CAZyme Information: MGYG000002686_00240

Basic Information

• Lineage: Bacteria; Actinobacteriota; Coriobacteriia; Coriobacteriales; QAMH01; QAMH01
• CAZyme ID: MGYG000002686_00240
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
720		77626.31	4.5205

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002686	1530597	MAG	Canada	North America

• Gene Location: Start: 430; End: 2592 Strand: +

Full Sequence      

MCSHRKETELPSSSRNRKRREKSQRRSVNLLTILGCIAGAFCLVVVIGCAVGATWLMGLP
DYSAINTYANTGITTIYANDRQTVLAKIYLENRIEVTKDEVAEYVLEGTIATEDERFYEH
GGIDPIGIARAVLVNAASGGTSEGASTITQQLVRNTVLLDEMTDRSIRRKVREMYIASQV
ERQYTKDEILMMYINVVNYGDGCYGIEAASRDHFGKHADELTLSEAALLIGIPQSPTAYN
PREHMDKAMARRAVVLQRMLSNGYITQDEYDEALNDTPVLSNETMEDETISDIAPYFIDY
VRKQLDEDERFPATIIAHGGLSVYTTLDPALQKDANDAIQQAVRYNDENFDAALVSVDPD
NGQIVAMVGGRDYETNKFNLATQMSRQAGSAFKTFTLLSALNEGMDPDNTYIESASPVEI
GKDWTVSNSEGHGYGPMSVADATRSSVNTVFAQIVHVVGAQKTIDAAKACGIESPLVQDE
TVTLGTSGVNPLEMANAYATIANGGYYHETYAVVEIVDASGKAVYTHEEVPAKRALSAAV
AQKATDILETVISSGTGTGASLSNGQPCAGKTGTSEHGRDLWFCGFTPQYSTAVWAGYRT
ERETGLYGGSICAPIWRSFMNAALAGKPIEQFPTTSEKITYKPAKTWDFTKDITIIGGDE
EDPKPSESSSSSSSSSASSSSAASGSASSSAKPDGGETPKPDPKPEPGGDDKPEPATQSS

Enzyme Prediction     

No EC number prediction in MGYG000002686_00240.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	83	259	3.6e-65	0.9774011299435028

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0744	MrcB	0.0	20	635	4	608	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	0.0	92	626	1	531	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG5009	MrcA	1.16e-162	37	632	4	727	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
TIGR02071	PBP_1b	2.13e-109	91	652	145	711	penicillin-binding protein 1B. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of a particular bifunctional transglycosylase/transpeptidase in E. coli and other Proteobacteria, designated penicillin-binding protein 1B. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
PRK11636	mrcA	5.29e-89	93	633	66	794	penicillin-binding protein 1a; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADK67697.1	7.38e-172	55	664	46	649
ACV51439.1	2.92e-170	40	649	32	640
SDR66234.1	1.42e-168	53	650	44	641
ACU93960.1	1.46e-167	13	632	2	616
BCV18421.1	4.69e-163	55	651	46	643

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3UDF_A	8.73e-72	76	628	23	720	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
4OON_A	1.49e-63	100	629	46	728	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
5U2G_A	1.11e-61	59	525	7	564	2.6Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20],5U2G_B 2.6 Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20]
3ZG8_B	3.64e-61	179	603	2	438	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]
3DWK_A	2.51e-58	96	605	30	564	ChainA, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_B Chain B, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_C Chain C, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_D Chain D, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O66874	4.21e-90	59	639	30	683	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1
Q68VU2	5.77e-86	41	628	12	721	Penicillin-binding protein 1A OS=Rickettsia typhi (strain ATCC VR-144 / Wilmington) OX=257363 GN=mrcA PE=3 SV=1
Q9ZCE9	3.12e-85	41	628	12	721	Penicillin-binding protein 1A OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=mrcA PE=3 SV=1
P70997	1.18e-83	74	632	55	616	Penicillin-binding protein 2D OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpG PE=2 SV=3
Q92G78	1.24e-82	41	628	12	721	Penicillin-binding protein 1A OS=Rickettsia conorii (strain ATCC VR-613 / Malish 7) OX=272944 GN=mrcA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999862	0.000173	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
27	49