CAZyme Information: MGYG000003423_00066

Basic Information

• Species: W3P20-009 sp900766825
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; W3P20-009; W3P20-009; W3P20-009 sp900766825
• CAZyme ID: MGYG000003423_00066
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 1A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
820	MGYG000003423_4|CGC1	92325.59	6.4268

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003423	2824109	MAG	Fiji	Oceania

• Gene Location: Start: 6022; End: 8484 Strand: -

Full Sequence      

MLKRASCAQSPANENSELMKPEHKKYVIGFWAIFGSMVLAVVIFFSLLSAGVLGYMPSFK
ELENPKSNLASEILSADGKPLGRFYIENRSYVDYEQLSPNLVHALIATEDERFYEHSGID
FKSLFRVLFKTVLGGNESSGGGSTVTQQLAKMLFHDQASSLSERLLQKLNEWVIAVKLER
AYTKEEIIAMYFNKVGYVYDAYGIESAAYTFFGTKAQDLTIEQAAVLVGMLKNPSYFNPI
RHAERTEQRRNVVLSQMVKAKYLTQQECDSLQAIPMDCSKFKREDHKDGTAPYLREFLRL
TLKAHKPERKNYASWQDQEFRDDSIQWENNPLYGWIDKNPKADGQLYDIYRDGLKIHTTI
DSRMQAYAEQAVTEHIGHTLQPLFFKDKKGAKYAPYSRDITEEQFNSMVGRAVRNSDRWR
IMRKSGMSETEAMAEMKKPVDMRVFDWKAEALERDTVMSPYDSVVYHKHFLRSGFMALDP
HTGGVKAYVGGINFRYFQYDMVGKGRRQVGSTIKPFVYTTIFRDGHTPCDLVPNSPQTFT
VPAEGGTTKTWTPRNSGDERAGEMVSLKWGLANSNNNVTGWAMKQTSPEQVANLIHALGV
ESHIDPVPSLFLGTSDVKLLEMVGAYATYVNKGVHIDPIFVTEIEDKYGNVIARFGRRER
EVIDEQTAYMMINLLEGVVNQGTGRRLRGPTYRLTCQMGGKTGTTQNMSDGWFMAVLPNL
VCGVWVGGEERGIHFNYMSVGQASNVAIPVFGRFILKVYDDATLTQVRPTDIFEAPSTIN
FSLDCSDKNINADGGDTYADSGSASGGDEECEESGGIFDF

Enzyme Prediction     

No EC number prediction in MGYG000003423_00066.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	77	258	1.7e-63	0.9943502824858758

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0744	MrcB	1.17e-147	24	816	11	644	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	1.44e-142	38	810	8	756	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	8.24e-142	88	754	1	524	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
PRK11636	mrcA	3.24e-73	72	727	49	742	penicillin-binding protein 1a; Provisional
pfam00912	Transgly	1.49e-69	77	258	1	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ASB49350.1	1.93e-309	24	788	8	762
BAX80294.1	7.72e-288	24	787	22	774
QRO50642.1	3.21e-286	30	787	12	760
QUT47776.1	1.05e-285	24	776	7	751
QKH98213.1	1.21e-284	24	776	7	751

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3UDF_A	5.86e-68	72	730	22	690	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
4OON_A	1.83e-65	62	772	12	737	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
5U2G_A	8.60e-52	56	649	7	560	2.6Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20],5U2G_B 2.6 Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20]
7U4H_A	2.55e-36	86	736	37	743	ChainA, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX],7U4H_B Chain B, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX]
5FGZ_A	1.88e-35	87	786	154	720	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O66874	1.14e-73	24	776	2	679	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1
O87579	1.10e-68	30	757	10	707	Penicillin-binding protein 1A OS=Neisseria lactamica OX=486 GN=mrcA PE=3 SV=1
P0A0Z6	1.01e-67	56	757	35	707	Penicillin-binding protein 1A OS=Neisseria meningitidis serogroup B (strain MC58) OX=122586 GN=mrcA PE=3 SV=1
P0A0Z5	1.01e-67	56	757	35	707	Penicillin-binding protein 1A OS=Neisseria meningitidis serogroup A / serotype 4A (strain DSM 15465 / Z2491) OX=122587 GN=mrcA PE=3 SV=1
O87626	4.92e-67	56	755	35	701	Penicillin-binding protein 1A OS=Neisseria flavescens OX=484 GN=mrcA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999983	0.000038	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
27	49