CAZyme Information: MGYG000003775_00002

Basic Information

• Species: Ezakiella massiliensis
• Lineage: Bacteria; Firmicutes_A; Clostridia; Tissierellales; Peptoniphilaceae; Ezakiella; Ezakiella massiliensis
• CAZyme ID: MGYG000003775_00002
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 1A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
986		110111.04	4.6059

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003775	1421456	MAG	Canada	North America

• Gene Location: Start: 3432; End: 6392 Strand: -

Full Sequence      

MPHRNKVLTPGKLIVYIIYIALIVSIILGSLLALSVVSIMKEVDEVDPNIIANNLTETSK
IYDLDGNLIEKVETAEYRTVIGIEQMPKQLIQAFLAIEDERFYEHDGVDLIGIMASFRDA
FSSGQLRGGSTITQQLVKNTYLTNERKLKRKIHEITMSIDLEKKMSKDQTIEAYLNTVSL
GQNAYGVEEAAQTYFSKSAKDLNLVECAMLAGVVKSPTKYPPYKRVSPEHFDPNTMEKVG
FIELLNQNYILVYNEENEKRTDVVLSQMLKYGKITQEEYNQAMSVDRKTLIKPGETKTTD
ITNYFTDYVLKQVEQDLMDKYGWSKKIAQNKIFYGGIQIYSTINVGYQKIMEDAYKNFVE
ILFGNTNNMRAPIILDTTSDAYANLVDSNGNFIYLAKDNLIVQNSDDIYLAPGEWRYDEN
GNLCIKSKKLKAYNSNIDIQDYYTIDEKKTLVTHIVGSLPIAKDDFWVENDEIVISSKAI
ASNDSLFKNKDDYLHIDGNQVYRNKKGVPQPQSAMIIIDYHTGHILAMIGGRDVDGNRFL
NRATDSHRQPGSTIKPVSVYLPALENGYTAASPIDDVPIVVNGKIWPNNWYLGFRGIHSL
RYAVEQSINVSAVKVLNAMGTNKVVPYLESMGIIDTEHPDRDSFVSASENKEVNDENASS
LALGGMTNGLSPLALTAAFGTIANDGVYLKPTSYTKVIDNDGTTLLEYTENPVQAAKPQT
AYVMKDILRTVVTRGLGTRAQIPGQVVAGKTGTTQYTADLWFSGFTDYYAASTWIGSDSP
KITMKQNSMIAAQLFQYVMSQVHEGLPSVTQFKEPEGIVRASVCTMSGKSPNEFCTGDPR
GTVITEIFVKGTEPTEACDAHVEVEICTESNMLANEFCPEETREKVVRVQTTPPYDPESH
GGVIPSDMQYRVPSKTCDIHNESNTGESEIIDILNPGGDDEFDINDFLNNNNNKNENNTD
TPDTNHENQDENEDQKEPPGTIDLLQ

Enzyme Prediction     

No EC number prediction in MGYG000003775_00002.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	65	268	5.7e-62	0.9887005649717514

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG5009	MrcA	3.93e-161	12	863	8	773	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	1.00e-158	77	805	1	531	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG0744	MrcB	7.67e-152	12	855	9	650	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02071	PBP_1b	8.63e-83	59	837	115	714	penicillin-binding protein 1B. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of a particular bifunctional transglycosylase/transpeptidase in E. coli and other Proteobacteria, designated penicillin-binding protein 1B. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
PRK11636	mrcA	1.13e-78	60	856	49	830	penicillin-binding protein 1a; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SHD77321.1	9.09e-309	41	937	44	943
AVM66314.1	2.66e-302	45	941	49	962
QQY80508.1	5.29e-301	41	927	52	941
QAT62113.1	1.32e-297	41	939	44	943
QIB26572.1	1.40e-260	41	924	44	934

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7U4H_A	1.53e-181	48	856	6	809	ChainA, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX],7U4H_B Chain B, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX]
4OON_A	4.01e-70	60	830	22	749	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
3UDF_A	9.16e-56	52	808	14	721	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
5U2G_A	1.62e-50	55	855	18	801	2.6Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20],5U2G_B 2.6 Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20]
3ZG8_B	1.58e-44	160	800	2	458	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7GHV1	2.57e-106	57	865	55	706	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) OX=441772 GN=pbpA PE=3 SV=1
A5I6G4	1.03e-105	57	865	55	706	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) OX=441771 GN=pbpA PE=3 SV=1
A7FY32	1.03e-105	57	865	55	706	Penicillin-binding protein 1A OS=Clostridium botulinum (strain ATCC 19397 / Type A) OX=441770 GN=pbpA PE=3 SV=1
Q0SRL7	4.25e-100	45	861	59	724	Penicillin-binding protein 1A OS=Clostridium perfringens (strain SM101 / Type A) OX=289380 GN=pbpA PE=3 SV=1
Q891X1	5.81e-100	54	864	56	716	Penicillin-binding protein 1A OS=Clostridium tetani (strain Massachusetts / E88) OX=212717 GN=pbpA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.966823	0.018606	0.000149	0.000138	0.000069	0.014241

TMHMM  Annotations     

start	end
13	35