CAZyme Information: MGYG000000043_00085

Basic Information

• Species: Phocaeicola sp900066455
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Phocaeicola; Phocaeicola sp900066455
• CAZyme ID: MGYG000000043_00085
• CAZy Family: GH89
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
721	MGYG000000043_1|CGC4	83418.03	6.2175

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000043	4183533	Isolate	United Kingdom	Europe

• Gene Location: Start: 113676; End: 115841 Strand: -

Full Sequence      

MKIKRLLLFLLACLCIPFSLWSNPVDGLLERICPGASHKFIIQLQKGGNDFFELDQKGNK
VVIRGNNYVNIATGLNWYLKYYAGIHLSWNGMTAQLPESLPKVDTPLRKETNLSLRYDFN
YCTYSYTMAFWDWNRWEKEIDWMALHGINLPLAAVGQECVWKNMLAKLGYTKEEINKFIA
GPAFLAWWAMNNLEGWGGPNPDSWYTQQEALQKKILKRMREYGIEPVFPGYSGMVPHDAN
QKLGLNVTEPKLWNGFTRPAFLLPTDSRFKEIAALYYKEQEKLFGKANYYSMDPFHELED
AGSVDFDAAGKAVLHAMKAVNPKATWVIQGWTENPRPEMIKNLNNGDILILDLFSECRPM
WGIPSIWKREKGYEQHDWLFCMLENFGGNIGLHGRMDQLLNNFYLTKNNPLAAHLKGIGL
TMEGSENNPVMFELMCELPWRPEKFTKEEWLKGYIKARYGTYDETVAKAWDILANGIYNC
PFGNNQQGTHESIFCGRPSLNNFQASSWSKMENYYDPTTTEDAARLMLEVADKYKGNNNF
EYDLVDIVRQSLSDRGRIVYNQTVADFKSFDKKSFAAHSQEFLNILLAQDRLLATRSEFR
VGRWIEQARNLGTTPEEKDLYEWNARVQITTWGDRVCANDGGLRDYAHKEWNGILKDFYY
KRWAAYWQTLQDVLDGKPMVELDYYAMEEPWTVAHNPYPAKGEGDCISVAKEVFNEVFKD
K

Enzyme Prediction     

No EC number prediction in MGYG000000043_00085.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH89	58	715	1.7e-260	0.995475113122172

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam05089	NAGLU	0.0	116	442	1	333	Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain. Alpha-N-acetylglucosaminidase, a lysosomal enzyme required for the stepwise degradation of heparan sulfate. Mutations on the alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B) characterized by neurological dysfunction but relatively mild somatic manifestations. The structure shows that the enzyme is composed of three domains. This central domain has a tim barrel fold.
pfam12972	NAGLU_C	3.12e-109	450	711	1	258	Alpha-N-acetylglucosaminidase (NAGLU) C-terminal domain. Alpha-N-acetylglucosaminidase, a lysosomal enzyme required for the stepwise degradation of heparan sulfate. Mutations on the alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B) characterized by neurological dysfunction but relatively mild somatic manifestations. The structure shows that the enzyme is composed of three domains. This C-terminal domain has an all alpha helical fold.
pfam12971	NAGLU_N	7.00e-35	23	101	1	81	Alpha-N-acetylglucosaminidase (NAGLU) N-terminal domain. Alpha-N-acetylglucosaminidase, a lysosomal enzyme required for the stepwise degradation of heparan sulfate. Mutations on the alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B) characterized by neurological dysfunction but relatively mild somatic manifestations. The structure shows that the enzyme is composed of three domains. This N-terminal domain has an alpha-beta fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADY36941.1	0.0	1	720	1	720
QDO68555.1	0.0	1	721	1	720
QUT88634.1	0.0	1	719	1	718
ALJ60359.1	0.0	1	719	1	718
QJR53605.1	0.0	1	712	3	747

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VC9_A	3.38e-151	23	721	172	884	Family89 Glycoside Hydrolase from Clostridium perfringens in complex with 2-acetamido-1,2-dideoxynojirmycin [Clostridium perfringens],2VCA_A Family 89 glycoside hydrolase from Clostridium perfringens in complex with beta-N-acetyl-D-glucosamine [Clostridium perfringens],2VCB_A Family 89 Glycoside Hydrolase from Clostridium perfringens in complex with PUGNAc [Clostridium perfringens],2VCC_A Family 89 Glycoside Hydrolase from Clostridium perfringens [Clostridium perfringens]
7MFK_A	4.18e-151	23	721	180	892	ChainA, Alpha-N-acetylglucosaminidase family protein [Clostridium perfringens ATCC 13124],7MFL_A Chain A, Alpha-N-acetylglucosaminidase family protein [Clostridium perfringens ATCC 13124]
4A4A_A	4.80e-150	23	721	195	907	CpGH89(E483Q, E601Q), from Clostridium perfringens, in complex with its substrate GlcNAc-alpha-1,4-galactose [Clostridium perfringens]
4XWH_A	1.07e-131	22	714	6	708	Crystalstructure of the human N-acetyl-alpha-glucosaminidase [Homo sapiens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9FNA3	1.29e-132	19	718	43	805	Alpha-N-acetylglucosaminidase OS=Arabidopsis thaliana OX=3702 GN=NAGLU PE=2 SV=1
P54802	5.57e-131	22	714	29	731	Alpha-N-acetylglucosaminidase OS=Homo sapiens OX=9606 GN=NAGLU PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000303	0.998793	0.000410	0.000161	0.000151	0.000154

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000043_00085.