CAZyme Information: MGYG000000006_02513

Basic Information

• Species: Staphylococcus xylosus
• Lineage: Bacteria; Firmicutes; Bacilli; Staphylococcales; Staphylococcaceae; Staphylococcus; Staphylococcus xylosus
• CAZyme ID: MGYG000000006_02513
• CAZy Family: GH73
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1493		163199.21	4.3904

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000006	2822527	Isolate	United Kingdom	Europe

• Gene Location: Start: 18239; End: 22720 Strand: -

Full Sequence      

MAKNKNTYKVPSIIALTLAGTALTTHHAQAADKTQDQSPNKNFLNDDKALNQSEEIKSEI
SKPTANISGTQVYQDPTQIKQASTDLESNYDAQIDELNSQTSNQTPTQDTYNQNGEDQEA
QHDDNHNVETNQNTVESDVNSGETTNNDSTSDNTTEQDTDASQNNSAVTDKDDVTSDNKQ
DVAKDSDNSDTTSTVQDTDTSQEDSTDTSDKDVVTSDDKQDVTKDSNNSDTTSTVQDTDA
VQEDSTDTADKDDVTSDDKQDVTKDSNNSDTTSNIQDTDAVQEDSADDSDKDVITSDNKQ
DVAKDNDNSDTTSTVQDTDAVQDDSADDSDKDDVTSDDKQDVAKDNDNSNVTSNVQDTDA
VQEDSTDTADKDDVTSDDKQDVAKDNDNSNVTSNVQDTDAVQDDSTDTADKDDVTSDNKQ
DVAKDNDNSDTTSTVQDTDAVQEDSADDSDKDDVTSDDKQDVTKDNDNSDTTSTVQDTDA
VQEDSADTSEAANVAFYSAQATPKLRVARSTDSTVATRSAITQEATTRSTLPKYSPKVSS
SINNYIRKNNFKAPNYEQDIASYLPKYNYRYGKPEGIVMHDTANDNSTITGEINYMKNNY
QNAFVHAYVDGNRIIETANTDYLAWGGGAVANQRFIHVELVHTHDYDSFARSINNYADYA
ATNLQYYGLVPDSAEYDGAGTVWTHKAVSTYLGGTDHTDPHGYLAAHNYSYDELYDLIYE
KYLIKTGQAAAWGTSSSGSTSGNNSNNTGSSNTGVTAPTKTGSVKVTANNGVGRINTTND
GLYTTVYDQNGKKTDRTNQTLKVTKSATLGNENFYLVSDFNKGTLIGWVHQGDVNYNTAK
TATSINKTYQIKPGETIYTVPWGTSSQKAGTVSGKSTQTFKATKQQQIGKTNYIYGTVNN
LTGWVSLSKLSSTSNTATSPTTNSGKLTVNTLTNQQGTVAKNNHGVYTTVYDKQGVQKPY
VSGQTYKLSKKATLGNNAFYLITDNKTNTNIGWMQTGDITVKEVAKKATTNQTQSVNKIG
QLNTKNSGIKATVYDQQGKDATKLAGKTYNVTKQRTEGNNTYVLIQNVNQNTPIGWINTK
DINTRNLSQTNAKNGQYTVKATNNGLYAVPWGTKSQQLDKLNNLKNNQFNASKSVYVDKD
EYVYGIVNNKTGWIAANDLNKVQPSNNANVTNTQSVTKSAVTPYKYDYVIINRNGNYYLD
PTSTTASGSLKDFYESIFTVYETQVINGTTWYHGKLSNGKVVWIKETDLRKELVKYYDSG
ITLDQAVAIQKGLTAKPQIQHTPGKWEDATANEIKNAMDSSKLIKDETQKYQFLRLDKTQ
NIGATDLDKLLVGKGILEGQGAAFSEAAQTYDINEVYLISHALLETGNGTSKLANGGDVV
DGKVVTDGKNKYYNMFGIGAVDSDAVKQGFITAKNNGWNTVKKAIVGGAKFISESYIDKG
QNTLYKMRWNPENPGVHQYATDVAWASHNATRIKGFYDSMDKLGKYFDVDTYK

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH73	1342	1474	1.9e-23	0.9453125

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG4193	LytD	3.83e-74	1284	1493	46	245	Beta- N-acetylglucosaminidase [Carbohydrate transport and metabolism].
COG5632	CwlA	1.73e-51	539	875	2	302	N-acetylmuramoyl-L-alanine amidase CwlA [Cell wall/membrane/envelope biogenesis].
smart00047	LYZ2	2.02e-41	1329	1488	1	147	Lysozyme subfamily 2. Eubacterial enzymes distantly related to eukaryotic lysozymes.
smart00644	Ami_2	3.34e-27	572	700	1	126	Ami_2 domain.
cd06583	PGRP	6.00e-20	573	703	1	126	Peptidoglycan recognition proteins (PGRPs) are pattern recognition receptors that bind, and in certain cases, hydrolyze peptidoglycans (PGNs) of bacterial cell walls. PGRPs have been divided into three classes: short PGRPs (PGRP-S), that are small (20 kDa) extracellular proteins; intermediate PGRPs (PGRP-I) that are 40-45 kDa and are predicted to be transmembrane proteins; and long PGRPs (PGRP-L), up to 90 kDa, which may be either intracellular or transmembrane. Several structures of PGRPs are known in insects and mammals, some bound with substrates like Muramyl Tripeptide (MTP) or Tracheal Cytotoxin (TCT). The substrate binding site is conserved in PGRP-LCx, PGRP-LE, and PGRP-Ialpha proteins. This family includes Zn-dependent N-Acetylmuramoyl-L-alanine Amidase, EC:3.5.1.28. This enzyme cleaves the amide bond between N-acetylmuramoyl and L-amino acids, preferentially D-lactyl-L-Ala, in bacterial cell walls. The structure for the bacteriophage T7 lysozyme shows that two of the conserved histidines and a cysteine are zinc binding residues. Site-directed mutagenesis of T7 lysozyme indicates that two conserved residues, a Tyr and a Lys, are important for amidase activity.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AID43173.1	0.0	1	1493	1	1533
AID01012.1	0.0	1	1493	1	1515
ARD74055.1	0.0	1	1493	1	1514
QDW89566.1	0.0	1	1493	1	1613
CEF19268.1	0.0	1	1493	1	1494

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4KNK_A	4.75e-105	525	736	12	225	ChainA, Bifunctional autolysin [Staphylococcus aureus subsp. aureus NCTC 8325],4KNK_B Chain B, Bifunctional autolysin [Staphylococcus aureus subsp. aureus NCTC 8325],4KNL_A Chain A, Bifunctional autolysin [Staphylococcus aureus subsp. aureus NCTC 8325],4KNL_B Chain B, Bifunctional autolysin [Staphylococcus aureus subsp. aureus NCTC 8325],4KNL_C Chain C, Bifunctional autolysin [Staphylococcus aureus subsp. aureus NCTC 8325],4KNL_D Chain D, Bifunctional autolysin [Staphylococcus aureus subsp. aureus NCTC 8325]
3LAT_A	4.86e-102	529	733	7	213	Crystalstructure of Staphylococcus peptidoglycan hydrolase AmiE [Staphylococcus epidermidis],3LAT_B Crystal structure of Staphylococcus peptidoglycan hydrolase AmiE [Staphylococcus epidermidis]
6FXO_A	1.44e-97	1252	1493	3	244	ChainA, Bifunctional autolysin [Staphylococcus aureus subsp. aureus Mu50]
4EPC_A	4.60e-65	766	1155	24	328	Crystalstructure of Autolysin repeat domains from Staphylococcus epidermidis [Staphylococcus epidermidis]
6U0O_B	3.43e-58	1251	1493	40	276	ChainB, LYZ2 domain-containing protein [Staphylococcus aureus subsp. aureus NCTC 8325]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q5HQB9	0.0	529	1493	309	1335	Bifunctional autolysin OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) OX=176279 GN=atl PE=3 SV=1
Q6GI31	0.0	525	1493	206	1257	Bifunctional autolysin OS=Staphylococcus aureus (strain MRSA252) OX=282458 GN=atl PE=3 SV=1
Q6GAG0	0.0	525	1493	208	1250	Bifunctional autolysin OS=Staphylococcus aureus (strain MSSA476) OX=282459 GN=atl PE=3 SV=1
Q8CPQ1	0.0	529	1493	309	1335	Bifunctional autolysin OS=Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) OX=176280 GN=atl PE=3 SV=1
Q5HH31	0.0	525	1493	208	1256	Bifunctional autolysin OS=Staphylococcus aureus (strain COL) OX=93062 GN=atl PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000403	0.998699	0.000261	0.000226	0.000197	0.000169

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000006_02513.