITDLSQISKLKVYADDELSRQEFMQIKYQNKLRLADYIKEHNGIDVDPRSI
FDVQVKRLHEYKRQLLNILHVMYLYNKIKLHPEMDFYPRTFIFGAKAAAGYKRAKLTIKL
INSVADVINNDKSINGKIKVVFIENYRVSNAEMIFAAADVSEQISTASKEASGTGNMKFM
LNGAMTIGTMDGANVEIVKEVGEENAVIFGLSADEVIKYQNEGGYNPQDIFNNDQEIRQV
LMQLINGFYSPDDPELFRDIYNSLLYDKRPDVYFILKDFRSYADAQTKIEDKYRDTEGWA
RSVMLNTACSGKFSSDRTIQEYVDDIWKLDKIKVDL

Enzyme Prediction     

EC	2.4.1.1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT35	95	809	3.8e-281	0.9970326409495549

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK14986	PRK14986	0.0	30	814	36	815	glycogen phosphorylase; Provisional
COG0058	GlgP	0.0	7	811	5	750	Glucan phosphorylase [Carbohydrate transport and metabolism].
PRK14985	PRK14985	0.0	6	808	5	795	maltodextrin phosphorylase; Provisional
TIGR02093	P_ylase	0.0	15	809	1	794	glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources. [Energy metabolism, Biosynthesis and degradation of polysaccharides]
cd04300	GT35_Glycogen_Phosphorylase	0.0	12	809	1	795	glycogen phosphorylase and similar proteins. This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QJU19430.1	0.0	6	816	5	817
QRP38873.1	0.0	6	816	5	817
ASN96342.1	0.0	6	816	5	817
QIX90125.1	0.0	6	816	5	817
ANU45318.1	0.0	6	816	5	817

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5OX0_A	7.36e-279	30	807	47	826	GlycogenPhosphorylase in complex with CK898 [Oryctolagus cuniculus]
2GJ4_A	7.71e-279	30	807	35	814	Structureof rabbit muscle glycogen phosphorylase in complex with ligand [Oryctolagus cuniculus]
2GM9_A	7.97e-279	30	807	35	814	Structureof rabbit muscle glycogen phosphorylase in complex with thienopyrrole [Oryctolagus cuniculus],5MCB_A Glycogen phosphorylase in complex with chlorogenic acid. [Oryctolagus cuniculus],7ONF_A Chain A, Glycogen phosphorylase, muscle form [Oryctolagus cuniculus]
7O8E_A	9.47e-279	30	807	40	819	ChainA, Glycogen phosphorylase, muscle form [Oryctolagus cuniculus]
5IKO_A	1.15e-278	30	807	50	829	Crystalstructure of human brain glycogen phosphorylase [Homo sapiens],5IKP_A Crystal structure of human brain glycogen phosphorylase bound to AMP [Homo sapiens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P79334	1.94e-278	30	807	47	826	Glycogen phosphorylase, muscle form OS=Bos taurus OX=9913 GN=PYGM PE=1 SV=3
P11217	2.75e-278	30	807	47	826	Glycogen phosphorylase, muscle form OS=Homo sapiens OX=9606 GN=PYGM PE=1 SV=6
Q9WUB3	3.89e-278	30	807	47	826	Glycogen phosphorylase, muscle form OS=Mus musculus OX=10090 GN=Pygm PE=1 SV=3
P53534	4.81e-278	30	807	47	826	Glycogen phosphorylase, brain form (Fragment) OS=Rattus norvegicus OX=10116 GN=Pygb PE=1 SV=3
O18751	5.51e-278	30	807	47	826	Glycogen phosphorylase, muscle form OS=Ovis aries OX=9940 GN=PYGM PE=2 SV=3

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000076	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003640_00116.