CAZyme Information: MGYG000000050_00229

Basic Information

• Species: Blautia_A sp900066505
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Blautia_A; Blautia_A sp900066505
• CAZyme ID: MGYG000000050_00229
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
743	MGYG000000050_2|CGC1	84214.62	5.2592

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000050	3884369	Isolate	United Kingdom	Europe

• Gene Location: Start: 23953; End: 26184 Strand: +

Full Sequence      

MNEKAYSYMDWPRIEAIVYAEEASPKDVMSPRLTPDGILIQGFFPGAEEAAVLVGNKEYP
MEQEDEAGYFAAMILGRKIPAYQFRVKTEGKTEVFYDAYAFPGMLTEEDEKAFVAGVYYN
AYEKLGAHSVIKDGIEGTSFAVWAPNAVRVSVVGDFNHWDGRRLPMHRMPMSGIFELFVP
GVQPGDIYKYEILVKGKGTHLKADPYANRAEQPRACGSVVAKVDDFVWNDQAWMADRKKY
ADRDQPVSIYETSLTKWKTAGELVDFVSDLGYTHVELHPVMEYMEDQTDGYSTSSYFAPT
SRFGTPEDFQKLVDALHQKGIGVILDWTPAQFPRYEGGLENFDGTPLYELKDPAAAVHPI
WGTLLYNYESPMVKDFLISNACFWMEVYHVDGLRLDDVDAMLYLDFGRKAGEWQPNMYGS
NENLAAIEFLKHLNSITRKRNPGVLLIAQEDGLWPELTDSVENDHLGFDYKWSGGWTRDF
LYYLAVPPVGRKNCHDQLTLSSVYAYSEHYILTLGLRDVGDLEDFKNRLPGSDQEKEAQV
REAYAYMMLHPGCKMNAPDPDMSSALQACVRDLNQVYTQHPALYRMDNDYDGFEWIQLMK
YDENVITFIRKTDKPEETLLAVCNFAGIPYKDYQTGVPFAGKYKEIFNSDDKKYGGEGKT
NPRMKRAKKEECDERDYSIKLTLPALGVVIMTCTPEEEKKPVKATQAKKAAVKKTAVKKT
AVKKTATKTTAAKKTTAIKKKLS

Enzyme Prediction     

No EC number prediction in MGYG000000050_00229.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	265	556	1.3e-108	0.9700996677740864
CBM48	124	211	1e-19	0.9210526315789473

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0296	GlgB	0.0	100	690	1	625	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK05402	PRK05402	0.0	14	691	6	721	1,4-alpha-glucan branching protein GlgB.
PRK12313	PRK12313	0.0	103	690	5	624	1,4-alpha-glucan branching protein GlgB.
PRK14705	PRK14705	4.71e-175	14	687	508	1216	glycogen branching enzyme; Provisional
PRK12568	PRK12568	3.28e-174	12	691	13	726	glycogen branching enzyme; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBL21318.1	0.0	1	712	1	710
QCU01029.1	0.0	1	697	1	697
CBL23687.1	0.0	1	709	1	707
APO30552.1	1.35e-304	97	649	10	562
AWY97839.1	1.81e-261	1	709	1	748

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GQZ_A	2.73e-167	13	695	29	775	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQW_A	3.86e-167	13	695	29	775	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A	3.86e-167	13	695	29	775	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQU_A	3.86e-167	13	695	29	775	Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR2_A	5.44e-167	13	695	29	775	Crystalstructure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GR4_A Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q1AZ86	3.56e-176	14	695	7	721	1,4-alpha-glucan branching enzyme GlgB OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) OX=266117 GN=glgB PE=3 SV=1
B3PGN4	8.02e-176	17	691	17	734	1,4-alpha-glucan branching enzyme GlgB OS=Cellvibrio japonicus (strain Ueda107) OX=498211 GN=glgB PE=3 SV=1
Q608L5	6.98e-175	12	695	12	728	1,4-alpha-glucan branching enzyme GlgB OS=Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) OX=243233 GN=glgB PE=3 SV=1
Q2W2Q6	9.86e-175	15	699	24	740	1,4-alpha-glucan branching enzyme GlgB OS=Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) OX=342108 GN=glgB PE=3 SV=1
Q3JCN0	2.30e-172	14	704	19	743	1,4-alpha-glucan branching enzyme GlgB OS=Nitrosococcus oceani (strain ATCC 19707 / BCRC 17464 / JCM 30415 / NCIMB 11848 / C-107) OX=323261 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000053	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000050_00229.