CAZyme Information: MGYG000004715_00058

Basic Information

• Species: Eubacterium_T saphenum_A
• Lineage: Bacteria; Firmicutes_A; Clostridia; Peptostreptococcales; Anaerovoracaceae; Eubacterium_T; Eubacterium_T saphenum_A
• CAZyme ID: MGYG000004715_00058
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
986	MGYG000004715_1|CGC3	105743.33	9.9183

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004715	2297538	MAG	China	Asia

• Gene Location: Start: 52820; End: 55780 Strand: +

Full Sequence      

MRSDKYNRAQGQPAGTASARKQKFLFPWQRKHKIIDTVLSVILLLIIAVMLYAAACIITA
PRIDVTPSNLKSTLSKTTVIYDAKGKKVDTVYSGENRIWLSYNEMPKNLVNAYVALEDKT
FWKHHGFNVTRIFGAIKESITSGGDISGTSTITQQLARNVYLKKSMSKRSMKRKIVEAWY
TMKLEHNLSKKQIMAAYLNTIYLGFNSNGVEAASEAYFGKSVSKLNTAQCALLASLPQWP
THYAPVQLISNEAVTTGATNILHRSSEGTYIMNDAGKTRRLTCLKLMRQQGYISQSEYEE
AAAIPLKELLHPRYSSGSTNNSYFTDYVVSQVISDLQKKDGLSYTDAHNKVYQGGLKIYS
TMDRKAQNVIEKEFKNDANFPAPKNISYDSSNNIINKYGSIIMYSYNNFFDASGNFTFPS
SEATKNSDGSITIMKGKRIRIYKTVSNGKTDYSVEFPTMYTTQNGSLYSIPGGYINIPQG
KKKLDSDGNLVVSASFVKSSAGRQMFKTTSDGSIMLTKNGYTLNQKVIQPQAAMTIVDNK
TGEIKAMVGGRKQTGKLLYNRAVKTRQPGSSIKPLAVYGAALQQTYEEHKAGKAHSYVNY
GIDTQGTAGWGNYITAGSEVIDEKTTVNGQVWPTNAEGGYSGVQTFRTALQKSINTCAVK
IFLQVGNKYSYNMVKKFGITSLVGSGSTNDRNAAALALGGMTNGVSTLEMANAYTTFANN
GVKSSKSICYTKVTDNGKKILSSASVKKKRVLDSGVAWIMRSCLQGVVSGGTGQKAAISG
VTVGGKTGTTSDEYDIWFDGFTPSYSASLWIGNDSNIQLSSKSDYAAALWSRIMSQIPNA
LKGSYPSMPSDVTQIGGEYYTTGTYGGAGGVSAPAGTGTDSSSQVQNGTQSSTGTNENGQ
QTENGQTTDNNLNGQDTQNQNNGTSGDNTTGGTDNTGGSQQNNSGTAGNNGSSATAGGNS
GSSSAASGGGTAKSSRQAAAGSGGAE

Enzyme Prediction     

No EC number prediction in MGYG000004715_00058.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	85	254	2.9e-56	0.9209039548022598

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR02074	PBP_1a_fam	5.18e-146	96	837	1	528	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG0744	MrcB	1.33e-138	38	895	24	654	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	6.89e-118	43	843	7	721	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
pfam00912	Transgly	9.84e-66	87	245	3	158	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.
TIGR02071	PBP_1b	1.15e-63	93	841	143	687	penicillin-binding protein 1B. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of a particular bifunctional transglycosylase/transpeptidase in E. coli and other Proteobacteria, designated penicillin-binding protein 1B. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ASS40664.1	0.0	3	865	30	893
ASS37973.1	5.07e-291	31	864	114	940
AVM47767.1	6.94e-291	31	864	114	940
AKT47092.1	1.91e-286	16	872	9	867
ASS37910.1	1.87e-261	38	869	211	1031

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7U4H_A	2.33e-99	65	833	8	756	ChainA, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX],7U4H_B Chain B, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX]
3UDF_A	2.65e-49	74	844	17	719	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
4OON_A	2.88e-42	80	834	23	719	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
3DWK_A	2.93e-39	80	848	10	609	ChainA, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_B Chain B, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_C Chain C, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_D Chain D, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL]
2OQO_A	9.50e-37	82	301	6	197	Crystalstructure of a peptidoglycan glycosyltransferase from a class A PBP: insight into bacterial cell wall synthesis [Aquifex aeolicus VF5],3D3H_A Crystal structure of a complex of the peptidoglycan glycosyltransferase domain from Aquifex aeolicus and neryl moenomycin A [Aquifex aeolicus],3NB7_A Crystal structure of Aquifex Aeolicus Peptidoglycan Glycosyltransferase in complex with Decarboxylated Neryl Moenomycin [Aquifex aeolicus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q97GR5	6.45e-87	59	864	57	693	Penicillin-binding protein 1A OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=pbpA PE=3 SV=1
A7FY32	2.81e-78	34	852	16	658	Penicillin-binding protein 1A OS=Clostridium botulinum (strain ATCC 19397 / Type A) OX=441770 GN=pbpA PE=3 SV=1
A5I6G4	2.81e-78	34	852	16	658	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) OX=441771 GN=pbpA PE=3 SV=1
A7GHV1	3.67e-78	34	852	16	658	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) OX=441772 GN=pbpA PE=3 SV=1
Q891X1	3.04e-76	37	854	23	675	Penicillin-binding protein 1A OS=Clostridium tetani (strain Massachusetts / E88) OX=212717 GN=pbpA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999899	0.000126	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
39	61