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CAZyme Information: MGYG000000008_01867

You are here: Home > Sequence: MGYG000000008_01867

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species Lactobacillus johnsonii
Lineage Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lactobacillus; Lactobacillus johnsonii
CAZyme ID MGYG000000008_01867
CAZy Family GH68
CAZyme Description Inulosucrase
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
803 87787.04 4.7048
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000000008 1971706 Isolate United Kingdom Europe
Gene Location Start: 294;  End: 2705  Strand: +

Full Sequence      Download help

Enzyme Prediction      help

EC 2.4.1.10 2.4.1.9

CAZyme Signature Domains help

Family Start End Evalue family coverage
GH68 215 661 8.9e-144 0.9928057553956835

CDD Domains      download full data without filtering help

Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
cd08997 GH68 9.40e-121 270 651 1 354
Glycosyl hydrolase family 68, includes levansucrase, beta-fructofuranosidase and inulosucrase. Glycosyl hydrolase family 68 (GH68) consists of frucosyltransferases (FTFs) that include levansucrase (EC 2.4.1.10), beta-fructofuranosidase (EC 3.2.1.26) and inulosucrase (EC 2.4.1.9), all of which use sucrose as their preferential donor substrate. Levansucrase, also known as beta-D-fructofuranosyl transferase, catalyzes the transfer of the sucrose fructosyl moiety to a growing levan chain. Similarly, inulosucrase catalyzes long inulin-type of fructans, and beta-fructofuranosidases create fructooligosaccharides (FOS). However, in the absence of high fructan/sucrose ratio, some GH68 enzymes can also use fructan as donor substrate. GH68 retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. Biotechnological applications of these enzymes include use of inulin in inexpensive production of rich fructose syrups as well as use of FOS as health-promoting pre-biotics.
pfam02435 Glyco_hydro_68 5.78e-120 217 653 3 411
Levansucrase/Invertase. This Pfam family consists of the glycosyl hydrolase 68 family, including several bacterial levansucrase enzymes, and invertase from zymomonas.
cd08979 GH_J 8.12e-19 271 641 1 291
Glycosyl hydrolase families 32 and 68, which form the clan GH-J. This glycosyl hydrolase family clan J (according to carbohydrate-active enzymes database (CAZY)) includes family 32 (GH32) and 68 (GH68). GH32 enzymes include invertase (EC 3.2.1.26) and other other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). The GH68 family consists of frucosyltransferases (FTFs) that include levansucrase (EC 2.4.1.10, also known as beta-D-fructofuranosyl transferase), beta-fructofuranosidase (EC 3.2.1.26) and inulosucrase (EC 2.4.1.9). GH32 and GH68 family enzymes are retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) and catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
PRK08581 PRK08581 1.41e-06 5 269 3 283
amidase domain-containing protein.
TIGR01167 LPXTG_anchor 0.004 766 802 1 34
LPXTG-motif cell wall anchor domain. This model describes the LPXTG motif-containing region found at the C-terminus of many surface proteins of Streptococcus and Streptomyces species. Cleavage between the Thr and Gly by sortase or a related enzyme leads to covalent anchoring at the new C-terminal Thr to the cell wall. Hits that do not lie at the C-terminus or are not found in Gram-positive bacteria are probably false-positive. A common feature of this proteins containing this domain appears to be a high proportion of charged and zwitterionic residues immediatedly upstream of the LPXTG motif. This model differs from other descriptions of the LPXTG region by including a portion of that upstream charged region. [Cell envelope, Other]

CAZyme Hits      help

Hit ID E-Value Query Start Query End Hit Start Hit End
AYN50318.1 0.0 1 803 1 803
QIA87843.1 0.0 1 803 1 803
AHA97605.1 0.0 1 803 1 803
AAS08734.1 0.0 1 803 1 797
QGY96906.1 0.0 1 803 1 788

PDB Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
2YFR_A 0.0 145 708 2 565
Crystalstructure of inulosucrase from Lactobacillus johnsonii NCC533 [Lactobacillus johnsonii],2YFT_A Crystal structure of inulosucrase from Lactobacillus johnsonii NCC533 in complex with 1-kestose [Lactobacillus johnsonii]
2YFS_A 0.0 145 708 2 565
Crystalstructure of inulosucrase from Lactobacillus johnsonii NCC533 in complex with sucrose [Lactobacillus johnsonii]
3OM6_A 1.66e-86 218 659 16 449
ChainA, Levansucrase [Priestia megaterium],3OM6_B Chain B, Levansucrase [Priestia megaterium],3OM6_C Chain C, Levansucrase [Priestia megaterium],3OM6_D Chain D, Levansucrase [Priestia megaterium]
3OM7_A 2.32e-86 218 659 16 449
ChainA, Levansucrase [Priestia megaterium],3OM7_B Chain B, Levansucrase [Priestia megaterium],3OM7_C Chain C, Levansucrase [Priestia megaterium],3OM7_D Chain D, Levansucrase [Priestia megaterium]
3OM4_A 4.53e-86 218 659 16 449
ChainA, Levansucrase [Priestia megaterium],3OM4_B Chain B, Levansucrase [Priestia megaterium],3OM4_C Chain C, Levansucrase [Priestia megaterium],3OM4_D Chain D, Levansucrase [Priestia megaterium]

Swiss-Prot Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
D3WYV9 0.0 1 711 1 705
Inulosucrase OS=Lactobacillus gasseri OX=1596 GN=inuGB PE=1 SV=1
Q74K42 0.0 1 803 1 797
Inulosucrase OS=Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533) OX=257314 GN=inuJ PE=1 SV=1
D3WYW0 3.14e-288 1 803 1 768
Levansucrase OS=Lactobacillus gasseri OX=1596 GN=levG PE=1 SV=1
P11701 1.41e-244 179 717 153 687
Levansucrase OS=Streptococcus mutans serotype c (strain ATCC 700610 / UA159) OX=210007 GN=ftf PE=3 SV=2
Q70XJ9 3.69e-228 187 712 227 756
Levansucrase OS=Fructilactobacillus sanfranciscensis OX=1625 GN=levS PE=1 SV=1

SignalP and Lipop Annotations help

This protein is predicted as SP

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
0.000510 0.998658 0.000261 0.000213 0.000183 0.000153

TMHMM  Annotations      download full data without filtering help

start end
775 797