CAZyme Information: MGYG000004261_00165

Basic Information

• Species: UMGS1449 sp900551925
• Lineage: Bacteria; Firmicutes; Bacilli; RF39; UBA660; UMGS1449; UMGS1449 sp900551925
• CAZyme ID: MGYG000004261_00165
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 2D

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
649		73408.66	4.4673

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004261	785527	MAG	China	Asia

• Gene Location: Start: 2975; End: 4924 Strand: +

Full Sequence      

MKKGQKIVKIGVITLLILIGIFILFNLIGFLYAYITPKLDISSANAFSVYDNKGSLVFQG
NGNDSWVSLDKMNDNIINATLSVEDKNFYRHWGFDFPRIGAAFLANISSGEIVQGASTIT
QQYAKNLFLDFEQSWTRKWKEMWLTYELEAHYTKDEILEGYLNSINYGHGMYGISNASKY
YFDKEVGELSLAEASILAGIPNSPSDYSPIDNYEMAKKRQKVVLERMYNNGYITKKEMDI
AYKTKLNFKNDSFDNELTSLIYYNDAVMNELEEIGTIPSSYLDTGNIKIYTTLDIEAQKS
LEAGLSEIAINDEIETSKIMMDPDDGAIIALIGGLNYGESQFNRVINSYRQPGSTVKPFL
YYRALENGFTPSTTFFSKETSFYFDNKETYTPANSGNIYANKEISLAAAMAYSDNIYAVK
THLFLGEFELYNTLRKVGITSKMEKSPSLTLGTYEVSIIELARAYSALANSGHKVEPHLI
TKVVDKNDNVLYEYKEKEEEVVLNSDITFMISELLTTTYDTNLIDYTYPTCINMVDRITN
KYAIKSGSTDTDAWVIGYTPEVVFASWAGYDDNKEISTEVVSANKDSWITSMEKYFETND
ATWYDVPNNVVGVLVNPITGNVVSNSNEKKKILYYLKGTEPYIKEDSDD

Enzyme Prediction     

No EC number prediction in MGYG000004261_00165.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	61	228	2.9e-66	0.943502824858757

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR02074	PBP_1a_fam	1.11e-171	66	597	4	530	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG0744	MrcB	5.79e-166	27	573	29	574	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	8.32e-133	27	646	22	769	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
pfam00912	Transgly	5.60e-83	63	228	12	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.
COG4953	PbpC	1.28e-71	46	574	36	539	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QYF83258.1	6.34e-176	2	641	10	653
QNK49558.1	5.07e-175	2	641	10	653
QOR66557.1	1.08e-174	17	641	24	653
AZV45469.1	1.26e-174	2	641	10	653
AZV62027.1	2.03e-174	2	641	10	653

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3ZG8_B	1.96e-58	147	587	2	451	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]
3UDF_A	8.57e-56	75	603	50	723	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
3ZG7_B	1.29e-55	147	587	2	451	CrystalStructure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the apo form [Listeria monocytogenes]
4OON_A	4.51e-55	49	620	23	747	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
3DWK_A	8.56e-55	49	575	10	562	ChainA, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_B Chain B, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_C Chain C, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_D Chain D, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P70997	7.17e-153	49	641	57	653	Penicillin-binding protein 2D OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpG PE=2 SV=3
O66874	1.30e-82	5	643	1	714	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1
P38050	1.09e-69	67	571	70	571	Penicillin-binding protein 1F OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpF PE=2 SV=2
P39793	6.43e-67	2	641	23	691	Penicillin-binding protein 1A/1B OS=Bacillus subtilis (strain 168) OX=224308 GN=ponA PE=1 SV=1
Q68VU2	1.12e-65	66	623	69	744	Penicillin-binding protein 1A OS=Rickettsia typhi (strain ATCC VR-144 / Wilmington) OX=257363 GN=mrcA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.800022	0.190311	0.001049	0.000937	0.000485	0.007219

TMHMM  Annotations     

start	end
12	34