CAZyme Information: MGYG000000011_00417

Basic Information

• Species: Staphylococcus xylosus_B
• Lineage: Bacteria; Firmicutes; Bacilli; Staphylococcales; Staphylococcaceae; Staphylococcus; Staphylococcus xylosus_B
• CAZyme ID: MGYG000000011_00417
• CAZy Family: GH73
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1525		166536.22	4.5937

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000011	2927352	Isolate	United Kingdom	Europe

• Gene Location: Start: 417341; End: 421918 Strand: -

Full Sequence      

MAKNKNSYKVPSIIALTLAGTALTTHHAQAADKTQDQTPNKNILNDDKALNQSEKIKSEI
SKPTANISGTQVYQDPTQIKQSSTDHDSNYDAQIDELNNQISDQTPTQDTYNQNGQGQEA
QQNDNHNIETNQDAVQSNVTSNETTNNDNGSDNTTEQDTDAVQKDSAGTDKDEVISEDKQ
VVTNNSVVSDDSSTKEDTDAIQNDNASTDKDEVTSEDKQDMTNDSVNSDDSSTKENTDAV
QNDSVGTDKDEVTSEDNQDVTNDSVNSDDSSTKENTDAVQNDSAGTDKEEIISEDNQNVT
NDSVNSDDSSTKENTDAVQKNSAGTDKDEVTSEDKQVVTNHSVNSDDSSTKEDTDAIQND
NASTDKDEVTSEDNQDVTNHSVNAVDSSTKEDTDAVQNDSAGTDKDEVTSEDNQDVTNHS
VNSDDSSTKEDTDAVQNDSAGTDKDEVTSEDKQVVTNHSVNSDDSSTKEDTDAIQNDSTG
TDKDEVTSKDKQVVTNHSVNSDDSSTKEDTDAVQNDSTDTTKVANANLYNTQTTPKLRVA
RSANSTVATRSTLTKEATTRSTLPKYSPKVSSSINNYIRKNNFKAPNYEQDIASYLPKYN
YRYGKPEGIVMHDTANDNSTITGEINYMKNNYQNAFVHAYVDGNRIIETANTDYLAWGGG
AVANQRFIHVELVHTHDYDSFARSINNYADYAATNLQYYGLAPDSAEYDGVGTVWTHKAV
SNYLGGTDHTDPHGYLAAHNYSYDELYDLIYEKYLIKTGQAAAWGTSTSGSTSGNSSNNT
GSSNTGATTPAKTGSLKVTANNGVGRINTTNDGLYTTVYDQNGKKTDRTNQTLKITKSAT
LGNENFYLVSDFNKGTLIGWVHQGDVNYNTAKAATSINKTYQIKSGETIYTVPWGTGSQK
AGTVSGKSTQTFKATKQQQIGNTNYIYGTVNNLSGWVSLSKLSSTSNTATSPTTNSGKLT
VDILTNQQGTVAKNNHGVYTTVYDKQGVQKPYVNGQTYKLSKKATLGNNSFYLITDNKTN
TNIGWMQTGDITVKEVTKKANTNQTQSVSKIGQLNTKNSGIKATVYDQQGKDATKLAGKT
YNVTKQRTEGNNTYVLIQNVNQNTPIGWINTKDINTRNLSQTSAKNGQYTVKATNNGLYA
VPWGTKSQQLDTLNNLKNNHFNASKSVYVDKDEYVYGIVNNKTGWIAANDLNKVQPSNNA
NVTNSQSVTKSAVTPYKYDYVVFNKNGNYYLDPTSTTSAGSLKDFYETIFTVYETQVING
TTWYHGKLSNGKIVWIKEADLRKELVKYYDSGITLDQAVAIQKGLTAKPQIQHTPGKWED
ATASEIKNAMDSSKLIKNETQKYQFLRLDKTQNIGAADLDKLLVGKGILEGQGAAFSEAA
QTYDINEVYLISHALLETGNGTSKLANGGDVVDNKVVTDGPNKYYNMFGIGAVDSDAVKQ
GFITAKNNGWNTVKKAIVGGAKFISESYIDKGQNTLYKMRWNPENPGVHQYATDVAWASH
NATRIKGFYDSMDKLGKYFDVDTYK

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH73	1374	1506	1.6e-23	0.9453125

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG4193	LytD	1.83e-72	1316	1525	46	245	Beta- N-acetylglucosaminidase [Carbohydrate transport and metabolism].
COG5632	CwlA	4.43e-50	571	907	2	302	N-acetylmuramoyl-L-alanine amidase CwlA [Cell wall/membrane/envelope biogenesis].
smart00047	LYZ2	3.22e-41	1361	1520	1	147	Lysozyme subfamily 2. Eubacterial enzymes distantly related to eukaryotic lysozymes.
smart00644	Ami_2	1.04e-26	604	732	1	126	Ami_2 domain.
cd06583	PGRP	2.54e-20	605	735	1	126	Peptidoglycan recognition proteins (PGRPs) are pattern recognition receptors that bind, and in certain cases, hydrolyze peptidoglycans (PGNs) of bacterial cell walls. PGRPs have been divided into three classes: short PGRPs (PGRP-S), that are small (20 kDa) extracellular proteins; intermediate PGRPs (PGRP-I) that are 40-45 kDa and are predicted to be transmembrane proteins; and long PGRPs (PGRP-L), up to 90 kDa, which may be either intracellular or transmembrane. Several structures of PGRPs are known in insects and mammals, some bound with substrates like Muramyl Tripeptide (MTP) or Tracheal Cytotoxin (TCT). The substrate binding site is conserved in PGRP-LCx, PGRP-LE, and PGRP-Ialpha proteins. This family includes Zn-dependent N-Acetylmuramoyl-L-alanine Amidase, EC:3.5.1.28. This enzyme cleaves the amide bond between N-acetylmuramoyl and L-amino acids, preferentially D-lactyl-L-Ala, in bacterial cell walls. The structure for the bacteriophage T7 lysozyme shows that two of the conserved histidines and a cysteine are zinc binding residues. Site-directed mutagenesis of T7 lysozyme indicates that two conserved residues, a Tyr and a Lys, are important for amidase activity.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AID01012.1	0.0	1	1525	1	1515
CEF19268.1	0.0	1	1525	1	1494
QDW89566.1	0.0	1	1525	1	1613
AID43173.1	0.0	1	1525	1	1533
CAC51118.1	0.0	1	1525	1	1564

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4KNK_A	1.40e-103	557	768	12	225	ChainA, Bifunctional autolysin [Staphylococcus aureus subsp. aureus NCTC 8325],4KNK_B Chain B, Bifunctional autolysin [Staphylococcus aureus subsp. aureus NCTC 8325],4KNL_A Chain A, Bifunctional autolysin [Staphylococcus aureus subsp. aureus NCTC 8325],4KNL_B Chain B, Bifunctional autolysin [Staphylococcus aureus subsp. aureus NCTC 8325],4KNL_C Chain C, Bifunctional autolysin [Staphylococcus aureus subsp. aureus NCTC 8325],4KNL_D Chain D, Bifunctional autolysin [Staphylococcus aureus subsp. aureus NCTC 8325]
3LAT_A	7.95e-102	561	765	7	213	Crystalstructure of Staphylococcus peptidoglycan hydrolase AmiE [Staphylococcus epidermidis],3LAT_B Crystal structure of Staphylococcus peptidoglycan hydrolase AmiE [Staphylococcus epidermidis]
6FXO_A	8.08e-97	1284	1525	3	244	ChainA, Bifunctional autolysin [Staphylococcus aureus subsp. aureus Mu50]
4EPC_A	2.55e-65	796	1187	22	328	Crystalstructure of Autolysin repeat domains from Staphylococcus epidermidis [Staphylococcus epidermidis]
6U0O_B	3.52e-58	1283	1525	40	276	ChainB, LYZ2 domain-containing protein [Staphylococcus aureus subsp. aureus NCTC 8325]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q5HQB9	0.0	561	1525	309	1335	Bifunctional autolysin OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) OX=176279 GN=atl PE=3 SV=1
Q6GI31	0.0	557	1525	206	1257	Bifunctional autolysin OS=Staphylococcus aureus (strain MRSA252) OX=282458 GN=atl PE=3 SV=1
Q6GAG0	0.0	557	1525	208	1250	Bifunctional autolysin OS=Staphylococcus aureus (strain MSSA476) OX=282459 GN=atl PE=3 SV=1
Q8CPQ1	0.0	561	1525	309	1335	Bifunctional autolysin OS=Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) OX=176280 GN=atl PE=3 SV=1
Q5HH31	0.0	557	1525	208	1256	Bifunctional autolysin OS=Staphylococcus aureus (strain COL) OX=93062 GN=atl PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000318	0.998889	0.000228	0.000207	0.000180	0.000155

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000011_00417.