CAZyme Information: MGYG000002178_00158

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; CAG-617
• CAZyme ID: MGYG000002178_00158
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 1A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
846		94513.07	7.2548

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002178	2197502	MAG	Spain	Europe

• Gene Location: Start: 688; End: 3228 Strand: -

Full Sequence      

MTTRNKGFWAKIVAFFSAIGRGFRRCWRWYVGQYKGRPWYRKLLAAFLSFVAFVLFYIFA
VSVNLFWLFGKSPSLNEIMHPKTAAASEIYSADGKLMGKFFSENRSPVPYDSINPVFFQA
LISTEDERFYRHHGIDFQGIFAALKDAMQGKARGASTITQQLVKNMFRVRTEYSTGLLGK
IPGLDILIMKSKEMILAVGLEMFYSKQEILNMYANTVDFGSNAYGIKTAAQTYFSTTPYQ
LKAEEAAVLVGLLKATSYYNPRFNPKNSLRRRNTVLENMRTHGYLTAAACDSLQAVPIRL
KFKVESAYDGKALYFRQAVADYLEEHCPELDPYSDGLKIYTTLDSRMQQYAEEAMREQMK
VVQRNFDRHWGTRDPWVDERGNVIPGFIEDIARRTDTYKMLSAKYDGNADSINYYLNLPH
RVKVFSYDGEREMEMSTMDSIRYMVHFMHAGFVAMEPATGYVRAWVGDIDFSSWKYDKVK
AMRQPGSTFKLFVYATAMKQGLTPSDRRRDEYIRMEVYDKQKGENVLWQPHNADGRFSNA
DVPLRSAFARSINTIAVKLGQEVGISNVARTAQDMGIRSPLDETPSLALGSSDVNLLEMV
NAYATVANDGLHVEPVLVAKVVDAEGDVVYEADPDEEQALPYRAAFFMQQLLEAGTHDAG
GTSQSLNRYVRPYADRLDFGGKTGTSNNHSDAWFIGVTPALVGGAWVGGEYRSIHFRTGA
LGQGSRTALPIVGLFMEKVLADPSLASRYLRKYGAPKEDIEPSLYRVSYTPPAEPADSLD
ADSTALAADSLMGTPHAGAAGADSLALPAASQGEAVSGNQGTPDAGRHHDGAATPQRERP
VDDLFN

Enzyme Prediction     

No EC number prediction in MGYG000002178_00158.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	93	280	1.3e-60	0.9943502824858758

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR02074	PBP_1a_fam	7.73e-157	104	742	1	531	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG0744	MrcB	2.47e-156	35	744	5	601	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	1.55e-147	39	789	1	781	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
PRK11636	mrcA	1.88e-84	88	762	49	795	penicillin-binding protein 1a; Provisional
TIGR02071	PBP_1b	8.83e-84	93	708	132	656	penicillin-binding protein 1B. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of a particular bifunctional transglycosylase/transpeptidase in E. coli and other Proteobacteria, designated penicillin-binding protein 1B. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ALO49868.1	0.0	14	784	7	779
EFC71818.1	0.0	11	784	4	780
QUB46878.1	0.0	24	783	17	779
VEH15546.1	0.0	11	774	2	781
QYR10875.1	0.0	24	768	14	764

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3UDF_A	4.10e-71	88	740	22	716	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
4OON_A	1.93e-65	88	742	22	725	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
5U2G_A	2.31e-58	70	634	5	568	2.6Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20],5U2G_B 2.6 Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20]
5FGZ_A	5.42e-47	103	708	154	667	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]
3VMA_A	6.51e-47	103	708	175	688	CrystalStructure of the Full-Length Transglycosylase PBP1b from Escherichia coli [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O66874	4.45e-81	73	739	33	667	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1
P0A0Z6	6.08e-78	41	731	3	697	Penicillin-binding protein 1A OS=Neisseria meningitidis serogroup B (strain MC58) OX=122586 GN=mrcA PE=3 SV=1
P0A0Z5	6.08e-78	41	731	3	697	Penicillin-binding protein 1A OS=Neisseria meningitidis serogroup A / serotype 4A (strain DSM 15465 / Z2491) OX=122587 GN=mrcA PE=3 SV=1
O87626	8.39e-78	69	731	32	696	Penicillin-binding protein 1A OS=Neisseria flavescens OX=484 GN=mrcA PE=3 SV=1
Q5FAC7	1.60e-77	43	731	6	697	Penicillin-binding protein 1A OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) OX=242231 GN=mrcA PE=3 SV=3

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999451	0.000557	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
47	69