CAZyme Information: MGYG000000042_00928

Basic Information

• Species: Phocaeicola sp900066445
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Phocaeicola; Phocaeicola sp900066445
• CAZyme ID: MGYG000000042_00928
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
860	MGYG000000042_2|CGC7	98620.47	5.3488

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000042	3613218	Isolate	United Kingdom	Europe

• Gene Location: Start: 323229; End: 325811 Strand: +

Full Sequence      

MKFIGKKLFAALILQAAVSGNYLYADVYRQNINEGWKFRQARLNNWYNATVPGVVHTDLM
DNAIIDDPFFRLNERAVQWVDKEDWIYETVIIGEDKVLNSSNQCLVFKGLDTYADVYLND
SLILKADNMFREWSVDVKGILKKGDNKLRVYFHSPIKIDMPKWEALPYQLESGNDQSENG
GLLDRKIGIFARKAGYHYGWDWGPRLVTSGIWRPVYLEAWNDVRIENVQLIQNKVNSGSA
RLSSVVEIISDDDASGAVVSVKADGKVVSQKKVDLVKGMNKVTLDYTIKNPKLWWTNGLG
NPYLYDFTTTVAMSDGTKDSVDDRIGIRSLRVVEDKDEAGTTLYFELNGKPVFMKGANYI
PSDNFLPRVTDEKYERTILDAKNANMNMLRIWGGGIYENDIFYDLCDEHGILVWQDFMFA
CSLYPGDDAFMENVRQEAIDNVRRLRNHPCIALWCGNNENLDAWFGWGWKRKYEAMGNGY
ADSLWNDFYELYFKLLPEVVEEYGSGTGYRGSSPMAYPEGKSDGVNGDSHYWGVWHGNHP
ISQYNTERSRFFSEYGFQSFPEYESVKLYAPEKRDHDINSEVMMSHQRGGSFANNRIKEY
MLKSYNEPKDFEHFLYVGMILQGDAIKTAMEAHRRDMPYCMGSLFWQHNDCWPVASWASR
DYYGRWKAQHYFAKKAYDMILVSPVVLNEKLTVHLVSDYLKAVSGKFSLKVMDMKGNVVY
DKSMSLKTPVNGSELVFESAINDVLGGKSKNDVIIYTEFETKDKTYTNVAYAAEQKDMNY
EKANISWSAKKAEGGYDVTLKSDVFARGVFMSLSGIDNFFEDNYFDLLPGKEVTINVKTD
LSLSVFKEQLKVISLVDSYN

Enzyme Prediction     

EC	3.2.1.25

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	27	790	1.9e-112	0.761968085106383

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	4.22e-117	29	835	12	782	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam17753	Ig_mannosidase	2.78e-21	782	857	1	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
PRK10340	ebgA	5.06e-19	30	459	42	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam00703	Glyco_hydro_2	3.96e-15	223	328	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
pfam17786	Mannosidase_ig	1.58e-14	689	777	1	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT74423.1	0.0	12	859	13	864
QGT72566.1	0.0	16	859	22	867
QUT31677.1	0.0	16	859	11	856
QRM99606.1	0.0	16	859	22	867
CBK67275.1	0.0	16	859	11	856

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VJX_A	8.41e-237	32	831	11	810	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	8.98e-237	32	831	13	812	Structureof a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	9.28e-237	32	831	13	812	ChainA, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	2.46e-236	32	831	11	810	Structureof the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]
2VQU_A	1.08e-234	32	831	11	810	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQU_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q4WAH4	6.36e-125	32	683	9	659	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1
B0YBU9	2.45e-124	32	683	9	659	Beta-mannosidase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=mndB PE=3 SV=1
Q0CCA0	6.39e-124	25	683	2	657	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2
A1D911	1.84e-123	32	683	9	659	Beta-mannosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndB PE=3 SV=1
I2C092	1.77e-122	32	837	9	838	Beta-mannosidase B OS=Thermothelomyces thermophilus OX=78579 GN=man9 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.030928	0.966806	0.001370	0.000289	0.000279	0.000299

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000042_00928.