CAZyme Information: MGYG000003735_00212

Basic Information

• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Burkholderiales; Neisseriaceae; QFNR01
• CAZyme ID: MGYG000003735_00212
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 1A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
820		89156.68	9.9275

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003735	2323542	MAG	Canada	North America

• Gene Location: Start: 14648; End: 17110 Strand: +

Full Sequence      

MFKRFIKNTFLLVLGVCFGAALLIAGAVCVLVLKTYPELPSLSEVASYRPKIPLRIYTSD
GKLIGVYGEERRNFTRIEEFPQVLKNAVVATEDRRFYTHSGVDFKGIARAAYSNYVAGGI
RSGASTITQQVARNFYLNPERSYRRKFKEALLAHKIERALSKDQILELYFNQIFLGQRSY
GFAAAAQAYYGKPVQQLSLAEAAMLAGLPQAPSTNNPFSNPKGAKRRQLTVLQNMVEEGM
ITPAQRDEAAAEEIKYKRQVIDVDPNTLYVAEMTRQLMFDRYGEKAYTQGYRVYTTVDSK
AQAKATEALRKTLDGNTRNGPYGGAIGQFDLDAGGNQDDVGMARSYLTRFHKAGDLLPAV
VLYASPTLMRVAIMGQEGTQQLGPSQFSIARNVIDKPGMKRAVRRGSVVRVRNAGKPDAP
RWVIAQEPELQGALVSLDARTGAIQALVGGYDFYAKEFNRATQAMRQPGSTLKPFIYSAA
LQRGFTASTLVNDAPLTIPHVGKGGRDWSPKNSGNRYSGMITLRAALVASRNVVSVRLLL
SMGIDYAADYLQRFGFPASQLPKSYSLALGTASVTPLQMAEAYAVFANGGYKTSSYIIDR
IQDDKGRIIAQTKPLIAGANAHLVIDPRNAFIMTTIMQDITRVGTAARASSLKRKDIAGK
TGTTNDQKDAWFVGFTPKTVTAVYIGYDKPRNMGSIGFGGRIALPVWIDYMGWALKDVPQ
YQFEMPKGVIKQGGEYYLAERLRPAADLNLDNSASEPGDDADVYSLEDDLPSGESPIYPA
GATPGNLGSMGGPLGSSGSGGRAQGSNLGSMGGSAADALF

Enzyme Prediction     

No EC number prediction in MGYG000003735_00212.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	60	236	1.4e-67	0.9943502824858758

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK11636	mrcA	0.0	4	730	2	800	penicillin-binding protein 1a; Provisional
COG0744	MrcB	0.0	1	776	7	654	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	0.0	3	776	2	779	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	0.0	71	717	1	531	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG4953	PbpC	9.16e-90	21	711	6	558	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QMT39544.1	2.08e-303	6	762	2	751
AVR80117.1	3.78e-303	6	757	2	747
AVI44852.1	3.78e-303	6	757	2	747
AZR59551.1	5.33e-303	1	779	1	761
QTM23119.1	5.35e-303	6	757	2	747

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4OON_A	1.76e-175	51	786	18	782	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
3UDF_A	1.64e-157	53	719	20	720	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
5U2G_A	5.67e-147	38	732	6	775	2.6Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20],5U2G_B 2.6 Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20]
7U4H_A	1.03e-68	70	707	38	753	ChainA, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX],7U4H_B Chain B, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX]
5FGZ_A	1.44e-67	70	704	154	685	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O87626	4.16e-298	6	757	2	747	Penicillin-binding protein 1A OS=Neisseria flavescens OX=484 GN=mrcA PE=3 SV=1
O87579	7.24e-294	1	757	1	748	Penicillin-binding protein 1A OS=Neisseria lactamica OX=486 GN=mrcA PE=3 SV=1
O86088	2.36e-292	1	757	1	750	Penicillin-binding protein 1A OS=Neisseria cinerea OX=483 GN=mrcA PE=3 SV=1
P0A0Z6	2.71e-291	1	757	1	748	Penicillin-binding protein 1A OS=Neisseria meningitidis serogroup B (strain MC58) OX=122586 GN=mrcA PE=3 SV=1
P0A0Z5	2.71e-291	1	757	1	748	Penicillin-binding protein 1A OS=Neisseria meningitidis serogroup A / serotype 4A (strain DSM 15465 / Z2491) OX=122587 GN=mrcA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.995751	0.002879	0.000446	0.000021	0.000011	0.000915

TMHMM  Annotations     

start	end
11	33