CAZyme Information: MGYG000000025_01796

Basic Information

• Species: Staphylococcus nepalensis
• Lineage: Bacteria; Firmicutes; Bacilli; Staphylococcales; Staphylococcaceae; Staphylococcus; Staphylococcus nepalensis
• CAZyme ID: MGYG000000025_01796
• CAZy Family: GH73
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1547		169349.16	4.6881

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000025	2822370	Isolate	United Kingdom	Europe

• Gene Location: Start: 126208; End: 130851 Strand: -

Full Sequence      

MTKKKLTYKVPSIVALTLAGTALTTHHAHAADNNQNQTSSKNVLDDGNTLKQGEEIKSEI
SKPTTNISGTQIYNDPTQVQALSTDEPENYDAQLDELDKNTSSQNTYNAQNQEEQINSEQ
KETETNETSKQSLEKTSTTAEQIQQDLVEPTSENETATQAEKTSDTQYDQVDNITTGTED
NNASHENTETESDKASTKTGDINDAQVNGNTASVANSNASNNDAETQSEDTTPSNQSVNN
DNSGEVETDDSTTVAKDNDAANDKTEAKSDEVASQKEDTNDAKHAQDNDTTTNVKDNNTS
NDAETQSENTTPSNQLVDNDNSGEVETDDAANDETEAKSDEVASQKEDTSDAKHAQDNDT
TTNVKDNNTSNDAETQSENTTPSNQLVDNDNSDDVETDDPTTVAKDNDAANDETEAKSDE
VASQKEDTSDAKHAQNNDTTTNVKDNNTSNDAETQSEDTTPSNQLVDNDNSDDVETDDPT
TVAKDNDETEAKSDEVASQKEDTSDTKHIQAVNNKTTNVADSKFSNDKVETKSDKVSTPS
PSVSTDIEENDSTNSDNNTSQEIAHVYSNASAPKLRMARSVSSSPTTATRSAVSTKSATK
SALPKYTPKVNSSINNYIRNNNFQAPNYEQDIASYLPKYQYRYGKPEGIVMHDTANDNST
ITGEINYMKNNYNSAFVHAYVDGNRIIETANTDYLAWGAGPAANDRFIHVELVHTHDYDS
FARSINNYADYAATNLQYYGLAPDSAEYDGVGTVWTHQAVSKYLGGSDHTDPHGYLANHN
YSYDELYDLIYEKYLIKTGQAAAWGSSSSGSTSGSKNESNNSTTKPNTGKLTVSKNDGVG
RINTNNNGLYTTVYDQNGKKTNRTNQTLKVTKSATLGDKNFYLVSDYNNGNLIGWVHQAD
VDYNTAKTVTKHNQTYKIKPGEVVYTVPWGTSSQKAGTVSGTSNQTFKATKQQQIGKTNY
VYGTVNNLSGWVSVSKLSSTNNTATSPTTNTGQLTVNTLTNKQGTVAKSNHGVYTSVYDK
QGVQKSYVNGNTYKLSKKATLGNNSFYLITDNKTNTNLGWMQTRDITVKEAATQATSTPT
KSVSKIGQLNTNNAGIKTTVYDQKGKDASKFAGRTFTVTKQRTQGDNTYVLIQNSKQNTP
IGWVNTKDINTRNLSKTKATNGQYTVKGTNNGLYAIPWGTKAQQLDTQNNIKHNNFNASK
SVFVDKDEYLYGNVNNKTGWVSAKDLNAVQSQSDITPQKFDYIIYNANGSYYLDPNSTKA
AGSLKDFHESIFTVFEKQIINGVTWYHGKLSNGKVVWIKENDLRKELVKYYKSNLTLDQA
VALQHGLKFKPQIQRTPGKWEDATANEIKNAMDSNKLAKDPTQKYQFLRLDKAQNISGMD
LDKLLVGKGILEGQGEAFSEAAQAYNINEVYLVSHALLETGNGTSTLANGGDVVNNKVVT
DGSKKYYNMFGIGAVDSDAVKQGFVTAKNNGWDTVKKAIVGGAKFIANSYINQGQNTLYK
MRWNPDNPGVHQYATDVAWASHNATRIKGFYDSMDKLGKYFDVDTYK

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH73	1395	1528	1.5e-23	0.953125

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG4193	LytD	6.16e-74	1329	1547	37	245	Beta- N-acetylglucosaminidase [Carbohydrate transport and metabolism].
COG5632	CwlA	1.33e-48	631	941	4	301	N-acetylmuramoyl-L-alanine amidase CwlA [Cell wall/membrane/envelope biogenesis].
smart00047	LYZ2	2.05e-42	1383	1542	1	147	Lysozyme subfamily 2. Eubacterial enzymes distantly related to eukaryotic lysozymes.
smart00644	Ami_2	1.14e-28	644	772	1	126	Ami_2 domain.
cd06583	PGRP	2.65e-23	645	775	1	126	Peptidoglycan recognition proteins (PGRPs) are pattern recognition receptors that bind, and in certain cases, hydrolyze peptidoglycans (PGNs) of bacterial cell walls. PGRPs have been divided into three classes: short PGRPs (PGRP-S), that are small (20 kDa) extracellular proteins; intermediate PGRPs (PGRP-I) that are 40-45 kDa and are predicted to be transmembrane proteins; and long PGRPs (PGRP-L), up to 90 kDa, which may be either intracellular or transmembrane. Several structures of PGRPs are known in insects and mammals, some bound with substrates like Muramyl Tripeptide (MTP) or Tracheal Cytotoxin (TCT). The substrate binding site is conserved in PGRP-LCx, PGRP-LE, and PGRP-Ialpha proteins. This family includes Zn-dependent N-Acetylmuramoyl-L-alanine Amidase, EC:3.5.1.28. This enzyme cleaves the amide bond between N-acetylmuramoyl and L-amino acids, preferentially D-lactyl-L-Ala, in bacterial cell walls. The structure for the bacteriophage T7 lysozyme shows that two of the conserved histidines and a cysteine are zinc binding residues. Site-directed mutagenesis of T7 lysozyme indicates that two conserved residues, a Tyr and a Lys, are important for amidase activity.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ATH65592.1	0.0	1	1547	1	1481
ATH60545.1	0.0	1	1547	1	1481
AWI44963.1	0.0	1	1547	1	1481
AYX89480.1	0.0	1	1547	1	1401
CAC83649.1	0.0	1	1547	1	1401

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6FXO_A	1.27e-100	1306	1547	3	244	ChainA, Bifunctional autolysin [Staphylococcus aureus subsp. aureus Mu50]
4KNK_A	4.18e-93	618	808	33	225	ChainA, Bifunctional autolysin [Staphylococcus aureus subsp. aureus NCTC 8325],4KNK_B Chain B, Bifunctional autolysin [Staphylococcus aureus subsp. aureus NCTC 8325],4KNL_A Chain A, Bifunctional autolysin [Staphylococcus aureus subsp. aureus NCTC 8325],4KNL_B Chain B, Bifunctional autolysin [Staphylococcus aureus subsp. aureus NCTC 8325],4KNL_C Chain C, Bifunctional autolysin [Staphylococcus aureus subsp. aureus NCTC 8325],4KNL_D Chain D, Bifunctional autolysin [Staphylococcus aureus subsp. aureus NCTC 8325]
3LAT_A	3.04e-91	618	805	24	213	Crystalstructure of Staphylococcus peptidoglycan hydrolase AmiE [Staphylococcus epidermidis],3LAT_B Crystal structure of Staphylococcus peptidoglycan hydrolase AmiE [Staphylococcus epidermidis]
4EPC_A	2.24e-64	830	1228	21	334	Crystalstructure of Autolysin repeat domains from Staphylococcus epidermidis [Staphylococcus epidermidis]
6U0O_B	1.06e-56	1305	1547	40	276	ChainB, LYZ2 domain-containing protein [Staphylococcus aureus subsp. aureus NCTC 8325]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8CPQ1	0.0	618	1547	326	1335	Bifunctional autolysin OS=Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) OX=176280 GN=atl PE=3 SV=1
Q5HQB9	0.0	618	1547	326	1335	Bifunctional autolysin OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) OX=176279 GN=atl PE=3 SV=1
Q6GAG0	0.0	618	1547	229	1250	Bifunctional autolysin OS=Staphylococcus aureus (strain MSSA476) OX=282459 GN=atl PE=3 SV=1
Q6GI31	0.0	618	1547	227	1257	Bifunctional autolysin OS=Staphylococcus aureus (strain MRSA252) OX=282458 GN=atl PE=3 SV=1
Q931U5	0.0	618	1547	221	1248	Bifunctional autolysin OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) OX=158878 GN=atl PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000264	0.998933	0.000210	0.000206	0.000183	0.000158

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000025_01796.