CAZyme Information: MGYG000001183_00039

Basic Information

• Species: UMGS1441 sp900551755
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; CAG-274; UMGS1441; UMGS1441 sp900551755
• CAZyme ID: MGYG000001183_00039
• CAZy Family: PL1
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1157	MGYG000001183_1|CGC1	122128.86	4.3385

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001183	2128086	MAG	Austria	Europe

• Gene Location: Start: 46850; End: 50323 Strand: +

Full Sequence      

MNNVLKRFLSVVLSAAMVFSSVAFVNISAVMADGSSYISEEGGWLESAYVEWTSVPNATG
FAAYVKKANDADSSYVKLDNELIRKYPTYFRADAVGLAAGQYVMKVVPYINGALDESAAF
VSGTLDVKANDRSGSAFSSKSPYYGKGVGAYNNDGTLKSDAQVVYVTKDTAKTVKADIIT
GKNGSSETITGFQSIIDAKQKGLDTRPLDFRIIGTIEKNDLDRISSSAEGLQIKGKNAGA
ELNITIEGIGEDAVIRGFGILCRNVGNVELRNFAVMLCMDDSISVDTDNNTLWIHNLDLF
YGSTGGDADQAKGDGTVDVKGKSNFVTVSYNHFWDSGKSSLCGMGSEVTSSHITYHHNWF
DHSDSRHPRIRVQSVHIYNNYFDGNAKYGVGVTLGSSAFVESNYFRNCKHPMMSSKQGSD
IMENDKTGVPDFSAKGTFSSENGGAIKAYNNHIEGSDADKFNGGVEPVYYDATATGTNGK
ATQYDAYLATTRDEKVPSTVKALAGGSSYNNFDTDTSNFDLGVDEANITPVEDVPSVVTK
YAGRVNGGDFKNDSKYTVLNDDKSYAVDTVLKAALSSYTSSLVSVGGLNAGGEVDTTVST
ETTTEVTTEKTSEVTTEKVSETTTEKATETTTSNSTPVEIGEAQTGFVSDNTSDTGAGVS
VTFNSATNKWTLSDSSTTAAAEIKIPFATQSKGKVIISGSVEPSTTASKWAFVQILGTKA
DGTQGEVFGFGGGANKTDLSARVNAGAYTKFGTFTAKNYDYTAIIDLDNKTVELTVDGVK
STYTGLDAESIEGVYSTTSKKGERNVSLSVPYVGVIGDAVETTTVAESSTETTTVEKTTV
NETSSETTTENVTPDPDFVYGDVDGSGERNIKDVNLLIEQIRNGAVNVIKGINDVNNDGV
VDTADVATLLQKVLNSNFKMPCEPNVTEPTTVTTIVATTEATTETTTVAPTEATTEDTTA
TTTTETTTVTTTVETTTEATTVEPTTVTTTETTTVTTTVETTTEATTAETTTEATTEGTT
SASASSWTAGDTVPSWLSVSGTVSGTAEKNSNGNVAFSTANGSAFANTIKLAENGTFTIT
PEKAGIVKVYVAANNNNANKGTLTATIDDKTVGTYNLPGRKDSSANAFEVTVDAANAGKA
ITFTTSYKALLFKVECN

Enzyme Prediction     

No EC number prediction in MGYG000001183_00039.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL1	241	407	4.1e-42	0.8118811881188119

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3866	PelB	6.96e-50	96	411	2	279	Pectate lyase [Carbohydrate transport and metabolism].
smart00656	Amb_all	3.84e-30	243	409	17	190	Amb_all domain.
pfam00544	Pec_lyase_C	1.39e-18	239	405	31	211	Pectate lyase. This enzyme forms a right handed beta helix structure. Pectate lyase is an enzyme involved in the maceration and soft rotting of plant tissue.
cd14256	Dockerin_I	2.69e-06	860	914	1	57	Type I dockerin repeat domain. Bacterial cohesin domains bind to a complementary protein domain named dockerin, and this interaction is required for the formation of the cellulosome, a cellulose-degrading complex. The cellulosome consists of scaffoldin, a noncatalytic scaffolding polypeptide, that comprises repeating cohesion modules and a single carbohydrate-binding module (CBM). Specific calcium-dependent interactions between cohesins and dockerins appear to be essential for cellulosome assembly. This subfamily represents type I dockerins, which are responsible for anchoring a variety of enzymatic domains to the complex.
pfam00404	Dockerin_1	0.005	861	914	1	56	Dockerin type I repeat. The dockerin repeat is the binding partner of the cohesin domain pfam00963. The cohesin-dockerin interaction is the crucial interaction for complex formation in the cellulosome. The dockerin repeats, each bearing homology to the EF-hand calcium-binding loop bind calcium.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCJ94010.1	3.45e-191	23	587	32	566
ACX62589.1	8.15e-189	1	600	1	573
AYB46946.1	1.90e-187	1	593	1	566
QOT09127.1	2.09e-186	1	593	1	566
ACR72247.1	2.11e-182	12	590	16	576

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3VMV_A	1.86e-24	181	407	20	248	Crystalstructure of pectate lyase Bsp165PelA from Bacillus sp. N165 [Bacillus sp. N16-5],3VMW_A Crystal structure of pectate lyase Bsp165PelA from Bacillus sp. N165 in complex with trigalacturonate [Bacillus sp. N16-5]
1VBL_A	4.84e-20	243	405	133	330	Structureof the thermostable pectate lyase PL 47 [Bacillus sp. TS-47]
1AIR_A	4.29e-19	212	433	63	290	ChainA, PECTATE LYASE C [Dickeya chrysanthemi],1O88_A Chain A, Pectate Lyase C [Dickeya chrysanthemi],1O8D_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8E_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8F_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8G_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8H_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8I_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8J_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8K_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8L_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8M_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1PLU_A Chain A, Protein (pectate Lyase C) [Dickeya chrysanthemi],2PEC_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi]
2EWE_A	1.04e-18	212	433	63	290	ChainA, Pectate lyase C [Dickeya chrysanthemi]
3ZSC_A	2.56e-14	245	390	71	221	Catalyticfunction and substrate recognition of the pectate lyase from Thermotoga maritima [Thermotoga maritima]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8GCB2	2.44e-26	181	414	57	281	Pectate trisaccharide-lyase OS=Bacillus licheniformis OX=1402 GN=pelA PE=1 SV=1
Q65DC2	2.44e-26	181	414	57	281	Pectate trisaccharide-lyase OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=BLi04129 PE=3 SV=1
B1B6T1	2.44e-26	181	414	57	281	Pectate trisaccharide-lyase OS=Bacillus sp. OX=1409 GN=pel PE=1 SV=1
Q5AVN4	1.86e-20	243	428	99	287	Pectate lyase A OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=plyA PE=1 SV=1
P0C1C1	1.21e-19	212	432	84	310	Pectate lyase 2 OS=Pectobacterium carotovorum OX=554 GN=pel2 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000329	0.999001	0.000158	0.000207	0.000159	0.000142

TMHMM  Annotations     

start	end
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