CAZyme Information: MGYG000000007_00684

Basic Information

• Species: Lactobacillus intestinalis
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lactobacillus; Lactobacillus intestinalis
• CAZyme ID: MGYG000000007_00684
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 1A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
780		86681.38	9.9623

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000007	2016167	Isolate	United Kingdom	Europe

• Gene Location: Start: 72695; End: 75037 Strand: -

Full Sequence      

MAENNLDPNQNMKRESRRDYHNRRRSSRLWVKIIKWFFLIVLLVVVSGIGLFAYYAKDAP
NISQNQLQSGGTSSLYTNDGKFLLSLGSEKRIYVKNDKIPQQLKDAIVSVEDKRFYKDKI
GVDPIRIVGSMLTNAKSNSIAAGGSTITQQLVKLTVFSTATSQRTLKRKAQEAWLAMRVQ
REFSKDQILEFYINKVYMNYGNYGMGTAANYYYNKPLNKLDLAQTALIAGMPNAPVAYDP
YLYPQQAKYRRDIVLKTMLENQKITKAQYNQAVNESIKHGLRPPHSNNESKIRQIDDPYI
KEVISEVKKKGFDPYNDNLKITVNIDQDAQNKLYELANNGSVPFTNDKMQIGATVVDPNT
GHVVAILGGRHLPSVQLGLDRAVQTGRSTGSSIKPVLDYAPAIQYLNWSTAKMLDDSRYV
YPGTNIQLYDWDNKYDGMMTMRKALEQSRNVPAVKTLAKVGVGRASLFARRMGIDVPANS
GLSVAIGANASSLQMAGAYGAFATMGVYHKPQFVSKIETPDGLSRNYDSPGVRVMKKSTA
YMITDMLKGVIKSGSGTRAKIDGLYQAGKTGTVKYSNEDLVRYPAYNSTPKDSWFVGYTR
SYVMGVWTGYDNLKDGTISGIGQQSAQLMYRSMMSYLMQDKENVDWQKPNSVIRARIVNN
SNPPEVATSNRSSSWQLFVRGHAPSGVSDNSANYDEEDEEADTNNQTANVTASRETTRTR
DNNGSLRQSSSTQSRITSSSSSSSSARQSSASSTSSAITSSSSSRTTQSSRSEEDNQNED

Enzyme Prediction     

EC	2.4.1.129

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	80	258	4.5e-58	0.9830508474576272

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR02074	PBP_1a_fam	0.0	90	640	1	531	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG0744	MrcB	3.69e-180	20	674	1	635	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	6.64e-128	30	657	1	738	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
pfam00912	Transgly	9.42e-73	79	259	1	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.
COG4953	PbpC	4.28e-66	33	620	3	546	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AEA32119.1	0.0	1	704	1	698
QDD70300.1	0.0	1	704	1	698
AVW86934.1	0.0	7	696	3	691
ASN47029.1	0.0	7	696	3	691
AZN76546.1	0.0	7	696	3	691

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3DWK_A	1.10e-89	67	672	2	623	ChainA, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_B Chain B, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_C Chain C, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_D Chain D, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL]
2C5W_B	7.20e-86	297	654	8	377	PENICILLIN-BINDINGPROTEIN 1A (PBP-1A) ACYL-ENZYME COMPLEX (CEFOTAXIME) FROM STREPTOCOCCUS PNEUMONIAE [Streptococcus pneumoniae R6]
2ZC5_B	8.38e-86	297	654	10	379	ChainB, Penicillin-binding protein 1A [unidentified],2ZC5_D Chain D, Penicillin-binding protein 1A [unidentified],2ZC6_B Chain B, Penicillin-binding protein 1A [unidentified],2ZC6_D Chain D, Penicillin-binding protein 1A [unidentified]
2C6W_B	9.77e-86	297	654	7	376	Penicillin-BindingProtein 1a (Pbp-1a) From Streptococcus Pneumoniae [Streptococcus pneumoniae]
2V2F_F	8.79e-85	297	654	10	379	Crystalstructure of PBP1a from drug-resistant strain 5204 from Streptococcus pneumoniae [Streptococcus pneumoniae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q04707	3.57e-164	37	654	17	642	Penicillin-binding protein 1A OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=ponA PE=1 SV=2
Q8DR59	1.00e-163	37	654	17	642	Penicillin-binding protein 1A OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=pbpA PE=1 SV=1
Q00573	2.73e-162	37	646	17	635	Penicillin-binding protein 1A (Fragment) OS=Streptococcus oralis OX=1303 GN=ponA PE=3 SV=1
P39793	7.74e-136	23	700	27	704	Penicillin-binding protein 1A/1B OS=Bacillus subtilis (strain 168) OX=224308 GN=ponA PE=1 SV=1
P38050	9.53e-79	31	635	4	595	Penicillin-binding protein 1F OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpF PE=2 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999987	0.000031	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
33	55