CAZyme Information: MGYG000003140_00243

Basic Information

• Species: Pauljensenia sp000758755
• Lineage: Bacteria; Actinobacteriota; Actinomycetia; Actinomycetales; Actinomycetaceae; Pauljensenia; Pauljensenia sp000758755
• CAZyme ID: MGYG000003140_00243
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
801		84635.97	6.9956

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003140	1808392	MAG	United States	North America

• Gene Location: Start: 20514; End: 22919 Strand: +

Full Sequence      

MAPSQANDDQGRRALPSRAEATRRRLAQQARREGEGTIPQTPASSAPKRTSAVARVSRGS
SSGAAASSSGSRRASTRAASSGSTSRTGSTRPASSRTAASGKGGGKKKGPAKGKKKRSLA
ARIARGAFYTFLGLIIAGFGTFLIAYASLKIPQADQVALAQTTTVYYADGTTEMGRMSQV
NRKIIDSSTLPDYVSHAVVASEDRTFYTNSGVDLKGIGRALVNNIRGGARQGASTLTQQY
VERYYVGETTGYVGKAKEAILALKINREQTKDEIIGNYLNTIYFGRGAYGIEAASQAYFG
HEAKDLTLSEAAMIAGIIPAPSAWDPAVDEEKARSRWQRVINLMVEDGWISQADANAAEF
PKTIDPDTLGGPTMEGPTGYLMEQVRTELAAEGFTADQLATGGYRIISTIDKDKQDQAVA
AAEYMYTVRGWNEDSMHVALTSLDPATGEIVAEYGGKDYLTRQQNAATQDIMAAGSTFKP
FALVAHAEQGGSIYDVYDGSSPKTFSGMSRAVSNDSGVSWGRQNLVNATKNSINTVFVGL
NQEVGPATTKKVAIDLGIPEDTSGLDDSLLNVLGFAAPHNIDLAHAYSTIASGGKRTTPH
IVREVQNSDGSKVFKTNVDPEQVFDTKTMSTVLPALEAVTASGGTAEAAAALKQDTGGKT
GTSEDQKTAQFVGFTPELVTAVSMYQLDENGNPVSLTNIGGLGQFHGGDWPVTVWMRYMK
QATANQTKLHFDWYTRQSATPVAPAPQVSTTPTPSEETPTPTPEATESTPGSEVQPTQTP
TAQNTGAAGSEGGGPSRAEGR

Enzyme Prediction     

No EC number prediction in MGYG000003140_00243.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	173	345	2.2e-61	0.9774011299435028

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0744	MrcB	1.73e-138	117	786	9	661	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	7.33e-132	181	725	1	531	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG5009	MrcA	3.41e-101	128	794	9	795	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
pfam00912	Transgly	2.93e-74	172	345	3	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.
PRK09506	mrcB	3.89e-46	182	729	211	749	bifunctional glycosyl transferase/transpeptidase; Reviewed

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QYB16859.1	4.57e-279	114	794	71	762
AOS47583.1	5.36e-278	115	738	73	700
SDT85356.1	4.88e-277	113	756	63	700
QWW20550.1	1.12e-274	97	753	80	736
QCT34577.1	1.53e-273	107	780	53	714

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3ZG8_B	2.90e-45	264	702	2	449	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]
3UDF_A	3.48e-42	185	722	42	715	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
3ZG7_B	7.94e-42	264	682	2	428	CrystalStructure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the apo form [Listeria monocytogenes]
5FGZ_A	1.33e-38	182	727	156	694	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]
3VMA_A	1.49e-38	182	727	177	715	CrystalStructure of the Full-Length Transglycosylase PBP1b from Escherichia coli [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A0R7G2	1.57e-79	123	682	71	647	Penicillin-binding protein 1A OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) OX=246196 GN=ponA1 PE=3 SV=1
P71707	2.95e-79	123	727	133	753	Penicillin-binding protein 1A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ponA1 PE=1 SV=3
O86088	2.21e-57	122	722	4	705	Penicillin-binding protein 1A OS=Neisseria cinerea OX=483 GN=mrcA PE=3 SV=1
P0A0Z6	3.52e-56	139	722	19	705	Penicillin-binding protein 1A OS=Neisseria meningitidis serogroup B (strain MC58) OX=122586 GN=mrcA PE=3 SV=1
P0A0Z5	3.52e-56	139	722	19	705	Penicillin-binding protein 1A OS=Neisseria meningitidis serogroup A / serotype 4A (strain DSM 15465 / Z2491) OX=122587 GN=mrcA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000048	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
127	149