CAZyme Information: MGYG000000013_00524

Basic Information

• Species: Bacteroides sp902362375
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides sp902362375
• CAZyme ID: MGYG000000013_00524
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
987	MGYG000000013_2|CGC3	112916.08	7.1653

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000013	6368149	Isolate	United Kingdom	Europe

• Gene Location: Start: 54533; End: 57496 Strand: -

Full Sequence      

MKHQLYLFVFLLITLSIINSQAAERKKYNFNSEWKLQIGEFPKAKDTKFDDSEWKQVTLP
HAFNEDEAFKLSIEQLTDTVVWYRKSFRIPELKSNQKVFIEFEGVRQRGDFYLNGHNLGR
HENGVMAVGFDLTPYMKEGENVIAVRTDNNWMYREEGTNSKFQWNDRNFNANYGGIPKNV
FLYVTDNVYQTLPLYSNLKTTGVYVYAKDFDIKGHKATIHAESEVRNDSKSPRQFSYQVT
VLDADGKRLKTFQGDKVTLKAGETKTVKASAIINNLHFWSWGYGYLYTVKTALKDDSNQI
FDEVSTRTGFRKTRFVEGKIELNDRVIQMKGYAQRTSNEWPAVGLSVPAWLSDFSNGLMV
KGNANLVRWMHVTPWKQDVESCDRVGLIQAMPAGDAEKDREGRQWEQRVELMRDAIIYNR
NNPSILFYECGNKAISREHMIEMKAVRDKYDPFGGRAIGSREMLDIREAEYGGEMLYINR
SEHHPMWATEYCRDEGLRKYWDEYSYPFHKEGDGPLYKGKPATDYNRNQDEFAITMIARW
YDYWRERPGTGNRVSSGGTKIIFSDTNTHYRGAENYRRSGVTDAMRIEKDAFYAHQVMWN
GWVDTEEDQTYIIGHWNYPENTVKPIHVVSTGEEVELFLNGKSLGKGKRQYNFLFTFDNI
TFKPGKLEAVSYNQAGKEISRYAIHTAGEPAKLKLTAIQNPEGFHGDGADMALIQVEVVD
KDGKRCPLDNRTVQFTLNGQAEWRGGIAQGENNHILDTKLPVECGINRALIRSTTTAGKV
TLTAQAKGLLAATLTLETVPVKVTEGLSTYLPQETLKGQLDRGETPSTPSYQDSKKGVRI
VSAKAGSNNSDAEKSYDDIELTEWKNDGKLSTAWITYTLEREAEIDDICIKLQGWRSRSY
PLEVYAGNILIWNGNTDKSLGYIHLNVEKPVRANTITIRLKGNTSDKDAFGQIIEVEAKA
ANKMEQEKSSSKNQLRIIEVEFLETIK

Enzyme Prediction     

EC	3.2.1.31

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	19	772	3.6e-68	0.8204787234042553

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	8.17e-30	24	513	9	495	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	1.25e-23	81	453	67	432	beta-D-glucuronidase; Provisional
PRK10340	ebgA	8.56e-21	1	452	15	468	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam02837	Glyco_hydro_2_N	5.00e-18	31	185	5	168	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.
pfam18565	Glyco_hydro2_C5	1.27e-17	693	795	1	103	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNL41159.1	0.0	1	987	1	987
ALJ49030.1	0.0	1	987	1	987
QRQ55837.1	0.0	1	987	1	987
SCV08447.1	0.0	1	987	1	987
QUT27395.1	0.0	1	987	1	987

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6HPD_A	2.80e-154	22	813	32	819	Thestructure of a beta-glucuronidase from glycoside hydrolase family 2 [Formosa agariphila KMM 3901]
7CWD_A	1.45e-63	26	798	2	801	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
4YPJ_A	4.93e-57	26	789	8	798	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
5T98_A	4.53e-56	29	796	29	818	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]
4CUC_A	7.70e-48	20	786	8	832	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA. [Streptococcus pneumoniae TIGR4]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KN75	1.22e-153	22	813	23	810	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1
T2KM09	1.34e-47	11	742	32	761	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
A7LXS9	4.26e-46	19	794	36	844	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KPJ7	6.91e-38	25	726	51	757	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P77989	1.02e-36	25	786	3	725	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000207	0.999172	0.000156	0.000158	0.000140	0.000135

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000013_00524.