CAZyme Information: MGYG000004744_00348

Basic Information

• Species: Megamonas sp900556715
• Lineage: Bacteria; Firmicutes_C; Negativicutes; Selenomonadales; Selenomonadaceae; Megamonas; Megamonas sp900556715
• CAZyme ID: MGYG000004744_00348
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 1F

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
720		79184.39	6.1647

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004744	2057636	MAG	Denmark	Europe

• Gene Location: Start: 30284; End: 32446 Strand: -

Full Sequence      

MDKQPFNEKKSTSNHATDKKNKKKSFGFKKFFLALLTLFIVVFLGVGCGLVAIGLDGDKD
VSDIHPPASSQILDMNGNLITNIHATENRTLVTLDKIPANLQNAFIAVEDNRFYEHNGID
PRGIFRAIYSNLVSGEVSEGGSTITQQLAKNAFLSQDRTFKRKIQELFIALRLERQYTKE
EILEMYLNQIYFGRGAYGVQAAAQTYFGKDVSQLDLSECAMLAGIPRSPNYYSPLNNLSA
ANERKAEVLDQMVKYGYIDSATATKTKAESLRLVNPKEEEKNNTASYFIDYVTQEMIDKF
GADAVYKEGLKIYVSIDMNMQKAAEQSVNSLLPTYYSDANGLTQPQGALVAIDPKTGYIK
AMVGGRGTDQFNRAALAVRQPGSAFKPFTFATALENKMTPDTTIEDTPFKLGDWQPQNYD
RTFRGTVSMRQTAINSLNVPTIRLAEKLSIDKVLLTAQNLGISTLELNSKEANDKNLAAS
IGGLTNGVTVLDMAGAYSAFANHGVYTKPTAIVKVVDRKGKTIYEHKPETKQVLSERTAT
LLTDMLEDVITKGTGKAANINRPAAGKTGTTDNYQDAWFAGYTPDLVAVVWMGCDDNDVM
PGITGGTTPAKIWQNFMKTALASIPAKNFDGSDTSIDNPVQSVENNAKTTNKNVSHEKEK
PSKETPDKHKPVPNEQSNLNDAQQPIDENEPIEENIEQPSVPVPRQNLPEPSIQQGKGLN

Enzyme Prediction     

No EC number prediction in MGYG000004744_00348.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	77	253	2.4e-76	0.9887005649717514

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0744	MrcB	0.0	33	655	16	629	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	0.0	35	686	12	796	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	0.0	88	622	1	530	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
TIGR02071	PBP_1b	1.19e-135	70	618	115	680	penicillin-binding protein 1B. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of a particular bifunctional transglycosylase/transpeptidase in E. coli and other Proteobacteria, designated penicillin-binding protein 1B. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG4953	PbpC	2.06e-114	59	641	27	577	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BDA10313.1	0.0	1	720	1	720
QIB60439.1	0.0	1	720	1	720
CBL07106.1	0.0	19	659	5	645
SNU95661.1	0.0	1	720	1	724
BAL83676.1	2.75e-277	13	635	5	626

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4OON_A	1.58e-94	71	625	22	727	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
3UDF_A	7.82e-94	92	626	42	721	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
3ZG8_B	2.01e-85	173	598	3	436	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]
7U4H_A	1.56e-83	69	636	20	776	ChainA, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX],7U4H_B Chain B, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX]
3ZG7_B	1.33e-78	173	598	3	436	CrystalStructure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the apo form [Listeria monocytogenes]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P38050	5.74e-125	29	620	7	597	Penicillin-binding protein 1F OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpF PE=2 SV=2
O66874	9.82e-118	29	630	2	677	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1
P39793	1.32e-106	19	658	23	679	Penicillin-binding protein 1A/1B OS=Bacillus subtilis (strain 168) OX=224308 GN=ponA PE=1 SV=1
P40750	4.60e-105	55	620	41	623	Penicillin-binding protein 4 OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpD PE=1 SV=2
A5I6G4	8.54e-97	19	627	4	650	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) OX=441771 GN=pbpA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999917	0.000109	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
31	53