CAZyme Information: MGYG000000019_02753

Basic Information

• Species: Enterobacter cloacae_O
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Enterobacter; Enterobacter cloacae_O
• CAZyme ID: MGYG000000019_02753
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 1C

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
754	MGYG000000019_11|CGC2	83347.79	9.7928

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000019	4947669	Isolate	United Kingdom	Europe

• Gene Location: Start: 66340; End: 68604 Strand: +

Full Sequence      

MALIVAADRLWPLPLKEVNPSRVVVDEQGVPLWRFADSGGIWRYPVTIEEVSPRYLEALI
QYEDRWFWEHPGVNPLSVLRAAWQDLTSGRVVSGGSTLTMQVARLLDPHPRTFGGKIRQL
WRAMQLEWHLSKREILTLYLNRAPFGGTLQGVGAASWTYLGKPPSRLSYSDAALLAVLPQ
APSRLRPDRWPERAEAARNKVLDRMMAQGVWSEKQVKESREEPVWLAPRQMPQLAPLFSR
MMLGKSRETKIATTLDATLQRQLEELAMNWKSRLPARSSLAMIVVDHTDMKVRGWVGSVD
INDDSRFSHVDMVNAIRSPGSVLKPFVYGMALDDGLIHPASLLQDVPRKTGDYRPGNFDS
GFHGPVSMSEALVRSLNLPAVQVLEAYGPKRFAGMLGNAGLPLILPAGAQPNLSLILGGA
GARLADIAAAYSAFARQGKAGRLRLQPGDPLVERPLLSQGAAWIIRRILAGEAQPLPDSA
LPQVAPLAWKTGTSYGYRDAWAIGLNARYVIGIWTGRPDGTPVAGQFGFASAVPLLNQVN
NLLQSRSTVDEARLPRDPRPASVGRGVICWPGGQSLPEGSENCRRRLSTWLLEGSEPPTL
LLPEQEGIRGIRFPVWLNKDGQRVAADCPDATEKVLDVWPLPLEPWLPVAERRAARLPAR
STVCPPLDNHNSAPLMLSGVREGAVIKRLPGEKNMTLFVSTSGGEGQRWWFLNGEPLEGR
QRNYSLQLYLPGNYQLIVMDETGQTATVNFELDR

Enzyme Prediction     

No EC number prediction in MGYG000000019_02753.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	42	205	5.4e-52	0.9265536723163842

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG4953	PbpC	0.0	3	754	17	733	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].
TIGR02073	PBP_1c	0.0	12	751	1	725	penicillin-binding protein 1C. This subfamily of the penicillin binding proteins includes the member from E. coli designated penicillin-binding protein 1C. Members have both transglycosylase and transpeptidase domains and are involved in forming cross-links in the late stages of peptidoglycan biosynthesis. All members of this subfamily are presumed to have the same basic function. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
PRK11240	PRK11240	0.0	2	753	21	772	penicillin-binding protein 1C; Provisional
COG0744	MrcB	4.95e-114	38	536	71	589	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	1.58e-112	43	516	2	500	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QLA69664.1	0.0	1	754	22	775
QKZ98201.1	0.0	1	754	22	775
AOQ00890.1	0.0	3	754	24	775
QJP77775.1	0.0	3	754	24	775
QMI69635.1	0.0	3	754	24	775

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4OON_A	1.20e-41	36	535	32	714	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
3UDF_A	2.99e-34	36	447	32	544	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
2OQO_A	3.14e-34	43	219	20	197	Crystalstructure of a peptidoglycan glycosyltransferase from a class A PBP: insight into bacterial cell wall synthesis [Aquifex aeolicus VF5],3D3H_A Crystal structure of a complex of the peptidoglycan glycosyltransferase domain from Aquifex aeolicus and neryl moenomycin A [Aquifex aeolicus],3NB7_A Crystal structure of Aquifex Aeolicus Peptidoglycan Glycosyltransferase in complex with Decarboxylated Neryl Moenomycin [Aquifex aeolicus]
3NB6_A	1.09e-33	43	219	20	197	Crystalstructure of Aquifex aeolicus peptidoglycan glycosyltransferase in complex with Methylphosphoryl Neryl Moenomycin [Aquifex aeolicus]
5FGZ_A	1.26e-31	56	522	169	673	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P76577	0.0	7	750	28	767	Penicillin-binding protein 1C OS=Escherichia coli (strain K12) OX=83333 GN=pbpC PE=1 SV=1
P38050	2.21e-47	18	516	45	569	Penicillin-binding protein 1F OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpF PE=2 SV=2
Q9KUC0	2.37e-42	56	522	193	694	Penicillin-binding protein 1B OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) OX=243277 GN=mrcB PE=3 SV=1
O66874	2.37e-41	43	516	63	639	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1
Q07806	7.50e-41	36	535	59	741	Penicillin-binding protein 1A OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=mrcA PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000050	0.000003	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000019_02753.