CAZyme Information: MGYG000004546_00802

Basic Information

• Species: CAG-460 sp900751815
• Lineage: Bacteria; Firmicutes; Bacilli; RF39; UBA660; CAG-460; CAG-460 sp900751815
• CAZyme ID: MGYG000004546_00802
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 2D

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
652		73716	4.3422

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004546	1057207	MAG	France	Europe

• Gene Location: Start: 96; End: 2054 Strand: +

Full Sequence      

MKKVRFLFKLMLFGFISFIVIIIGLYTYAYLSPKLDIKTSGSLYLYDSNNELVYQGSRNN
EWANLEDISDYLIDATIAVEDKNFYKHHGFDYLRIIKAMYLNIKSGTIVQGASTISQQYI
KNLYLDFDQTWERKIEEAFLTLELEVHYEKDEILEGYLNTINYGQGNMGIVSAAEYYFNK
KPSELSLEEAIMLAGISKNPARYNPVSDYDACIARAKVVAKSMLNNEYIDEDTYNSLFQD
KIEIYGQNKTNNLQMLMYYQDAVMDELASISEIPESLIDAGGLKIYTTLDIDMQANLEEN
ILENKPDDEIQVASVVVDPNTGAIRALTGGMDYAKSQYNRALESKRQVGSTMKPFLYYAA
LENNMTMSSTFSSVPTTFTLSNGQTYSPQNADSLYADKEITMAAALALSDNIYAVKTNLF
LGVDKMIEVAHRTGIEADLDEVASLPLGTSEINLLDFATGYTTFATGGYKKDLYFIERIE
DLEGNVLYEKEHEKKLVLNPNYTYILNEMMNTTSNDAYVDYTTPTALTVSSGFTNKYAIK
TGSTNTDFWIVGYNKDALVMTWMGYDDNKAVTSRVRTSSKKAWTKTIEYALKDIDTSWYE
TPENVVAIPLDAVTGKVTNDNNKVALYYYVKGSEPSVLNEQYVSSENEKNTE

Enzyme Prediction     

No EC number prediction in MGYG000004546_00802.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	56	223	5e-62	0.943502824858757

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0744	MrcB	3.44e-176	1	630	9	630	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	1.61e-173	61	593	3	531	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG5009	MrcA	1.19e-131	1	636	2	765	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
TIGR02071	PBP_1b	1.94e-100	65	568	152	660	penicillin-binding protein 1B. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of a particular bifunctional transglycosylase/transpeptidase in E. coli and other Proteobacteria, designated penicillin-binding protein 1B. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
PRK11636	mrcA	8.09e-84	1	636	1	829	penicillin-binding protein 1a; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AMC08260.1	1.83e-165	20	630	29	644
QQX26484.1	1.81e-164	2	647	11	665
QJS18329.1	5.93e-162	19	636	23	646
QQZ09247.1	7.25e-161	19	647	31	665
QJX64342.1	3.53e-158	2	636	13	653

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5FGZ_A	6.90e-62	70	569	166	672	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]
3VMA_A	9.20e-62	70	569	187	693	CrystalStructure of the Full-Length Transglycosylase PBP1b from Escherichia coli [Escherichia coli K-12]
3FWL_A	5.39e-58	70	569	170	676	CrystalStructure of the Full-Length Transglycosylase PBP1b from Escherichia coli [Escherichia coli]
3UDF_A	7.20e-57	43	598	21	723	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
4OON_A	3.90e-56	67	640	46	773	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P70997	1.35e-134	32	636	46	654	Penicillin-binding protein 2D OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpG PE=2 SV=3
O66874	2.61e-67	56	637	59	714	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1
P39793	3.63e-67	32	649	63	705	Penicillin-binding protein 1A/1B OS=Bacillus subtilis (strain 168) OX=224308 GN=ponA PE=1 SV=1
O87626	7.55e-66	33	606	36	720	Penicillin-binding protein 1A OS=Neisseria flavescens OX=484 GN=mrcA PE=3 SV=1
O87579	1.98e-65	56	607	64	722	Penicillin-binding protein 1A OS=Neisseria lactamica OX=486 GN=mrcA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.989240	0.009261	0.000222	0.000084	0.000039	0.001182

TMHMM  Annotations     

start	end
7	29