CAZyme Information: MGYG000000025_01540

Basic Information

• Species: Staphylococcus nepalensis
• Lineage: Bacteria; Firmicutes; Bacilli; Staphylococcales; Staphylococcaceae; Staphylococcus; Staphylococcus nepalensis
• CAZyme ID: MGYG000000025_01540
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 1A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
303	MGYG000000025_6|CGC1	34567.61	9.2989

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000025	2822370	Isolate	United Kingdom	Europe

• Gene Location: Start: 7295; End: 8206 Strand: -

Full Sequence      

MKNHAYESHIEENKQHIKLNQFEFIYKKLKHFFVGLFSIFGCTLLILLAISVFYFQSLTK
EATHMSDAELKSKLLVISGNEAINYNSDNLLVEYNNSMNSLIVGPKHVNNNVIKALTASE
DSLFFRHNGILPKAVLRAIGQDIFNSSDATGGSTITQQLVKNQILTNQKTYNRKANELVL
SMRAEKILTKDEILYTYLNIVPFGRDYNGLNISGIGSASFSLFGKPAEDLNIAESAYIIG
LLQSPYYYTPYNEDGSLKPNHQIQIGLNRQHYVLKRMLVENKITQYDYVRAERYDIKSRL
MTK

Enzyme Prediction     

No EC number prediction in MGYG000000025_01540.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	93	277	4.6e-51	0.9548022598870056

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam00912	Transgly	9.04e-62	99	278	12	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.
COG0744	MrcB	2.95e-61	32	299	16	262	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	1.15e-50	25	288	1	241	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
PRK11636	mrcA	2.32e-36	33	295	3	249	penicillin-binding protein 1a; Provisional
COG4953	PbpC	6.20e-32	113	292	71	235	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AWI44290.1	2.75e-213	1	303	1	303
ATH64922.1	3.90e-213	1	303	1	303
ATH59829.1	2.26e-212	1	303	1	303
AYX90113.1	3.40e-175	1	303	1	303
AVL77981.1	1.38e-174	12	303	12	303

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2JE5_A	6.62e-42	69	297	23	251	StructuralAnd Mechanistic Basis Of Penicillin Binding Protein Inhibition By Lactivicins [Streptococcus pneumoniae R6],2JE5_B Structural And Mechanistic Basis Of Penicillin Binding Protein Inhibition By Lactivicins [Streptococcus pneumoniae R6]
4OON_A	1.63e-27	85	291	24	216	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
3UDF_A	2.87e-27	85	291	24	216	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
3NB6_A	1.92e-25	105	291	29	197	Crystalstructure of Aquifex aeolicus peptidoglycan glycosyltransferase in complex with Methylphosphoryl Neryl Moenomycin [Aquifex aeolicus]
2OQO_A	7.28e-25	105	291	29	197	Crystalstructure of a peptidoglycan glycosyltransferase from a class A PBP: insight into bacterial cell wall synthesis [Aquifex aeolicus VF5],3D3H_A Crystal structure of a complex of the peptidoglycan glycosyltransferase domain from Aquifex aeolicus and neryl moenomycin A [Aquifex aeolicus],3NB7_A Crystal structure of Aquifex Aeolicus Peptidoglycan Glycosyltransferase in complex with Decarboxylated Neryl Moenomycin [Aquifex aeolicus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7GHV1	2.38e-28	101	291	77	262	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) OX=441772 GN=pbpA PE=3 SV=1
A7FY32	2.39e-28	101	291	77	262	Penicillin-binding protein 1A OS=Clostridium botulinum (strain ATCC 19397 / Type A) OX=441770 GN=pbpA PE=3 SV=1
A5I6G4	2.39e-28	101	291	77	262	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) OX=441771 GN=pbpA PE=3 SV=1
P02918	2.02e-27	85	292	51	246	Penicillin-binding protein 1A OS=Escherichia coli (strain K12) OX=83333 GN=mrcA PE=1 SV=1
Q9KNU5	2.70e-27	53	292	27	246	Penicillin-binding protein 1A OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) OX=243277 GN=mrcA PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000002	0.000046	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
33	55