CAZyme Information: MGYG000000002_00658

Basic Information

• Species: Blautia_A faecis
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Blautia_A; Blautia_A faecis
• CAZyme ID: MGYG000000002_00658
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
653	MGYG000000002_2|CGC3	74439.08	4.9586

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000002	4433109	Isolate	United Kingdom	Europe

• Gene Location: Start: 228402; End: 230363 Strand: +

Full Sequence      

MLTDTTRSPYAKVHALDGMKVKWTGGLWKERFDTCANSTVPQLKHMFDSKDISHVVENFK
ICAGDAQGDFDGTVFGDGDFYKWMESAIYTAYQTDNQLLKDEIEKYIDLIGRAQQPDGYL
STKQIIGERSGNGVSRMGDINDFEVYNFGHMFTAACVHKRITGKDSFLAIAARTADYLEH
LYEEAERTGEVQTAVCPSHYMGLVEMYRTTGEERYLKLAKQAIALRDSVKNGLDDNQDKI
PLKQHEKILGHAVRANYLYAGVADLCLEEEDPDYIKVLHKVWRSLVDKKLYITGGCGALY
NGASPYGYFFDHQLVHQAYGYEYQLPNITAYNETCASLGGVFWAYRMFQLEPKAEYFDIL
ERMMLNTNLAALSLDGKRFFYENMLRRAQKLDYKLIWPLTRSEYILSYCCPPNLARTIAQ
SSEYAYLTSDNSVWTGMYGASEAQVKLENGGEFTLVQSTDYPFDGTIRFTAKDVKINAPV
HLRLRVPGWSAGGSIKGAGADRELTKEDAGTYLDILVEDPQNMDVQLSLDMKVRYTVANN
MVEETVGQAAIERGPLVYCCESVDTHAADLDDVYLDLNAEFTPVEFEIQGRRMMALETEE
YAIDRSEFDRNALYQPLKYHGMSRKHVRLIPYYAWDNRDYGEMRIWFPVAYTV

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	21	559	2.2e-160	0.9980916030534351

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07944	Glyco_hydro_127	1.99e-133	21	559	2	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG3533	COG3533	4.19e-109	25	646	20	584	Uncharacterized conserved protein, DUF1680 family [Function unknown].
cd04791	LanC_SerThrkinase	2.74e-05	160	304	100	249	Lanthionine synthetase C-like domain associated with serine/threonine kinases. Some members of this subgroup lack the zinc binding site and the active site residues, and therefore are most likely inactive. The function of this domain is unknown.
pfam05284	DUF736	7.31e-04	451	520	3	73	Protein of unknown function (DUF736). This family consists of several uncharacterized bacterial proteins of unknown function.
cd04792	LanM-like	0.008	160	231	606	688	Cyclases involved in the biosynthesis of class II lantibiotics, and similar proteins. LanM-like proteins. LanM is a bifunctional enzyme, involved in the synthesis of class II lantibiotics. It is responsible for both the dehydration and the cyclization of the precursor-peptide during lantibiotic synthesis. The C-terminal domain shows similarity to LanC, the cyclase component of the lan operon, but the N terminus seems to be unrelated to the dehydratase, LanB.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AGC67292.1	2.69e-301	1	651	1	651
AGI38357.1	2.69e-301	1	651	1	651
ANX00320.1	3.82e-301	1	651	1	651
ANW97794.1	1.79e-299	1	651	1	651
QOV18624.1	2.66e-286	1	649	1	649

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MQO_A	2.63e-159	2	650	25	697	Glycosidehydrolase BT_1003 [Bacteroides thetaiotaomicron]
4QJY_A	1.47e-100	28	646	23	644	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	1.03e-95	28	646	23	644	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
6EX6_A	1.02e-89	21	649	15	625	TheGH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482],6EX6_B The GH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482]
7EXU_A	4.77e-88	19	645	1	654	ChainA, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	5.53e-87	19	645	1	654	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000093	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000002_00658.