CAZyme Information: MGYG000000040_01976

Basic Information

• Species: UBA1191 sp900066305
• Lineage: Bacteria; Firmicutes_A; Clostridia; Peptostreptococcales; Anaerovoracaceae; UBA1191; UBA1191 sp900066305
• CAZyme ID: MGYG000000040_01976
• CAZy Family: GT2
• CAZyme Description: D-alanine--poly(phosphoribitol) ligase subunit 1

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2272		252353.21	4.5395

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000040	2646772	Isolate	United Kingdom	Europe

• Gene Location: Start: 66109; End: 72927 Strand: +

Full Sequence      

MNEKLIEWNDTDIEIGYKGSLDVLLHERFTENAECVAAKDQSGVFTYAQLEAASNAVSDI
VRAAEDQNVCVLVGNRAERLAYVTGIIKAGRTYVPLGMNTPVKRNMEIVSNVKPGFILTD
EENRADAELIASKCGVPADKVLIRTMSELEKADCGFTPSGRTDDSIAYIIHTSGSNGKPK
GVLSPDLGLRNLCWCCRLYFDITPDDSTCAVHNCSFDASIIDMFPFMMAGAKIHFIPDEM
KADIFTLNGYMIENGITIQELPTTLYHHFIDLENPVLHTLIAGGEKMLKYRKKSYQIFNA
YGPSEASVYSTIIKLDGEYDDIPIGRPIYNTKVLILREDGTIADIGEEGEICVTGVHLAK
GYLNEPEQQAKAFVPSVLDPDKIMYKTGDVGMWTEEGYILCSGRKDFQIKHRGFRIELDE
IRHHLMEMPEIADCAVLYHKRETGGYIACFYVPADGGEIGRERLRSYSLEFLPEYMVPGK
WLCVSEIPVTANHKIDRRALEVMLDKERSSAPAGAGAGENVTAGAAGGEAGGAAGAGNGG
NSGSTAGSIESKVRSIWAEVLDVDPDFGTDETFNGLGGHSIMSLVMLKKIKEEFGVNISF
IDFQKKNSFGDLLAMIRAEAGPAAQAAGNGREAGGAGGAGNGGNAAGSIESKVRSIWAEV
LDVDPDFGADETFNELGGHSIMSLVMLKKIKEEFGVNISFIDFQKKNSFGDLLAMIRAEV
EPAETCSFASDREHRFDDFPLTGMQQAYYFGRMDNMHLGTTPTHLYIEVDIKDFDKAKFV
RVLNRITEAHDALRLRVSDEGTQRIVPQQIITEDMITFADASQLDEEGKQRTIVAARKVI
NSIDIDYTDSPLARVTVVLTGDDSARVGLYLDGFVADGWSQDILLRDFDALWKDETQQLA
EEKYLFRDYVNFVNSLKTGEAYEKARDFWMERLPELPGVPELPLKKAADDICDPSIKNLD
RYMGMDEWNAFEKKCKVHGITTSNAMMTVFGRVLARWSRQNRFVINIPMIKRFFEQADFE
DTFGICTDFIIFDMKYDRTLGFGQEAHRNQEHMEQLIGNSLFSGMDVIREMSKQRGTLGN
ATPVVFTSLVDVPEHSYEYVKKVFFQTHTSQVWIDAIVLKSGGRIQFSWDYVADLFEDHT
VNAMIDTLVSEIRRLALHDDAWDNTAIEPVSAFPVDLDSAAGPVTEVEYRPLAEMLLDSF
AAHGENVAVISCGREYTYNELLGRSYEIAAKLQEFGLRQGGRAVILMDKCFDQVASVIGT
VLCGAAYVPLDTQNTSSRIAYCFEQTEAAAILTDHQMLATYPEIKDTCIVVDTGEMTPGE
VPGKNGFVRPEYSLDDLYGIIYTSGSTGMPKGVMLHQAGLINCMAFTRKLLHLTETDRMI
SLTNLCHDMSIYDIFGILAEGAAVVLPDKDKTKDPDNWLKITRDHKVTLWNSVPAITEMM
LTAADYSGDADITSLRVILMGGEKLKKSIPTRLRKLNPEIEIYNVGGPTEATIWSIYHKY
QEEDQKRDRIPQGRGVDNVKYFVMNDDLQLCPCDVQGLMYTEGINVSRGYLKEEEKTKQV
FITNPYTGNLMYNTGDLGKYLPNGEIDILGRNDDQIKINGKRIELGEIETCCLRVDKVSS
AVAVYSEEAGMIALFYAAGEGFDEKALREELEKQLPEYMLPAVYTRVSEMPVTSNGKIDR
KKLASQIDTEALMAEKATSDRPLNRIESALMEIYRDNLNRSDITAGDNFFRAGGDSLKAI
KLLHEITEKVDPNVQLTDIFRFPTVEKLAEHIGEGAEVIVEARHNEISDRMDLSLIQKGM
WFQAKAAQMQHSSEIFMLAGSFTIDSGSFDHRRMETAVNRLISSNPELRTRIFEENYEPY
LRYEEPFEYEVQHVKTDAGIDDVRAELEETVRKEAYSFDSFPLFSFRSAETADGKAVVAF
GIHHIISDAHSVKLLLDELEGYYTESGSAAGMPGESGVPGKGAAADAADVPDAPEMSGTD
EAVAREGRMLYNDYTVWQEESLAAGSFSDDFEFWKKELDRLTPHRFTEASGEWGDYRGAS
VDTGIDRKVMQQLERMCAESSSTIYDGIAAAVNVLMKYYFEDRHTAVGLGYAGRNLAAFE
NTIGCFAVSSMECTHTDSSMKFAELVYQTGQNIKESLAHSSLPFNVYAEKAGKGMDYSLM
PYNIMINDISGGNSASADVENVADGCGCDAGASCKAQAGSVFEDFRYSDAVVPADIMVAL
EPGKTISIRYRKNMFEQAEIDVIRSDFRTVIEAAAAESDITVAELERRLDNM

Enzyme Prediction     

No EC number prediction in MGYG000000040_01976.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK12467	PRK12467	3.71e-166	44	1956	537	2342	peptide synthase; Provisional
PRK12316	PRK12316	1.57e-163	22	2053	2002	3865	peptide synthase; Provisional
cd05930	A_NRPS	1.19e-135	1205	1683	2	444	The adenylation domain of nonribosomal peptide synthetases (NRPS). The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
cd12114	A_NRPS_TlmIV_like	1.48e-133	1205	1683	2	477	The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.
PRK05691	PRK05691	1.10e-122	24	1953	1136	2946	peptide synthase; Validated

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QND46664.1	4.52e-140	5	1772	995	2662
AFZ04852.1	8.45e-80	1125	1774	517	1185
BAZ75991.1	3.98e-71	1129	1770	516	1176
BAZ00088.1	3.98e-71	1129	1770	516	1176
BAY90071.1	1.17e-70	1129	1797	515	1202

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6MFZ_A	3.03e-148	21	1782	203	1806	Crystalstructure of dimodular LgrA in a condensation state [Brevibacillus parabrevis],6MFZ_B Crystal structure of dimodular LgrA in a condensation state [Brevibacillus parabrevis]
7LY7_A	9.84e-142	737	1772	9	1016	ChainA, BmdB, Bacillamide NRPS [Thermoactinomyces vulgaris]
7LY4_E	1.01e-141	737	1772	9	1016	ChainE, BmdB, bacillamide NRPS [Thermoactinomyces vulgaris]
6LTA_A	2.13e-137	739	1772	57	1098	ChainA, Nonribosomal peptide synthetase [Streptomyces sp. Sp080513GE-23],6LTB_A Chain A, Nonribosomal peptide synthetase [Streptomyces sp. Sp080513GE-23],6LTC_A Chain A, Nonribosomal peptide synthetase [Streptomyces sp. Sp080513GE-23],6LTD_A Chain A, Nonribosomal peptide synthetase [Streptomyces sp. Sp080513GE-23],6LTD_B Chain B, Nonribosomal peptide synthetase [Streptomyces sp. Sp080513GE-23]
6MFY_A	3.41e-136	21	1683	203	1712	Crystalstructure of a 5-domain construct of LgrA in the substrate donation state [Brevibacillus parabrevis],6MG0_A Crystal structure of a 5-domain construct of LgrA in the thiolation state [Brevibacillus parabrevis],6MG0_B Crystal structure of a 5-domain construct of LgrA in the thiolation state [Brevibacillus parabrevis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O68006	1.94e-232	2	2145	18	1994	Bacitracin synthase 1 OS=Bacillus licheniformis OX=1402 GN=bacA PE=3 SV=1
P0C064	1.19e-172	5	2266	1492	3563	Gramicidin S synthase 2 OS=Brevibacillus brevis OX=1393 GN=grsB PE=1 SV=2
P0C063	6.61e-171	5	2266	1492	3564	Gramicidin S synthase 2 OS=Aneurinibacillus migulanus OX=47500 GN=grsB PE=3 SV=2
P94459	1.79e-159	25	2161	465	2418	Plipastatin synthase subunit D OS=Bacillus subtilis (strain 168) OX=224308 GN=ppsD PE=1 SV=2
Q04747	3.14e-159	25	2166	467	2432	Surfactin synthase subunit 2 OS=Bacillus subtilis (strain 168) OX=224308 GN=srfAB PE=1 SV=3

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000056	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000040_01976.