CAZyme Information: MGYG000000033_02311

Basic Information

• Species: CAG-41 sp900066215
• Lineage: Bacteria; Firmicutes_A; Clostridia; Monoglobales_A; UBA1381; CAG-41; CAG-41 sp900066215
• CAZyme ID: MGYG000000033_02311
• CAZy Family: CBM35
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2427		269939.82	4.2124

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000033	3010191	Isolate	United Kingdom	Europe

• Gene Location: Start: 18501; End: 25784 Strand: -

Full Sequence      

MSFYKKMVSVCAAVALSTSIIPVIPVSADTGMTRYEAERQYTGGWADVVQNSIASGGYVV
ERIGGKSTDIGTVVFKIWAEEAGTYNAKLGYISKKDSDFYVRVNWYAWDTGNDTKFTTTA
SNSVQTQEMTLKLNAGWNDVKIYSTKGITDDGTDYRISVDYLDVDTEGYTLPNITEDTKA
TLNNTSHRDIYKASDGSGITGLGQSGSADFIVKADEDGAYNLGIWYSSADYRALEVYADN
VLVDTVYCPVTNESDRIDTAVVQLTLTAGEHKITFKNQYGQAPDIDKIQLKEAENNSESN
TENINLLGINTEPPFTNGIFSIDYNLNTGKADYKYGSDIKVSGFESVVKVEDSYDSGDGN
MINTVVKSSDYTDRYVTYDVLNDGFGQGALYTVVNQAENLPTMYQKFYVYKDMPYALVQV
ELGSDNEISTNFMAPISTSGENVVDIGTKTDARALFVPFSNDGYTAYNPETLNGAHTSYF
VTAIFDNNSRNSVIAGSVDHDTWKTGTSTWATADNGNINFFGAFAGINSKEFTYDFQPHG
SIKGTKVSSPKMFLGYFDDWRDGMETYGDANKRNNGKLSWGSGSIFGFSSWNAHQDKVTL
ENMKNASDEMATLNAGGFCDENGITWVNIDSYYDNLIDNSKEDPYTELKEYVEYAHNKGL
KVGLYNARHVLWDNDMQGWGVRDSAITDEYGNPVALAKTDSTYGVVKNSIPIDPTSDHFK
NDVKNFMSTYTGIGFDYLKIDFLNFCTLEGNYADSSITTGMQAYNALCKEFTKYIDPEKF
FLNYSIAPLFPSQYAHSRRLSCDIGTEKSNSLEKAKYMLNALQYGWWQDGNLYEYTDPDQ
ISFYVPTLLGFDTDTARGKYTSGVIAGGVMLLSNDFSSDKAKSGVESVAMNERINDVVRI
GKAFRPTKATSAWNTPYENDKKDPDNVYYMEDNGYTYVAVFNFDSTSKTKEFNLADVGLN
PTEEYSAVELWDNSSVTISGDSFTTNSIAARSANLYKIESGADTQENGAETLFYEDFDTT
GTTTETITSNSNGWTFGTWGDTAPFAQIGGFCGRENSLRLSAGNSWWTSGYVNVNLAKAG
AAQKSISEDEINEKLSKDITLSFKVNFDIPNPEQAGYNSIRIKDKNGNVIVQIQTPERSN
NENGITDKLKLTGVKNPDGSGDNLSYTLKDPINNVNNNTEWYEIAVNFDMTNSKYRVSVD
GQVLNTDEYGEWIESSTPLTDNIITDLYMEVYSAAWYQSMHFDDIKVVSAADDVEFNVDA
EIKNTIEDRDEIWPGDILDYTLTSSGGTIDNDKTEVSFAYRTTDQSGYTPITDKIVPEDA
YRIMMVADVVSEDGNTVRRIVYADVEPYDRTYYNEDFDNDEVYENIKNTADGWSISGNTG
NINSNNDIISQSNGMLRFYTDKYWNQFWLDKDITSAVKMKYTKDGLSDSTAEVNIAKKLG
GNFVVSFKAGFQQGDNDNGNNNEYFIRLNGKSGNILTQIHAVSPNTDNGGVHTVYLSVDG
TDYKIAEESNSDMNLRDYKFVVNEDGTGYKLYIDGTQFTDTSYGDGWILANMSELSKLSL
GIFWTSWYQTIWFDDFKIEPIMYDIDTSQFVGGTVETIRSRQSTDTAEAGNRIFVKVTPN
KGYRNILVESLKVNGTEIGENGMFIMPNETAVITGAFKENPKKLATPAGLTFINGKAEWN
TVENADGYVLQLYKKSGEEYTAEGEPINIAKNTTEYEFSELSVGDYAYKVKAIGDGDNFS
DSDEVQSEDYRQTKKLATPQIVLFENGKAKWNSVDNAQSYSVRGYKYDKAADVYTAVGET
VQGITATEYDCNITEVGTYSVHVRAESSSEYYTQGDIACSENNGMTYKYMVNLAPPASAS
ISGSGIAQWEAVENALGYDIQLYCKTEDGGEPLGKVYRVNADTTQYVFDFETDGKYYFTV
KTVGDEYYIASEKTTESNVFEYIQKPPVNAPENLEISINNDGRTLNVSWGAVSDSEGYMI
RLYKKTDNEESDDIMTDTKMINNAAQTECEFVLSAKGVYYVSIIALGNGQTSDKSKTVTS
TDYEHNPVAQKLDVPESVTFNNGKAEWSMVEGAEGYRIQLYKNGKAEGAPEIVRDRLSLD
FEFSGVGGYTYKITALGDGLYYSDSDEAESNEYVISAKLEMPVLLGFSDGKASWRAVANA
QGYKIQLYKDGTAYGKVISVESDILNCDMMEYIDSKGVYTYTVTTIGDGKYYTDSDESAQ
SVGYNYEPGTDVVQLPAPSEIKFEKGIATWTSVAHAEKYELKLYRDNSLLKKVETELFTY
DFTAYIKKDGKYYYTITSIGNGEKYTNSETVQSEVYNYKASDDNNDDKTEPEWGVKSFNL
NKQTGNFNITVTAGKERYPDTKIYVALYRNGVLVSVKSFVPEFDSNSEYSGDGTMSLPSD
MTNISAKAFIWHGEMVPITEAIQLSIK

Enzyme Prediction     

No EC number prediction in MGYG000000033_02311.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd04081	CBM35_galactosidase-like	7.43e-10	196	290	28	125	Carbohydrate Binding Module family 35 (CBM35); appended mainly to enzymes that bind alpha-D-galactose (CBM35-Gal), including glycoside hydrolase (GH) families GH27 and GH43. This family includes carbohydrate binding module family 35 (CBM35); these are non-catalytic carbohydrate binding domains that are appended mainly to enzymes that bind alpha-D-galactose (CBM35-Gal), including glycoside hydrolase (GH) families GH27 and GH43. Examples of proteins which contain CBM35s belonging to this family includes the CBM35 of an exo-beta-1,3-galactanase from Phanerochaete chrysosporium 9 (Pc1,3Gal43A) which is appended to a GH43 domain, and the CBM35 domain of two bifunctional proteins with beta-L-arabinopyranosidase/alpha-D-galactopyranosidase activities from Fusarium oxysporum 12S, Foap1 and Foap2 (Fo/AP1 and Fo/AP2), that are appended to GH27 domains. CBM35s are unique in that they display conserved specificity through extensive sequence similarity but divergent function through their appended catalytic modules. They are known to bind alpha-D-galactose (Gal), mannan (Man), xylan, glucuronic acid (GlcA), a beta-polymer of mannose, and possibly glucans, forming four subfamilies based on general ligand specificities (galacto, urono, manno, and gluco configurations). Some CBM35s bind their ligands in a calcium-dependent manner. In contrast to most CBMs that are generally rigid proteins, CBM35 undergoes significant conformational change upon ligand binding. GH43 includes beta-xylosidases and beta-xylanases, using aryl-glycosides as substrates, while family GH27 includes alpha-galactosidases, alpha-N-acetylgalactosaminidases, and isomaltodextranases.
cd14791	GH36	3.44e-06	587	801	5	218	glycosyl hydrolase family 36 (GH36). GH36 enzymes occur in prokaryotes, eukaryotes, and archaea with a wide range of hydrolytic activities, including alpha-galactosidase, alpha-N-acetylgalactosaminidase, stachyose synthase, and raffinose synthase. All GH36 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH36 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.
pfam17801	Melibiase_C	5.29e-06	933	998	13	74	Alpha galactosidase C-terminal beta sandwich domain. This domain is found at the C-terminus of alpha galactosidase enzymes.
cd14792	GH27	7.05e-06	587	855	4	227	glycosyl hydrolase family 27 (GH27). GH27 enzymes occur in eukaryotes, prokaryotes, and archaea with a wide range of hydrolytic activities, including alpha-glucosidase (glucoamylase and sucrase-isomaltase), alpha-N-acetylgalactosaminidase, and 3-alpha-isomalto-dextranase. All GH27 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH27 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.
cd04083	CBM35_Lmo2446-like	1.35e-05	193	277	17	110	Carbohydrate Binding Module 35 (CBM35) domains similar to Lmo2446. This family includes carbohydrate binding module 35 (CBM35) domains that are appended to several carbohydrate binding enzymes. Some CBM35 domains belonging to this family are appended to glycoside hydrolase (GH) family domains, including glycoside hydrolase family 31 (GH31), for example the CBM35 domain of Lmo2446, an uncharacterized protein from Listeria monocytogenes EGD-e. These CBM35s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. GH31 has a wide range of hydrolytic activities such as alpha-glucosidase, alpha-xylosidase, 6-alpha-glucosyltransferase, or alpha-1,4-glucan lyase, cleaving a terminal carbohydrate moiety from a substrate that may be a starch or a glycoprotein. Most characterized GH31 enzymes are alpha-glucosidases.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ANE45667.1	5.60e-125	194	980	59	808
ALS26483.1	1.76e-123	194	981	81	857
AWP26688.1	2.35e-117	166	998	42	866
QOT11184.1	3.11e-117	212	998	89	865
ACX66630.1	5.69e-117	212	998	89	865

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000221	0.999120	0.000173	0.000163	0.000154	0.000137

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000033_02311.