CAZyme Information: MGYG000000045_00527

Basic Information

• Species: Faecalibacillus intestinalis
• Lineage: Bacteria; Firmicutes; Bacilli; Erysipelotrichales; Erysipelatoclostridiaceae; Faecalibacillus; Faecalibacillus intestinalis
• CAZyme ID: MGYG000000045_00527
• CAZy Family: GH3
• CAZyme Description: Beta-hexosaminidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2250		246726.8	4.532

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000045	2722761	Isolate	United Kingdom	Europe

• Gene Location: Start: 89499; End: 96251 Strand: -

Full Sequence      

MKRILKVVLVALMVVASTIRVDAKSTISQRVQNIIDNMSSTDKVAQMIQADTRWITPEEV
AEYKIGSILSGGGAAPSTGNQLNNWVDSANSYQQAVINSGGIPLLYGIDAVHGNNNLYGA
TIYPHNIGLAAANDQQLVENIGSATASEVRAMGANWTFTPTLGVLHNERWGRTYETFGDD
VERVTSLGNAYIKGIEKDGSTLSTAKHYLGEGLTTNGTNQGNVELSERDYNDLINANMDN
KIVKEILTPYKKAIDQGVQSIMVSYNSINGKKCHGNKDVLTTLLKEKLGFEGIIISDYNG
LDQIENQSSYKDKAITCINAGVDMLMVAEQDGDTPRWKNLYNALVEAVNEDKISEERLND
AVARILTAKENIGLLDDSSKAYANKDAQALFGGQEHRTLARQAVSESLVLLKNDTVKDGQ
TMMQALENMDNLMVAGSAGDDIGKQCGGWTITWQGSTGNTTKGTTIFSGLKAAMDKKGGT
INYSANGVYADSTSKVDAAIVVVGEDPYAESNGDRSASQLKLPASDISTIKQIENSHPDT
PIILVLTTGRPIAIADYVNDSHIKGIVNAWLPGSEGDGVADVLLGNKDFVGTNPITWTWY
PQDITSKYTDTSKVLYPVGYGLKKSQKTGDVTSPDDPNVVDLEKTNGKLEAEDFIDAHSS
IQLENNGTTVGYLQDGRYMAYKIKVPEKAAYKLTVQAARQYESTIEGAFELYLDDELVLE
KKNTSIVSTGSWTTFTAQEMNALVSLKAGVHELKLVARDKDFNFDYYTFEKAGDYVGPVV
PEEVTNVGTGAMLQEGAVEVSMSSSENSQSMSWYKGEFEISNKNATKDALDLRVADDSKQ
TTIIVNDQKTYQSVLGMGTSIEEATIHNLLKMTDENRQAFLRRLLDPVNGMGMSLIRVTI
GTSDFTAQDFYTYYDGTGKELDGKPDWNNVTGKGFSIQKDQDYGVIKVLNEMLTIAKELG
VENNLKFFASSWTPPGWMKTATSSSKSYENNDLLLKGGKLNDAYINDLAKYMVRYVEEYK
KQGIPIYAMTLQNEPLLEINYPSCAMTGTQEAKLAKAIKAELAQSTILNDQEKAVKLWAF
DHNFDGADNFMKDFFKEAGDDYNIDGIAFHPYGGNASTMGSFYDNYKDKLSMNLTERSVW
GTSGANDIITWLRNGSESYNSWVTMLDSNVGTHHWVGTPDPTLFVQDANNPQRYWATPEV
YIMSQFTKYVKPGYVRIDTNNGSSSTVTNVAFKDPETGKIVMIVTNRSGSDQKFKVMMNG
TQFNAVLPAGNVATYIWDGSIAEVKGNEIPGVLKATDAVNYDKLKVKDDGSGFGNVQDGA
WADYLIDVKEAGLYNVSIPHAIGPTSGPSVDSNIDNKQIVLKVDNQEVGRTVTKRFDTWS
KDWNAWSTTRNVQVQVKLNAGVQRLTLSLPQGDMDIGALTFTKAKDVLNVPGYINALDYS
YGENIIAENNENVGFFDDNDKLEYTVNVQKADNYKMKLEYAKAEKDAEFDIYVDDVLTTS
STLETTGSFSTYKKGTVAIDLSEGSHKIMFVPKNNGGFNLKSLSFGSYIQLENDSLKEGK
LDGQKIIVHLKDGKFNERLNKDNWKIDLPQGIDFDLDRVSDTEATITLKGQEVVDYDSNR
TLTVEINAKEVGDKDYSLSNQTIIEAVNDEESLEDIGNLDFDSDTFDLKITGGKFNQEIK
ASDLTLSEEIAQYVKVSKVEVSKDGTTATVTLERTNTNYDDLAGTITVKPSGYSDGDLDL
VSNIKLLKTDRLPDSIDVKDEAVKLSENDAYRKKGTLVNGAKGDYLDFFLNVEEEGNYVL
TYKVKDDEAVTNGLKLSGGLGLATDNLGSVSFGKYWGNTQGYAQMLNLKAGKQTLRLEVN
NPSFELTNLQIKKLTDAVEVKDVKNEVTTIKADQVIDGSKEIGWAIEGSSTKNIGFGSAG
TYQDYYVDVKTAGVYDLKINYSHNCGSDTKAIIMKVDGNTTTPLGEVTLKNTGGWSNYQD
SDTIEVKLDAGKQFIRIYDDIDGFNYRHVELTFKGKQDVTAPEISGNDAIIYVDDDTDIK
DLLALKAIDDVDGDLSDKLTISGDYDCHQAGTYTIIVTVKDEAGNAASKTFTITVVEKAK
LVIDSKEFNVGDKFDPLTGVKAYDVDGTDITDKLTVVSNDVDTSKAGTYQIVYKVIDALG
NEVEFTREVIVKKVKIDIEPSKPGDTTPSKPNDSSSNNQPSSSNNGKQTITGQVVKSTKT
GDSTPIITMMAMLLVAGLGIMVLRKRKYNR

Enzyme Prediction     

No EC number prediction in MGYG000000045_00527.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH30	838	1277	2.3e-108	0.9952038369304557
GH3	98	327	1.1e-66	0.9768518518518519
CBM6	650	771	7e-20	0.8695652173913043
CBM6	1447	1545	1.5e-16	0.7246376811594203

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG1472	BglX	3.31e-83	38	452	1	374	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
pfam00933	Glyco_hydro_3	1.88e-69	42	368	4	316	Glycosyl hydrolase family 3 N terminal domain.
PRK15098	PRK15098	3.48e-57	94	675	95	693	beta-glucosidase BglX.
PLN03080	PLN03080	3.95e-36	93	626	67	635	Probable beta-xylosidase; Provisional
COG5520	XynC	2.39e-34	843	1278	36	429	O-Glycosyl hydrolase [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCL58477.1	0.0	1	2249	2	2250
QQY26973.1	0.0	1	2248	1	2227
QQV06088.1	0.0	1	2248	1	2227
QPS14204.1	0.0	1	2248	1	2227
QMW75461.1	0.0	1	2248	1	2227

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5M6G_A	6.96e-135	22	622	42	612	Crystalstructure Glucan 1,4-beta-glucosidase from Saccharopolyspora erythraea [Saccharopolyspora erythraea D]
6JGC_A	9.48e-117	22	622	11	601	Crystalstructure of barley exohydrolaseI W286Y mutant in complex with glucose. [Hordeum vulgare subsp. vulgare],6JGD_A Crystal structure of barley exohydrolaseI W286Y mutant in complex with methyl 6-thio-beta-gentiobioside [Hordeum vulgare subsp. vulgare]
6JG7_A	3.23e-116	22	622	11	601	Crystalstructure of barley exohydrolaseI W286F in complex with methyl 2-thio-beta-sophoroside [Hordeum vulgare subsp. vulgare],6JGA_A Crystal structure of barley exohydrolaseI W286F in complex with 4'-nitrophenyl thiolaminaribioside [Hordeum vulgare subsp. vulgare],6JGB_A Crystal structure of barley exohydrolaseI W286F mutant in complex with methyl 6-thio-beta-gentiobioside [Hordeum vulgare subsp. vulgare]
1LQ2_A	3.58e-116	22	622	7	597	Crystalstructure of barley beta-D-glucan glucohydrolase isoenzyme Exo1 in complex with gluco-phenylimidazole [Hordeum vulgare],1X38_A crystal structure of barley beta-D-glucan glucohydrolase isoenzyme exo1 in complex with gluco-phenylimidazole [Hordeum vulgare],1X39_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme exo1 in complex with gluco-phenylimidazole [Hordeum vulgare]
1EX1_A	3.91e-116	22	622	7	597	Beta-d-glucanExohydrolase From Barley [Hordeum vulgare],1IEQ_A Crystal Structure Of Barley Beta-D-Glucan Glucohydrolase Isoenzyme Exo1 [Hordeum vulgare],1IEV_A Crystal Structure Of Barley Beta-d-glucan Glucohydrolase Isoenzyme Exo1 In Complex With Cyclohexitol [Hordeum vulgare],1IEW_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme Exo1 in complex with 2-deoxy-2-fluoro-alpha-D-glucoside [Hordeum vulgare],1IEX_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme Exo1 in complex with 4I,4III,4V-S-trithiocellohexaose [Hordeum vulgare],1J8V_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme Exo1 in complex with 4'-nitrophenyl 3I-thiolaminaritrioside [Hordeum vulgare],3WLH_A Crystal Structure Analysis of Plant Exohydrolase [Hordeum vulgare subsp. vulgare],3WLJ_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme EXO1 in complex with 3-deoxy-glucose [Hordeum vulgare subsp. vulgare],3WLK_X Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme EXO1 in complex with 4-deoxy-glucose [Hordeum vulgare subsp. vulgare],3WLL_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme EXO1 in complex with PEG400 [Hordeum vulgare subsp. vulgare],3WLM_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme exo1 in complex with octyl-O-glucoside [Hordeum vulgare subsp. vulgare],3WLN_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme EXO1 in complex with octyl-S-glucoside [Hordeum vulgare subsp. vulgare],3WLP_A Crystal Structure Analysis of Plant Exohydrolase [Hordeum vulgare subsp. vulgare]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q23892	7.21e-58	31	608	80	677	Lysosomal beta glucosidase OS=Dictyostelium discoideum OX=44689 GN=gluA PE=1 SV=2
A7LXU3	3.71e-55	60	622	85	657	Beta-glucosidase BoGH3B OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02659 PE=1 SV=1
T2KMH0	2.84e-45	101	733	64	703	Beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22130 PE=1 SV=1
Q56078	1.87e-44	102	675	104	693	Periplasmic beta-glucosidase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bglX PE=3 SV=2
P33363	2.49e-44	102	675	104	693	Periplasmic beta-glucosidase OS=Escherichia coli (strain K12) OX=83333 GN=bglX PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000272	0.999072	0.000179	0.000159	0.000157	0.000143

TMHMM  Annotations     

start	end
2226	2243