CAZyme Information: MGYG000000785_00044

Basic Information

• Species: Lancefieldella sp900555335
• Lineage: Bacteria; Actinobacteriota; Coriobacteriia; Coriobacteriales; Atopobiaceae; Lancefieldella; Lancefieldella sp900555335
• CAZyme ID: MGYG000000785_00044
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 2D

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
714		77097.6	4.4457

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000785	1185697	MAG	China	Asia

• Gene Location: Start: 43516; End: 45660 Strand: -

Full Sequence      

MGIRTRRARTHANTHAVGFGVAGFFGFMALLALALALSLGAAVSSWLEDLPDYNSADAYL
VAEPTRVYDSKGNDIADFYLQQRRSVTLDQISPYVIQGTIDTEDKRFYSHSGIDPWGIVR
ASVGSLFGGGEGASTITQQLVRNTVLSNEQFERSLKRKVREAYISIQMEKKFTKDQILNM
YLNTIYYGNGAYGIEAASIIYFNKHASDLTLAEAATLAGLPNAPSAYDPTVNPEAARERR
NTVLERMLRAGDITQEQYDEAVAEDLVLNPGTLTDNIGQFPYWTDYIRSLLQEDFDSDTI
LQGGLRVYTTLDPDLQKDADEAATRRIAELGNPRLGAALVSIDPQTGYIKAMVGGQDYSK
SQYNIATSSNRQMGSSFKMYTLVAALSEGMNPEIVLNGNSPMQITPTWLVRNAGNYSYGA
ITLRQGTVYSSNTVYAQVAEAIGIDKILQTCYAMGIRTQLQPYLSTTLGSQGVTPIEQCT
AFATLAAGGVRRDAVAITRIEDRNGNVIYEHKDNPVQAISPAVAAAATDVLSGVLTSGTG
RYAYNLKTFNQPVAGKTGTSDNSEDLWFCAYTPQLATVAWCGYPDSRDPVIIGGGESSTF
VTAQYVWAYYMNAALSGVARAEFPKTTETIAYKPNSYWKFVGGSANAKQNEKDDEEEEEE
ETTEEEEEDDSTTTPTTPTTPTTPATPGTGTGTGTGTGTGTGTGTGTGTGTGTP

Enzyme Prediction     

No EC number prediction in MGYG000000785_00044.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	72	247	8.3e-69	0.9830508474576272

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0744	MrcB	0.0	45	626	36	608	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	0.0	82	617	1	531	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG5009	MrcA	4.95e-169	48	648	32	754	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
PRK11636	mrcA	2.23e-95	50	618	33	788	penicillin-binding protein 1a; Provisional
pfam00912	Transgly	7.00e-88	72	248	2	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ACV51439.1	0.0	1	648	1	648
ADK67697.1	5.83e-286	1	646	1	646
QOY61037.1	3.17e-276	1	644	1	655
BBH49739.1	6.15e-270	1	647	1	648
BCV18421.1	2.48e-269	1	647	1	648

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3UDF_A	1.19e-76	58	621	14	722	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
4OON_A	3.40e-73	64	615	20	723	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
5FGZ_A	8.24e-67	81	606	154	690	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]
3VMA_A	1.14e-66	81	606	175	711	CrystalStructure of the Full-Length Transglycosylase PBP1b from Escherichia coli [Escherichia coli K-12]
3ZG8_B	7.01e-66	167	623	2	469	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O66874	1.44e-97	48	635	28	688	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1
P70997	1.96e-94	34	645	24	640	Penicillin-binding protein 2D OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpG PE=2 SV=3
O87626	1.74e-85	50	619	35	709	Penicillin-binding protein 1A OS=Neisseria flavescens OX=484 GN=mrcA PE=3 SV=1
O87579	1.26e-84	39	617	24	708	Penicillin-binding protein 1A OS=Neisseria lactamica OX=486 GN=mrcA PE=3 SV=1
P0A0Z5	1.75e-84	50	617	35	708	Penicillin-binding protein 1A OS=Neisseria meningitidis serogroup A / serotype 4A (strain DSM 15465 / Z2491) OX=122587 GN=mrcA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.918428	0.059053	0.000646	0.000556	0.000263	0.021075

TMHMM  Annotations     

start	end
21	43