CAZyme Information: MGYG000002039_00533

Basic Information

• Species: UMGS966 sp900761965
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; UMGS966; UMGS966 sp900761965
• CAZyme ID: MGYG000002039_00533
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 4

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
747		83560.12	4.2728

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002039	1613365	MAG	China	Asia

• Gene Location: Start: 5804; End: 8047 Strand: -

Full Sequence      

MAKSVRPNRLEQLPRVWYNLCVALCIGIIAACVIGCGLTVYIFKYIADEPVVDLDAAQMS
YTTILYGKTDSSSDYVEMERIHGDENRIWVSYENIPEDLINATVALEDKRFWQHQGVDWR
RTFASAVNLIIPIYEGTPGGSTLTQQVVKNITNDNDVSIVRKAKEILRAMKLEKHYSKEQ
IIEVYLNTISLGNNTAGVQAAANLYYNKDVGELNAAECASIISITQNPTKYNPFTNYDNL
VKRTQDCLYMMHQQGYLTDEEYEEAANYQLVISTETSQQVSSYTDWSWFTEQALSDVMDD
LQEQLGYTEEEAYDLLYNRGLRIYTTCDVEMQDYLEEAFNVDSNSTIFPAVVNETYPEGA
FVVLDLNGQIKALAGSNRDKEGARVFNRARDAVRHPGSTIKPIASYALAVENDIIHFSSL
VLDSPITLVENGVSRQWPVNFYGTYLGNITVDLAIRRSTNTIPVKLQQILSPQVSFNFLK
NKLGMETLVESRTENGKVYSDINIAPMALGEMTDGVTPLSMAGAYQIFGNGGYYTKPYSY
TKVTDASGNVILEQKPVPSQVISGETATVMNHLLQGVTTGSQGTGTTAKFSAMPVAGKTG
TSDNDNNQWFIGVTPYYVGVCWLGYDIPERISYYSYAPPIIFKNIMGPIHQNLPVIQFRD
NTNVEVLEFCTITGDLAGEDCTTTSYGWYKPSNIPDVCNGDHDVNKTEEDQQDEEDPQDD
EESSSQRDSERSSRNSRDDRQSSGEEE

Enzyme Prediction     

No EC number prediction in MGYG000002039_00533.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	79	251	6.2e-59	0.96045197740113

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR02074	PBP_1a_fam	6.25e-154	86	652	1	531	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG0744	MrcB	2.15e-151	26	659	21	606	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	3.10e-114	22	691	5	760	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
pfam00912	Transgly	4.48e-70	83	252	9	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.
COG4953	PbpC	1.27e-58	83	624	54	534	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCI59848.1	9.18e-173	3	702	17	740
QNK41697.1	2.14e-166	28	710	42	716
QAT49292.1	1.87e-159	29	708	43	715
CDZ23183.1	4.33e-159	26	690	40	696
QEY34222.1	2.36e-157	29	702	38	704

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7U4H_A	4.33e-64	48	626	4	736	ChainA, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX],7U4H_B Chain B, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX]
4OON_A	5.81e-55	83	699	35	772	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
3ZG8_B	5.01e-54	171	633	2	440	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]
3DWK_A	1.87e-51	90	649	30	588	ChainA, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_B Chain B, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_C Chain C, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_D Chain D, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL]
3UDF_A	8.54e-50	82	659	34	725	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A5I6G4	7.44e-102	63	676	57	670	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) OX=441771 GN=pbpA PE=3 SV=1
A7FY32	7.44e-102	63	676	57	670	Penicillin-binding protein 1A OS=Clostridium botulinum (strain ATCC 19397 / Type A) OX=441770 GN=pbpA PE=3 SV=1
A7GHV1	1.90e-101	63	676	57	670	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) OX=441772 GN=pbpA PE=3 SV=1
Q8XJ01	3.65e-99	17	664	30	677	Penicillin-binding protein 1A OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=pbpA PE=3 SV=1
Q0TNZ8	8.14e-99	17	699	30	722	Penicillin-binding protein 1A OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=pbpA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000036	0.000002	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
20	42