CAZyme Information: MGYG000000009_01456

Basic Information

• Species: Ligilactobacillus murinus
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Ligilactobacillus; Ligilactobacillus murinus
• CAZyme ID: MGYG000000009_01456
• CAZy Family: GH25
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1791	MGYG000000009_33|CGC1	201375.43	4.6246

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000009	2219792	Isolate	United Kingdom	Europe

• Gene Location: Start: 2704; End: 8079 Strand: -

Full Sequence      

MEYRRKKRVLASYRKMNKRKLWLASGMTMMAISTGMYATQNVKADQVSVEAQNTQQVATD
KDNSNVSNEQNDQTTVQLDQNISEREAVQNENFNTNDQAKVELNATAQEDNSNTTEQVQT
KDTSLVDDAIEDANATQELQLTTDEKKVNENAGEKVQAQQLQVNKVATKTQTPDAIYIGD
SQYTHGDAIDVASYQSWLNQDDFNKLKQLGIKAVVVKLTEGQSYVNPAATKQINYAKNAG
LTVSVYHYARFNNATTGYAEGQHIANTMENLGLGQDTLIFADMEDHSTYSNDVRGNLNSF
WNALTAAGFNNHGVYTGGGYPYHEAASETVGKAKTWWSQYPFVPTNNGYYEKEWKNLGYG
AWQFSSTAYIPGRENMGALDLSIDFNGLFSKSDTTEKTVTQQVFKDGHWYLIDQKTGQYL
TGIQPITDQNKVVYYNDAGQMQYGQQNINGYWYNFGTFDGAMKTGLVRIPEQNKTVYYAD
NGRMQYGWQWVNNATRYFDTFNGAMVTGQQRINGHWYLFDNNGAMQRGFQNLKAYGDDKT
VYYNQDGWMLYGWQWVDNATRYFDTFNGAMATGQKFITGHWYLFDNNGAMQRGFQNLKDY
GDNKTVYYNQDGWMQYGWQWLDNATRYFDTFDGAMAMGQKNIANHWYLFDKAGAMQRGFQ
NLKDYGENKTAYYNQDGWMQYGWQWLDNATHYFDTYDGAMAIGQKKIDDHWYLFDKNGAM
QRGFQNLKDYGDNKTVYYNQDGWMQYGWQWLDNATRYFDTYDGAMAIGQKKINDHWYLFD
KNGAMQRGFQYLPEDKKVAYYNQDGWMLYGKQDISGKTYSFNTYNGALENMDGQQKLGDH
WYLFNKGEVLTGFQEIKDQNKTVYYDPSTARMQYGWQTIANNKYYFDTYDGAMYTGSHLI
DGVDYSFDDDGTLLPLTIQAYREMVAKEVADDIKKNYAQSIDYDWNNQNDNYLAFTLHDT
AQLVARNNLAATSEAVQKNLVNNSLFTGTANIVWTKSYKDMHQAITQAANDFMTTFVPTI
DLSKSAIGVGAKEMDDSINVAIIIFTPGKDDKPKEELADSALTANVSEVYTAPGVNTQAH
DGLRSGDTISASELGNTFKVSPSLLNGVKGQKIGEQNLKLIFSSLPGNETGLEGQKLYKG
IDGNDYHYVFWLNDTFAGQKVGKQEAFLAANKDAVYGDPLKVSYSATLTWGAPVEVSIGV
TGVPTSQMTDEQIDLAYKTGTNTGLRYDAVTVKPIEGMTEDMIRGVDIGSYQSLINAGVK
FYDFDGNEAPIYKVLKDAGVNWIRLRVWNDPYDVNNNTYAGGDCSEANVVQMAKDASKYG
LKVLLDFHYSDFWADPAKQPLPKAWEDQTGDSLAKSVYNYTSKVLTDLKNAGVDVGMVQV
GNEITNGVFGIYSNRDRGESYLNIWGNKEKAAQISRYLNAGSKAVRDVLPDAKVAIQLET
PNVPKYTNIMNALRDNNVDYDILGTSYYPFWSTHDGNGWYDDVDMGWGANTPKSLEGIEK
LAKQEYGKQVVVLETGWINNVRDSDGTGNSIGPDSEIQAYSHDPQGQVDAMADMYKALVA
QGGLGGFWWEPAWIPVKVGWENWQFNKDASDKYGTGWASQYAVGYAPDSVMYYNGQPTWG
GSTWDNVALFDDLGHPLQSLKMYNGFLHGYESPKAPEKATSTLNFKVNKVWNSNNVALKD
GIVEGSTLTASDMLEPLSAAANELLSGTGAFTQENLEKIASGLGGMTTALEGSKTYYADN
GDAYHYVFWFTEGKTAEQKAWNFYNDNMNAKYGEPLVVNVEATVTWGAAKA

Enzyme Prediction     

EC	3.2.1.89

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH53	1243	1642	7.5e-110	0.9941520467836257
GH25	189	372	3.1e-32	0.9887005649717514

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3867	GanB	6.96e-100	1219	1655	16	403	Arabinogalactan endo-1,4-beta-galactosidase [Carbohydrate transport and metabolism].
pfam07745	Glyco_hydro_53	1.89e-90	1243	1641	1	331	Glycosyl hydrolase family 53. This domain belongs to family 53 of the glycosyl hydrolase classification. These enzymes are enzymes are endo-1,4- beta-galactanases (EC:3.2.1.89). The structure of this domain is known and has a TIM barrel fold.
cd06522	GH25_AtlA-like	3.84e-61	187	385	2	192	AtlA is an autolysin found in Gram-positive lactic acid bacteria that degrades bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues. This family includes the AtlA and Aml autolysins from Streptococcus mutans which have a C-terminal glycosyl hydrolase family 25 (GH25) catalytic domain as well as six tandem N-terminal repeats of the GBS (group B Streptococcus) Bsp-like peptidoglycan-binding domain. Other members of this family have one or more C-terminal peptidoglycan-binding domain(s) (SH3 or LysM) in addition to the GH25 domain.
pfam01183	Glyco_hydro_25	6.28e-26	189	372	1	179	Glycosyl hydrolases family 25.
NF033930	pneumo_PspA	5.94e-23	449	720	449	642	pneumococcal surface protein A. The pneumococcal surface protein proteins, found in Streptococcus pneumoniae, are repetitive, with patterns of localized high sequence identity across pairs of proteins given different specific names that recombination may be presumed. This protein, PspA, has an N-terminal region that lacks a cross-wall-targeting YSIRK type extended signal peptide, in contrast to the closely related choline-binding protein CbpA which has a similar C-terminus but a YSIRK-containing region at the N-terminus.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QIA89409.1	0.0	394	1409	125	1097
AXX74880.1	5.69e-295	899	1784	527	1387
AEV96111.1	1.08e-293	768	1790	679	1720
QYH53989.1	2.79e-287	639	1790	190	1471
QHM44962.1	3.82e-286	700	1790	626	1815

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1R8L_A	3.17e-87	1222	1643	4	388	Thestructure of endo-beta-1,4-galactanase from Bacillus licheniformis [Bacillus licheniformis],1R8L_B The structure of endo-beta-1,4-galactanase from Bacillus licheniformis [Bacillus licheniformis],1UR0_A The structure of endo-beta-1,4-galactanase from Bacillus licheniformis in complex with two oligosaccharide products. [Bacillus licheniformis],1UR0_B The structure of endo-beta-1,4-galactanase from Bacillus licheniformis in complex with two oligosaccharide products. [Bacillus licheniformis],1UR4_A The structure of endo-beta-1,4-galactanase from Bacillus licheniformis in complex with two oligosaccharide products. [Bacillus licheniformis],1UR4_B The structure of endo-beta-1,4-galactanase from Bacillus licheniformis in complex with two oligosaccharide products. [Bacillus licheniformis],2CCR_A Structure of Beta-1,4-Galactanase [Bacillus licheniformis],2CCR_B Structure of Beta-1,4-Galactanase [Bacillus licheniformis],2J74_A Structure of Beta-1,4-Galactanase [Bacillus licheniformis],2J74_B Structure of Beta-1,4-Galactanase [Bacillus licheniformis]
2GFT_A	2.01e-86	1222	1643	4	388	ChainA, Glycosyl Hydrolase Family 53 [Bacillus licheniformis],2GFT_B Chain B, Glycosyl Hydrolase Family 53 [Bacillus licheniformis]
7OSK_A	1.60e-65	1228	1643	36	391	ChainA, Arabinogalactan endo-1,4-beta-galactosidase [Ignisphaera aggregans DSM 17230],7OSK_B Chain B, Arabinogalactan endo-1,4-beta-galactosidase [Ignisphaera aggregans DSM 17230]
6GPA_A	1.05e-38	1240	1571	5	286	Beta-1,4-galactanasefrom Bacteroides thetaiotaomicron with galactose [Bacteroides thetaiotaomicron VPI-5482],6GPA_B Beta-1,4-galactanase from Bacteroides thetaiotaomicron with galactose [Bacteroides thetaiotaomicron VPI-5482]
6GP5_A	2.65e-38	1240	1571	41	322	Beta-1,4-galactanasefrom Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],6GP5_B Beta-1,4-galactanase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O07013	7.94e-88	1225	1643	36	417	Endo-beta-1,4-galactanase OS=Bacillus subtilis (strain 168) OX=224308 GN=ganB PE=1 SV=1
Q65CX5	3.72e-86	1222	1643	29	413	Endo-beta-1,4-galactanase OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=ganB PE=1 SV=1
P48843	4.07e-41	1240	1639	4	342	Uncharacterized protein in bgaB 5'region (Fragment) OS=Niallia circulans OX=1397 PE=3 SV=1
P83692	1.40e-36	1244	1574	5	301	Arabinogalactan endo-beta-1,4-galactanase OS=Thermothelomyces thermophilus OX=78579 PE=1 SV=1
A1D3T4	5.08e-35	1244	1574	26	324	Probable arabinogalactan endo-beta-1,4-galactanase A OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=galA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.056645	0.928690	0.011014	0.002804	0.000509	0.000312

TMHMM  Annotations     

start	end
21	38